##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062705_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1603223
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.205436798249526	32.0	32.0	32.0	32.0	32.0
2	31.343723237503454	32.0	32.0	32.0	32.0	32.0
3	31.464945924553227	32.0	32.0	32.0	32.0	32.0
4	31.576518675193658	32.0	32.0	32.0	32.0	32.0
5	31.511820252079715	32.0	32.0	32.0	32.0	32.0
6	35.087029065825526	36.0	36.0	36.0	36.0	36.0
7	35.09767886314006	36.0	36.0	36.0	36.0	36.0
8	35.03827664648025	36.0	36.0	36.0	36.0	36.0
9	35.160470502232066	36.0	36.0	36.0	36.0	36.0
10	35.04966058994912	36.0	36.0	36.0	36.0	36.0
11	35.18019077820116	36.0	36.0	36.0	36.0	36.0
12	35.0919323138453	36.0	36.0	36.0	36.0	36.0
13	35.13375307115729	36.0	36.0	36.0	36.0	36.0
14	35.09448467243796	36.0	36.0	36.0	36.0	36.0
15	35.055891164236044	36.0	36.0	36.0	36.0	36.0
16	35.07337469584705	36.0	36.0	36.0	36.0	36.0
17	35.04228482251065	36.0	36.0	36.0	36.0	36.0
18	35.058863925979104	36.0	36.0	36.0	36.0	36.0
19	35.03845441339102	36.0	36.0	36.0	36.0	36.0
20	35.022622554691395	36.0	36.0	36.0	36.0	36.0
21	35.01118621676461	36.0	36.0	36.0	36.0	36.0
22	34.99256684815525	36.0	36.0	36.0	36.0	36.0
23	34.94756562249918	36.0	36.0	36.0	32.0	36.0
24	34.93393370728838	36.0	36.0	36.0	32.0	36.0
25	34.89530027949948	36.0	36.0	36.0	32.0	36.0
26	34.851838452916404	36.0	36.0	36.0	32.0	36.0
27	34.82551897022435	36.0	36.0	36.0	32.0	36.0
28	34.80567519303303	36.0	36.0	36.0	32.0	36.0
29	34.7712595191062	36.0	36.0	36.0	32.0	36.0
30	34.75940776797738	36.0	36.0	36.0	32.0	36.0
31	34.74542094268857	36.0	36.0	36.0	32.0	36.0
32	34.7084398115546	36.0	36.0	36.0	32.0	36.0
33	34.68376576433846	36.0	36.0	36.0	32.0	36.0
34	34.65944725094388	36.0	36.0	36.0	32.0	36.0
35	34.62799810132464	36.0	36.0	36.0	32.0	36.0
36	34.58876525598747	36.0	36.0	36.0	32.0	36.0
37	34.56363649972587	36.0	36.0	36.0	32.0	36.0
38	34.51698048244069	36.0	36.0	36.0	32.0	36.0
39	34.47052343934686	36.0	36.0	36.0	32.0	36.0
40	34.45747909055696	36.0	36.0	36.0	32.0	36.0
41	34.427661030312066	36.0	36.0	36.0	32.0	36.0
42	34.36769307825549	36.0	36.0	36.0	32.0	36.0
43	34.3630399513979	36.0	36.0	36.0	32.0	36.0
44	34.322960686068	36.0	36.0	36.0	32.0	36.0
45	34.25410251724183	36.0	36.0	36.0	32.0	36.0
46	34.24995649388762	36.0	36.0	36.0	32.0	36.0
47	34.196102476074756	36.0	36.0	36.0	32.0	36.0
48	34.15236869730536	36.0	36.0	36.0	32.0	36.0
49	34.12764412686195	36.0	36.0	36.0	32.0	36.0
50	33.62208501250294	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	14.0
21	68.0
22	182.0
23	548.0
24	1341.0
25	3117.0
26	6229.0
27	11297.0
28	18844.0
29	28183.0
30	40525.0
31	56838.0
32	82821.0
33	131407.0
34	280997.0
35	940806.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.0151619598505	18.08567779961933	12.36363500251722	25.53552523801295
2	15.19691959727215	20.183736316927682	39.242137593900075	25.377206491900097
3	19.213193980099998	24.30751689104899	28.424803644674206	28.054485484176805
4	11.918741185724008	16.021975732633575	36.596717986206535	35.462565095435885
5	13.678508853727772	37.50258073892403	33.73117775880211	15.087732648546085
6	33.99302900299024	35.876994587123114	16.818029283676413	13.311947126210224
7	29.75069594186211	30.5332445954181	20.65863576058976	19.057423702130023
8	27.22478407557776	34.71469658307048	19.289955296300015	18.77056404505175
9	27.057995051218704	14.541894670922261	18.755032830741573	39.64507744711746
10	15.834353673818303	27.262083939664038	31.779359452802264	25.124202933715395
11	37.28233689262193	21.246015058416702	22.436180119671437	19.035467929289936
12	24.637932464791238	23.714916764542423	29.48042786312322	22.166722907543118
13	29.90239037239361	19.806227829815313	25.534813310437787	24.756568487353288
14	22.953637765925265	20.542307589150106	25.39621749438475	31.10783715053988
15	24.670866124051365	28.313653184865732	22.885899216765228	24.12958147431767
16	24.779148003739966	26.21244829945678	25.297291767895047	23.711111928908206
17	23.1035857145263	26.541098774156808	26.204901002543004	24.15041450877389
18	24.161018149065974	25.09301575638573	27.977517787606587	22.768448306941703
19	25.032138386238223	25.410438847247075	25.875751533005705	23.681671233509
20	25.185267426926885	24.785510187915218	26.409426511470958	23.61979587368694
21	26.004679324086545	24.74147389352573	24.962341483374427	24.2915052990133
22	24.83104346681653	25.414181308526633	25.83739130489021	23.917383919766618
23	23.40560233978679	25.086154577373204	26.353414340986873	25.15482874185313
24	24.056915351139548	25.764101438165493	26.1657922821716	24.013190928523358
25	24.44507096018458	25.220758434728047	26.105351532506702	24.22881907258067
26	23.07002831171958	26.38690936943894	26.96830072921858	23.574761589622902
27	24.387686553897993	25.51366840420827	26.21188693026485	23.886758111628886
28	23.424751266667208	25.690063079184867	26.306384077573735	24.57880157657419
29	23.482759416500386	25.317750556223306	26.53323960546973	24.666250421806573
30	23.655099758424125	25.638666610945577	26.857399126634284	23.848834503996013
31	24.377020539251244	25.24159146918426	25.767906273799714	24.613481717764778
32	23.7097396931057	25.765348925258678	25.840759520041818	24.684151861593804
33	23.281290250950743	25.349998097582183	26.68050545682042	24.688206194646657
34	23.957054009329955	25.883610701692778	26.701338491276633	23.45799679770063
35	24.68920418432121	25.598559900899627	26.513342186333404	23.198893728445764
36	23.268129262117622	26.26315864979482	26.305074216125888	24.16363787196167
37	24.577554089481	25.818429501073776	25.71488807233928	23.88912833710594
38	23.841224832727576	25.816495896079335	25.84281787374557	24.499461397447515
39	24.587658734935815	25.12651078483779	25.944862317968244	24.34096816225815
40	25.24196571531222	25.488157293152607	25.900264654386817	23.369612337148357
41	23.66925873693179	25.549034663300112	27.207256881918486	23.574449717849607
42	25.166866992302385	26.391712194747708	25.95895892212125	22.482461890828663
43	23.91152073042864	25.29329980919685	26.341251341828304	24.4539281185462
44	23.41608123136956	25.96307562952877	26.145770114325956	24.475073024775718
45	23.675683295461706	26.173152456021402	25.94143172846198	24.209732520054914
46	23.554097379024714	25.318671174261798	26.136525242948565	24.990706203764923
47	24.369426068255052	25.315957490603296	26.386052586603725	23.928563854537924
48	24.477755122026068	26.071482257926686	25.236726269520833	24.214036350526406
49	23.891810434356294	25.983846289630325	25.94143172846198	24.1829115475514
50	23.62915202688584	26.896694970069667	24.997707742466268	24.476445260578224
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	32.0
2	32.0
3	147.0
4	262.0
5	296.5
6	331.0
7	297.0
8	263.0
9	392.0
10	521.0
11	807.5
12	1094.0
13	1908.0
14	2722.0
15	3836.5
16	4951.0
17	5491.0
18	6031.0
19	6305.5
20	6580.0
21	7372.0
22	8164.0
23	8961.5
24	9759.0
25	11411.5
26	13064.0
27	17081.5
28	21099.0
29	24441.5
30	27784.0
31	31610.0
32	35436.0
33	41247.5
34	47059.0
35	54383.0
36	61707.0
37	67807.5
38	73908.0
39	75768.5
40	77629.0
41	81296.5
42	84964.0
43	86307.5
44	87651.0
45	96053.0
46	104455.0
47	112732.0
48	121009.0
49	122235.0
50	123461.0
51	114272.0
52	105083.0
53	102450.0
54	99817.0
55	100803.5
56	101790.0
57	97773.0
58	93756.0
59	85565.5
60	77375.0
61	68158.0
62	58941.0
63	50679.5
64	42418.0
65	36434.0
66	30450.0
67	26731.0
68	23012.0
69	21594.5
70	20177.0
71	15315.5
72	10454.0
73	9446.5
74	8439.0
75	6017.0
76	3595.0
77	3207.5
78	2820.0
79	2407.0
80	1994.0
81	1617.5
82	1241.0
83	1076.5
84	912.0
85	740.0
86	568.0
87	418.0
88	268.0
89	174.0
90	80.0
91	54.0
92	28.0
93	20.5
94	13.0
95	13.0
96	13.0
97	9.5
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01646682963006394
2	0.003492963860922654
3	4.3662048261533174E-4
4	0.0
5	0.0
6	5.613691919339979E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.871230639779993E-4
47	6.237435465933311E-5
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1603223.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.2611103252883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.19292501817539	40.0485867507417
2	14.830454261218371	15.797729211617872
3	4.782407800476286	7.641490484450604
4	2.003009279154499	4.267299927984959
5	0.9813510739267528	2.6133923908126464
6	0.5366929570598392	1.7150917678061588
7	0.348297650705053	1.2985503720168385
8	0.2306707868043578	0.9828625779846365
9	0.1555442998666669	0.7456015904101444
>10	0.7713726305737691	7.344974113775224
>50	0.0767276375605297	2.8989378385416185
>100	0.07747701402505545	8.731196515453817
>500	0.010008244941832297	3.6510805714011183
>1k	0.003061345511619291	2.2632058870026617
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1879	0.1172014124048869	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1877	0.11707666369556824	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1732	0.10803238226996494	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1698	0.1059116542115476	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1632	0.10179494680403163	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1617	0.10085933148414163	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.237435465933311E-5	0.0
8	0.0	0.0	0.0	1.2474870931866621E-4	0.0
9	0.0	0.0	0.0	1.2474870931866621E-4	0.0
10	0.0	0.0	0.0	1.871230639779993E-4	0.0
11	0.0	0.0	0.0	1.871230639779993E-4	0.0
12	0.0	0.0	0.0	2.4949741863733243E-4	1.871230639779993E-4
13	0.0	6.237435465933311E-5	0.0	3.1187177329666555E-4	3.742461279559986E-4
14	0.0	6.237435465933311E-5	0.0	3.742461279559986E-4	3.742461279559986E-4
15	0.0	1.871230639779993E-4	0.0	4.989948372746649E-4	4.3662048261533174E-4
16	0.0	1.871230639779993E-4	0.0	5.613691919339979E-4	4.3662048261533174E-4
17	0.0	1.871230639779993E-4	0.0	5.613691919339979E-4	4.3662048261533174E-4
18	0.0	1.871230639779993E-4	0.0	6.237435465933311E-4	4.3662048261533174E-4
19	0.0	1.871230639779993E-4	0.0	6.237435465933311E-4	4.3662048261533174E-4
20	0.0	1.871230639779993E-4	0.0	8.732409652306635E-4	4.989948372746649E-4
21	0.0	1.871230639779993E-4	0.0	0.0010603640292086629	4.989948372746649E-4
22	0.0	1.871230639779993E-4	0.0	0.0016217332211426608	4.989948372746649E-4
23	0.0	1.871230639779993E-4	0.0	0.002557348541032657	4.989948372746649E-4
24	0.0	1.871230639779993E-4	0.0	0.0050523227274059815	4.989948372746649E-4
25	0.0	2.4949741863733243E-4	0.0	0.0060503124019553114	4.989948372746649E-4
26	0.0	2.4949741863733243E-4	0.0	0.007796794332416639	4.989948372746649E-4
27	0.0	2.4949741863733243E-4	0.0	0.009418527553559299	4.989948372746649E-4
28	0.0	2.4949741863733243E-4	0.0	0.013098614478459952	4.989948372746649E-4
29	0.0	2.4949741863733243E-4	0.0	0.018837055107118597	4.989948372746649E-4
30	0.0	2.4949741863733243E-4	0.0	0.03287128490546855	4.989948372746649E-4
31	0.0	2.4949741863733243E-4	0.0	0.05956750869966312	4.989948372746649E-4
32	0.0	2.4949741863733243E-4	0.0	0.09150317828524167	4.989948372746649E-4
33	0.0	2.4949741863733243E-4	0.0	0.12106862239376556	4.989948372746649E-4
34	0.0	2.4949741863733243E-4	0.0	0.1552497687470801	5.613691919339979E-4
35	0.0	2.4949741863733243E-4	0.0	0.19616734540360262	5.613691919339979E-4
36	0.0	2.4949741863733243E-4	0.0	0.25099440314915644	5.613691919339979E-4
37	0.0	3.1187177329666555E-4	0.0	0.32640499793229016	5.613691919339979E-4
38	0.0	3.1187177329666555E-4	0.0	0.4278880729630251	5.613691919339979E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAC	1185	0.0	20.796389	1
TTAGGAC	635	0.0	20.787298	3
GGCGCGA	55	0.004484369	19.9999	39
GACGGTA	400	0.0	19.799902	8
TAGGACG	1005	0.0	19.48249	4
CGCAATA	465	0.0	19.397755	36
CGACGGT	425	0.0	19.152845	7
ACGACGG	425	0.0	19.152845	6
TCCTACA	1310	0.0	19.146137	2
TAGCGGC	485	0.0	19.051453	30
TGTAGGA	1170	0.0	18.992544	2
GACCGTT	70	8.125176E-4	18.857048	7
CGTCGTA	365	0.0	18.68484	10
TAGGACC	1225	0.0	18.67746	4
CTAGCGG	495	0.0	18.666576	29
GTAGGAC	1205	0.0	18.622314	3
TACGACG	440	0.0	18.499907	5
ATACGAA	490	0.0	18.408072	40
TAGTACG	60	0.007413343	18.333242	4
GTCCTAA	625	0.0	18.306763	1
>>END_MODULE