FastQCFastQC Report
Thu 2 Feb 2017
SRR4062704_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062704_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592255
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20070.3388743024541794No Hit
GTACATGGAAGCAGTGGTATCAACG19710.3327958396298891No Hit
TCCATGTACTCTGCGTTGATACCAC17510.2956496779258934No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15030.2537758229141164No Hit
TATCAACGCAGAGTACTTTTTTTTT13480.22760466353175576No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11040.1864061932782332No Hit
GAGTACATGGAAGCAGTGGTATCAA9470.1598973415167453No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8860.14959772395336468No Hit
CATGTACTCTGCGTTGATACCACTG8670.14638964635165597No Hit
GGTATCAACGCAGAGTACTTTTTTT7540.12731002693096724No Hit
GCGCAAGACGGACCAGAGCGAAAGC7510.1268034883622764No Hit
GTCTTGCGCCGGTCCAAGAATTTCA7060.11920540983191362No Hit
CCCATGTACTCTGCGTTGATACCAC6330.10687963799376958No Hit
GAATAGGACCGCGGTTCTATTTTGT6190.10451579133987894No Hit
GTACATGGGAAGCAGTGGTATCAAC6040.10198309849642469No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG6000.10130771373817021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC504.5652323E-617.0950573
GTGTTAA402.7618E-416.62161
AGCGTAT755.820766E-815.2007438
CCGTCGT508.695779E-515.2007439
CGTCGTA508.695779E-515.20074310
ATCGCCA1200.015.04367416
GAGCGTT456.751067E-414.77850157
CGCCGGG602.5576956E-514.25310618
CGGCGTC400.005272278614.25069714
CGCGTAA400.005272278614.25069710
GCGTAAC400.005272278614.25069711
CGCCAGT1301.8189894E-1213.88764318
TGGCGAG551.9533075E-413.82119518
ACCCGCC551.9561115E-413.81885915
CTAAGCT551.96173E-413.8141884
CGTTATT551.96173E-413.8141882
CGACCAT903.621426E-813.72289510
TCTTATA707.266044E-613.5675062
CGTATAT852.687866E-713.41242110
AATAGCG852.6961789E-713.409025
Produced by FastQC (version 0.11.5)