##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062704_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	592255
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.124878641801253	32.0	32.0	32.0	32.0	32.0
2	30.81242370262809	32.0	32.0	32.0	32.0	32.0
3	30.77877434550996	32.0	32.0	32.0	32.0	32.0
4	30.85793450456307	32.0	32.0	32.0	32.0	32.0
5	30.643032139872183	32.0	32.0	32.0	32.0	32.0
6	34.26211682467856	36.0	36.0	36.0	32.0	36.0
7	34.139293041004294	36.0	36.0	36.0	32.0	36.0
8	34.14200302234679	36.0	36.0	36.0	32.0	36.0
9	34.31215101603195	36.0	36.0	36.0	32.0	36.0
10	33.98871769761336	36.0	36.0	36.0	32.0	36.0
11	34.297177735941446	36.0	36.0	36.0	32.0	36.0
12	34.08747245696533	36.0	36.0	36.0	32.0	36.0
13	34.20061628859191	36.0	36.0	36.0	32.0	36.0
14	34.10507467222733	36.0	36.0	36.0	32.0	36.0
15	34.035324311318604	36.0	36.0	36.0	32.0	36.0
16	34.02726021730505	36.0	36.0	36.0	32.0	36.0
17	33.94852386218774	36.0	36.0	36.0	32.0	36.0
18	33.97050763606892	36.0	36.0	36.0	32.0	36.0
19	33.97546664865641	36.0	36.0	36.0	32.0	36.0
20	33.97466800617977	36.0	36.0	36.0	32.0	36.0
21	33.93096385847313	36.0	36.0	36.0	32.0	36.0
22	33.888531122573895	36.0	36.0	36.0	32.0	36.0
23	33.84160032418468	36.0	36.0	36.0	32.0	36.0
24	33.823903555056525	36.0	36.0	36.0	32.0	36.0
25	33.359659268389464	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	23.0
5	58.0
6	102.0
7	23.0
8	79.0
9	90.0
10	56.0
11	29.0
12	44.0
13	42.0
14	79.0
15	99.0
16	158.0
17	237.0
18	312.0
19	437.0
20	661.0
21	1123.0
22	1663.0
23	2602.0
24	3624.0
25	5198.0
26	7198.0
27	9635.0
28	12858.0
29	17628.0
30	22540.0
31	30531.0
32	44388.0
33	62324.0
34	134198.0
35	234213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.10822831727205	17.09762230015991	11.036438026311568	25.75771135625647
2	16.643956240639337	19.987201113064152	36.766406805360674	26.60243584093584
3	19.60339665906263	23.60624015372432	28.171872704646344	28.61849048256671
4	12.637820074404688	15.015189971747835	35.54284517712064	36.80414477672684
5	14.418115206415857	36.3854717557974	33.84828210589612	15.348130931890621
6	34.58778576954652	34.76312419974391	16.761205274342142	13.88788475636742
7	29.997685337712603	30.57432346814129	20.852235000734947	18.575756193411152
8	28.23569770074929	32.489801574292194	19.9339932877967	19.340507437161815
9	26.974751571689314	14.352565402555262	18.983302913540186	39.68938011221524
10	15.73203878243702	26.559478573650118	31.78101039877757	25.92747224513529
11	37.195905286075536	21.492515111766217	21.667297732220856	19.644281869937387
12	24.050928234874448	23.949152914882358	28.983650014623862	23.016268835619332
13	29.324570344789112	19.43003385853791	25.56142121339669	25.68397458327628
14	23.368460911694207	19.545336213862377	25.411540373048798	31.674662501394625
15	25.11455581416182	26.68647624456377	23.197827031284596	25.001140909989804
16	25.69685660245326	25.808076454351685	24.106142276896865	24.388924666298188
17	23.81195483129353	25.7410913516803	25.94276151193455	24.50419230509162
18	24.739240831589303	24.458449273255322	27.05353127926673	23.748778615888643
19	24.95367959806063	25.55313998228329	25.982364436750675	23.510815982905406
20	25.085786923875304	24.003096781173195	26.477140954254864	24.43397534069664
21	26.019079046351973	24.486297606751204	25.061069515281325	24.433553831615498
22	24.954897223156873	24.627722440808416	25.712048993366892	24.70533134266782
23	23.658582770887154	24.478412887343946	26.12382156817659	25.739182773592304
24	23.90932420872541	25.492887742301956	25.895076025250457	24.702712023722178
25	24.47444985233034	24.53530970055264	25.734417765665917	25.2558226814511
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	76.0
1	76.0
2	244.0
3	412.0
4	412.0
5	412.0
6	1244.0
7	2076.0
8	2076.0
9	2076.0
10	2072.5
11	2069.0
12	2069.0
13	2069.0
14	2005.0
15	1941.0
16	1941.0
17	1941.0
18	3165.0
19	4389.0
20	4389.0
21	4389.0
22	6878.0
23	9367.0
24	9367.0
25	9367.0
26	13675.5
27	17984.0
28	17984.0
29	17984.0
30	21991.5
31	25999.0
32	25999.0
33	25999.0
34	31503.5
35	37008.0
36	37008.0
37	37008.0
38	41733.5
39	46459.0
40	46459.0
41	46459.0
42	53503.5
43	60548.0
44	60548.0
45	60548.0
46	71455.0
47	82362.0
48	82362.0
49	82362.0
50	80698.5
51	79035.0
52	79035.0
53	79035.0
54	72117.5
55	65200.0
56	65200.0
57	65200.0
58	59818.0
59	54436.0
60	54436.0
61	54436.0
62	48482.5
63	42529.0
64	42529.0
65	42529.0
66	35516.5
67	28504.0
68	28504.0
69	28504.0
70	21670.0
71	14836.0
72	14836.0
73	14836.0
74	11614.5
75	8393.0
76	8393.0
77	8393.0
78	6681.0
79	4969.0
80	4969.0
81	4969.0
82	3527.0
83	2085.0
84	2085.0
85	2085.0
86	1522.5
87	960.0
88	960.0
89	960.0
90	616.0
91	272.0
92	272.0
93	272.0
94	166.0
95	60.0
96	60.0
97	60.0
98	173.0
99	286.0
100	286.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007429232340799149
2	0.0027015390330178725
3	0.004390000928654042
4	0.014858464681598299
5	0.03748385408312298
6	0.043393470717849575
7	0.06348616727592
8	0.08391655621311765
9	0.09033271141653511
10	0.1092434846476602
11	0.11194502368067809
12	0.12764771931009447
13	0.1141400241450051
14	0.11768579412584107
15	0.10502232990856979
16	0.1070484841833332
17	0.11734810174671383
18	0.12038733315885894
19	0.12207579505449512
20	0.11363348557631425
21	0.12089387172754978
22	0.1398046449586749
23	0.13355733594482105
24	0.12511502646664022
25	0.12359541076056765
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	592255.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.592778527719794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.74509485401529	47.89672238508765
2	11.577876434211982	13.567598994621777
3	3.049035823123665	5.359544421221062
4	1.1861697016889874	2.7800391450941717
5	0.5792016874302223	1.6968518097240297
6	0.3545885291827939	1.246579629532641
7	0.23215450011042899	0.9521804046428705
8	0.17977329862052482	0.8426733657016036
9	0.12600115066664058	0.6644481763723491
>10	0.7891375945634895	8.817023550215357
>50	0.09453039209381504	3.922553126306557
>100	0.07978710857737642	8.727755988831127
>500	0.004914423354403123	1.8857318963582492
>1k	0.001734502360377573	1.6402971062906033
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2007	0.3388743024541794	No Hit
GTACATGGAAGCAGTGGTATCAACG	1971	0.3327958396298891	No Hit
TCCATGTACTCTGCGTTGATACCAC	1751	0.2956496779258934	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1503	0.2537758229141164	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1348	0.22760466353175576	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1104	0.1864061932782332	No Hit
GAGTACATGGAAGCAGTGGTATCAA	947	0.1598973415167453	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	886	0.14959772395336468	No Hit
CATGTACTCTGCGTTGATACCACTG	867	0.14638964635165597	No Hit
GGTATCAACGCAGAGTACTTTTTTT	754	0.12731002693096724	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	751	0.1268034883622764	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	706	0.11920540983191362	No Hit
CCCATGTACTCTGCGTTGATACCAC	633	0.10687963799376958	No Hit
GAATAGGACCGCGGTTCTATTTTGT	619	0.10451579133987894	No Hit
GTACATGGGAAGCAGTGGTATCAAC	604	0.10198309849642469	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	600	0.10130771373817021	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6884618956361703E-4
2	0.0	0.0	0.0	0.0	1.6884618956361703E-4
3	0.0	0.0	0.0	0.0	1.6884618956361703E-4
4	0.0	0.0	0.0	0.0	1.6884618956361703E-4
5	0.0	0.0	0.0	0.0	1.6884618956361703E-4
6	0.0	0.0	0.0	0.0	1.6884618956361703E-4
7	0.0	0.0	0.0	0.0	1.6884618956361703E-4
8	0.0	0.0	0.0	0.0	1.6884618956361703E-4
9	0.0	0.0	0.0	0.0	1.6884618956361703E-4
10	0.0	0.0	0.0	0.0	1.6884618956361703E-4
11	0.0	0.0	0.0	0.0	1.6884618956361703E-4
12	0.0	0.0	0.0	0.0	5.065385686908511E-4
13	0.0	0.0	0.0	0.0	5.065385686908511E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	50	4.5652323E-6	17.095057	3
GTGTTAA	40	2.7618E-4	16.6216	1
AGCGTAT	75	5.820766E-8	15.200743	8
CCGTCGT	50	8.695779E-5	15.200743	9
CGTCGTA	50	8.695779E-5	15.200743	10
ATCGCCA	120	0.0	15.043674	16
GAGCGTT	45	6.751067E-4	14.7785015	7
CGCCGGG	60	2.5576956E-5	14.253106	18
CGGCGTC	40	0.0052722786	14.250697	14
CGCGTAA	40	0.0052722786	14.250697	10
GCGTAAC	40	0.0052722786	14.250697	11
CGCCAGT	130	1.8189894E-12	13.887643	18
TGGCGAG	55	1.9533075E-4	13.821195	18
ACCCGCC	55	1.9561115E-4	13.818859	15
CTAAGCT	55	1.96173E-4	13.814188	4
CGTTATT	55	1.96173E-4	13.814188	2
CGACCAT	90	3.621426E-8	13.722895	10
TCTTATA	70	7.266044E-6	13.567506	2
CGTATAT	85	2.687866E-7	13.412421	10
AATAGCG	85	2.6961789E-7	13.40902	5
>>END_MODULE