##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062704_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	592255
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.217021384369907	32.0	32.0	32.0	32.0	32.0
2	31.356787194704985	32.0	32.0	32.0	32.0	32.0
3	31.45742290060869	32.0	32.0	32.0	32.0	32.0
4	31.569393251217804	32.0	32.0	32.0	32.0	32.0
5	31.495138073971514	32.0	32.0	32.0	32.0	32.0
6	35.0765312238816	36.0	36.0	36.0	36.0	36.0
7	35.09293631965961	36.0	36.0	36.0	36.0	36.0
8	35.02662873255608	36.0	36.0	36.0	36.0	36.0
9	35.157067479379656	36.0	36.0	36.0	36.0	36.0
10	35.0297557639868	36.0	36.0	36.0	36.0	36.0
11	35.16598593511241	36.0	36.0	36.0	36.0	36.0
12	35.06886729533731	36.0	36.0	36.0	36.0	36.0
13	35.109952638643826	36.0	36.0	36.0	36.0	36.0
14	35.08136697875071	36.0	36.0	36.0	36.0	36.0
15	35.03544756903698	36.0	36.0	36.0	36.0	36.0
16	35.053880507551646	36.0	36.0	36.0	36.0	36.0
17	35.03432643033828	36.0	36.0	36.0	36.0	36.0
18	35.0398916007463	36.0	36.0	36.0	36.0	36.0
19	35.01876050012241	36.0	36.0	36.0	36.0	36.0
20	35.00434610091937	36.0	36.0	36.0	36.0	36.0
21	34.984206127428216	36.0	36.0	36.0	36.0	36.0
22	34.967637250846344	36.0	36.0	36.0	32.0	36.0
23	34.926892976842744	36.0	36.0	36.0	32.0	36.0
24	34.91225063528379	36.0	36.0	36.0	32.0	36.0
25	34.88780001857308	36.0	36.0	36.0	32.0	36.0
26	34.83210103755984	36.0	36.0	36.0	32.0	36.0
27	34.82293606639032	36.0	36.0	36.0	32.0	36.0
28	34.80864830182945	36.0	36.0	36.0	32.0	36.0
29	34.78781437049919	36.0	36.0	36.0	32.0	36.0
30	34.77967091877654	36.0	36.0	36.0	32.0	36.0
31	34.7570522832226	36.0	36.0	36.0	32.0	36.0
32	34.72347384150408	36.0	36.0	36.0	32.0	36.0
33	34.68892284573368	36.0	36.0	36.0	32.0	36.0
34	34.66860896066728	36.0	36.0	36.0	32.0	36.0
35	34.63685068087226	36.0	36.0	36.0	32.0	36.0
36	34.6147014377253	36.0	36.0	36.0	32.0	36.0
37	34.59897172670556	36.0	36.0	36.0	32.0	36.0
38	34.55330558627618	36.0	36.0	36.0	32.0	36.0
39	34.533837620619494	36.0	36.0	36.0	32.0	36.0
40	34.49919713636862	36.0	36.0	36.0	32.0	36.0
41	34.480056732319696	36.0	36.0	36.0	32.0	36.0
42	34.432139872183434	36.0	36.0	36.0	32.0	36.0
43	34.42347468573503	36.0	36.0	36.0	32.0	36.0
44	34.388525212957255	36.0	36.0	36.0	32.0	36.0
45	34.30971794244034	36.0	36.0	36.0	32.0	36.0
46	34.33214240487628	36.0	36.0	36.0	32.0	36.0
47	34.28973837282927	36.0	36.0	36.0	32.0	36.0
48	34.26327510953897	36.0	36.0	36.0	32.0	36.0
49	34.26748106812099	36.0	36.0	36.0	32.0	36.0
50	33.690437396054065	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	16.0
22	47.0
23	116.0
24	381.0
25	883.0
26	1882.0
27	3721.0
28	6350.0
29	9991.0
30	14478.0
31	21385.0
32	31746.0
33	50287.0
34	107661.0
35	343309.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.56565793028911	17.788435557957307	11.591292893812483	26.054613617941097
2	16.173222655986166	20.553600615974823	36.52690161963555	26.74627510840346
3	18.910056715818126	24.0333912479675	28.597503423379617	28.45904861283476
4	12.388244928282582	15.451959037914412	35.73764679065605	36.422149243146954
5	14.165688765818777	37.02560552464732	33.361812057306395	15.446893652227503
6	34.66210329386815	35.08287012960699	16.635992786854246	13.619033789670612
7	30.451579133987895	30.706368034039393	20.36504546183654	18.477007370136175
8	28.641041443297226	32.67241306531815	19.399414103722215	19.28713138766241
9	27.393099256232535	14.32220918354425	18.439523516053054	39.845168044170165
10	15.84959181433673	26.829152983090054	31.689559395868333	25.63169580670488
11	37.817325307511126	21.359549518366244	21.404800297169295	19.41832487695334
12	24.1985293496889	24.209335505820974	28.556449502325854	23.035685642164268
13	29.70916243847667	19.351461785886148	25.3132518931879	25.62612388244928
14	23.467763041257566	19.618069919207098	25.05018952984778	31.86397750968755
15	25.466733079501232	26.92607069589957	22.652067099475733	24.955129125123467
16	25.9555427982879	25.868924703041763	23.771686182472077	24.403846316198262
17	23.994731998885616	26.173523229014528	25.404766527931383	24.426978244168474
18	25.211775333260167	24.857029488987006	26.250179399076412	23.681015778676414
19	25.286743041426412	25.97428472532946	25.18746148196301	23.55151075128112
20	25.60214772353125	24.145511646165925	25.710209284851963	24.542131345450862
21	26.519657917619945	24.74559100387502	24.188398578315084	24.546352500189954
22	25.40780575934353	24.885564495023257	24.876784493165953	24.829845252467265
23	24.024617774438376	24.777165241323416	25.318317278874808	25.879899705363403
24	24.198698195878464	25.893913939097178	25.163823015424096	24.743564849600254
25	24.798608707397996	24.956479894639976	24.96137643413732	25.283534963824707
26	24.19751627255152	25.601978877341686	25.62966965243012	24.570835197676676
27	25.25398688065107	25.425196916868583	24.900085267325732	24.420730935154623
28	24.12761395007218	25.116039543777596	25.880406243932093	24.875940262218133
29	24.027994698229648	25.684038125469606	25.727600442377017	24.560366733923733
30	24.51123249276072	25.524816168711112	25.805438535765845	24.158512802762324
31	24.354712075035245	25.547779250491764	24.77024254755131	25.327266126921682
32	24.333099762771106	25.61464234155896	24.909202961562166	25.143054934107774
33	24.01499354163325	25.06724299499371	25.42333960878338	25.49442385458966
34	24.452980557361272	25.423170762593816	25.74701775417683	24.37683092586808
35	25.421819993077303	25.231699183628674	25.389570370870658	23.956910452423365
36	24.26015820887962	25.84545508269242	25.14423685743472	24.75014985099324
37	25.309706123207064	25.66766004508193	24.995652210618736	24.026981621092265
38	24.403677470008695	25.262598036318817	25.30295227562452	25.03077221804797
39	24.719588690682222	25.28421034858296	24.947699892782673	25.04850106795215
40	24.947024508024416	25.528024246312818	25.318317278874808	24.206633966787955
41	23.79600003376924	25.87399008872867	25.76643506597665	24.56357481152544
42	25.57411925606369	25.753940447948942	25.235582645988636	23.436357649998733
43	24.760280622367052	24.522545187461482	25.37656921427426	25.340604975897207
44	24.090298942178624	25.382478830908983	25.580535411267107	24.94668681564529
45	24.43052401414931	25.624266574364086	25.26090957442318	24.684299837063428
46	23.935845093490293	25.27040762915911	25.739969675173153	25.053777602177448
47	24.31520206667736	25.054241838397314	26.210162852149832	24.420393242775493
48	24.789153320782432	26.246295936716447	24.77361947134258	24.19093127115854
49	23.806130805143056	25.718820440519707	25.832453926096022	24.64259482824121
50	23.78080387670851	26.448573671813662	24.899241036377912	24.871381415099915
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	12.5
2	16.0
3	43.0
4	70.0
5	86.0
6	102.0
7	100.0
8	98.0
9	122.0
10	146.0
11	211.5
12	277.0
13	465.5
14	654.0
15	922.5
16	1191.0
17	1414.5
18	1638.0
19	1908.0
20	2178.0
21	2743.0
22	3308.0
23	3799.5
24	4291.0
25	4466.5
26	4642.0
27	5483.0
28	6324.0
29	7406.0
30	8488.0
31	9478.0
32	10468.0
33	11899.0
34	13330.0
35	15162.0
36	16994.0
37	19557.0
38	22120.0
39	23976.0
40	25832.0
41	28138.5
42	30445.0
43	31695.0
44	32945.0
45	36590.5
46	40236.0
47	43188.0
48	46140.0
49	46524.0
50	46908.0
51	44185.5
52	41463.0
53	39911.0
54	38359.0
55	39079.5
56	39800.0
57	38434.0
58	37068.0
59	34154.0
60	31240.0
61	27433.5
62	23627.0
63	20660.0
64	17693.0
65	15182.5
66	12672.0
67	11300.0
68	9928.0
69	9173.5
70	8419.0
71	6572.0
72	4725.0
73	4117.0
74	3509.0
75	2547.5
76	1586.0
77	1440.0
78	1294.0
79	1083.0
80	872.0
81	692.0
82	512.0
83	405.0
84	298.0
85	248.5
86	199.0
87	144.0
88	89.0
89	64.5
90	40.0
91	24.0
92	8.0
93	5.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01604038800854362
2	0.0038834623599631915
3	6.753847582544681E-4
4	0.0
5	0.0
6	5.065385686908511E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.6884618956361703E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	592255.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.41829573840928
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.41551607340791	51.442360557036
2	11.339757844031876	14.156167174214563
3	2.871907735022355	5.377787590141516
4	1.1265254345602067	2.812631909248761
5	0.5712121255172198	1.782704368994959
6	0.3334436598374083	1.2487790983097338
7	0.22326944024713336	0.9755268565486268
8	0.15474698458046734	0.7727234438536538
9	0.11822874868135962	0.6641673299977654
>10	0.6927708499220986	8.22966371143714
>50	0.08472916543625458	3.7717225940794603
>100	0.06517626120550878	7.601584666224463
>500	0.0024441097952054547	0.9792475106871982
>1k	2.7156775502282834E-4	0.1849331892261958
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1091	0.18421119281390616	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	895	0.15111733965943724	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	731	0.12342656457100404	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	659	0.11126963892242363	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	640	0.1080615613207149	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	629	0.1062042532355151	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.6884618956361703E-4	0.0
5	0.0	0.0	0.0	1.6884618956361703E-4	0.0
6	0.0	0.0	0.0	1.6884618956361703E-4	0.0
7	0.0	0.0	0.0	1.6884618956361703E-4	0.0
8	0.0	0.0	0.0	1.6884618956361703E-4	0.0
9	0.0	0.0	0.0	1.6884618956361703E-4	0.0
10	0.0	0.0	0.0	1.6884618956361703E-4	0.0
11	0.0	0.0	0.0	1.6884618956361703E-4	0.0
12	0.0	0.0	0.0	1.6884618956361703E-4	1.6884618956361703E-4
13	0.0	0.0	0.0	1.6884618956361703E-4	1.6884618956361703E-4
14	0.0	0.0	0.0	1.6884618956361703E-4	1.6884618956361703E-4
15	0.0	0.0	0.0	5.065385686908511E-4	1.6884618956361703E-4
16	0.0	0.0	0.0	5.065385686908511E-4	1.6884618956361703E-4
17	0.0	0.0	0.0	5.065385686908511E-4	1.6884618956361703E-4
18	0.0	0.0	0.0	0.0011819233269453192	1.6884618956361703E-4
19	0.0	0.0	0.0	0.0011819233269453192	1.6884618956361703E-4
20	0.0	0.0	0.0	0.0013507695165089362	1.6884618956361703E-4
21	0.0	0.0	0.0	0.0018573080851997873	1.6884618956361703E-4
22	0.0	0.0	0.0	0.0025326928434542552	1.6884618956361703E-4
23	0.0	0.0	0.0	0.0037146161703995747	1.6884618956361703E-4
24	0.0	0.0	0.0	0.008273463288617234	1.6884618956361703E-4
25	0.0	0.0	0.0	0.00962423280512617	1.6884618956361703E-4
26	0.0	0.0	0.0	0.013338848975525746	1.6884618956361703E-4
27	0.0	0.0	0.0	0.015196157060725532	1.6884618956361703E-4
28	0.0	0.0	0.0	0.021950004643270214	1.6884618956361703E-4
29	0.0	0.0	0.0	0.03360039172315979	1.6884618956361703E-4
30	0.0	0.0	0.0	0.059433858726393195	1.6884618956361703E-4
31	0.0	0.0	0.0	0.11160733130155086	1.6884618956361703E-4
32	0.0	0.0	0.0	0.17087234383838043	1.6884618956361703E-4
33	0.0	0.0	0.0	0.2311504335125917	1.6884618956361703E-4
34	0.0	0.0	0.0	0.29615621649458423	1.6884618956361703E-4
35	0.0	0.0	0.0	0.37838431081206575	1.6884618956361703E-4
36	0.0	0.0	0.0	0.48847202640754406	1.6884618956361703E-4
37	0.0	0.0	0.0	0.6429662898582537	1.6884618956361703E-4
38	0.0	0.0	0.0	0.8418671011641945	1.6884618956361703E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGC	25	0.0023518712	35.199863	4
GTATTAC	45	0.0013960297	24.448479	1
CGATTAG	45	0.001397404	24.44435	14
TAACCGC	50	0.0025789556	21.999916	6
AACGGGT	135	1.1277734E-10	21.185104	41
CCGATAA	115	8.749339E-9	21.043398	9
ATAACGA	115	8.749339E-9	21.043398	12
CGATAAC	115	8.749339E-9	21.043398	10
GACGGCC	180	0.0	20.777699	11
CGGGTTA	140	1.8371793E-10	20.428493	43
GATATAT	65	4.9225986E-4	20.311043	1
ATCACGA	165	3.6379788E-12	19.999924	25
TTAACGG	55	0.004481057	19.999924	35
TAGGACG	55	0.004481057	19.999924	4
ACGGGTT	145	2.9467628E-10	19.724062	42
CGTTCCA	90	1.0005793E-5	19.555481	10
GCATTCG	180	0.0	19.555481	22
GATAACG	125	2.2821041E-8	19.359924	11
TACGCTA	125	2.2821041E-8	19.359924	9
TTCAACG	160	5.2750693E-11	19.249926	38
>>END_MODULE