FastQCFastQC Report
Thu 2 Feb 2017
SRR4062704_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062704_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592255
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10910.18421119281390616No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC8950.15111733965943724No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC7310.12342656457100404No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT6590.11126963892242363No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6400.1080615613207149No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6290.1062042532355151No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGC250.002351871235.1998634
GTATTAC450.001396029724.4484791
CGATTAG450.00139740424.4443514
TAACCGC500.002578955621.9999166
AACGGGT1351.1277734E-1021.18510441
CCGATAA1158.749339E-921.0433989
ATAACGA1158.749339E-921.04339812
CGATAAC1158.749339E-921.04339810
GACGGCC1800.020.77769911
CGGGTTA1401.8371793E-1020.42849343
GATATAT654.9225986E-420.3110431
ATCACGA1653.6379788E-1219.99992425
TTAACGG550.00448105719.99992435
TAGGACG550.00448105719.9999244
ACGGGTT1452.9467628E-1019.72406242
CGTTCCA901.0005793E-519.55548110
GCATTCG1800.019.55548122
GATAACG1252.2821041E-819.35992411
TACGCTA1252.2821041E-819.3599249
TTCAACG1605.2750693E-1119.24992638
Produced by FastQC (version 0.11.5)