FastQCFastQC Report
Thu 2 Feb 2017
SRR4062703_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062703_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010714
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT37090.36696830161648103No Hit
GTCCTACAGTGGACATTTCTAAATT35460.35084108857698615No Hit
CTGTAGGACGTGGAATATGGCAAGA31470.3113640456152779No Hit
CTTTAGGACGTGAAATATGGCGAGG29580.2926643936860477No Hit
GTCCTACAGTGTGCATTTCTCATTT25920.2564523693151574No Hit
CTGTAGGACCTGGAATATGGCGAGA18730.18531454001824452No Hit
GTATCAACGCAGAGTACTTTTTTTT15690.15523679299980014No Hit
CTGAAGGACCTGGAATATGGCGAGA14780.14623325688572633No Hit
GTCCTTCAGTGTGCATTTCTCATTT13780.1363392611559749No Hit
ATTTAGAAATGTCCACTGTAGGACG13490.13347000239434698No Hit
GAATATGGCAAGAAAACTGAAAATC11660.11536399020890183No Hit
TTTCTAAATTTTCCACCTTTTTCAG11490.11368201093484409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAA555.8831574E-717.27872318
GGCGAAA350.002164467516.29136519
TTACCGC350.002164467516.29136516
ATTACCG350.002168054216.28732915
CCAACGT400.005265268514.25494619
CGCCACC400.005275668614.25070714
AAGACGG400.00528087514.2485885
CTTCGAA500.001498352313.30065913
CAAGACG500.001500592313.2980224
TCCAACG1153.7471182E-1013.22197818
GACAGTC604.0915245E-412.6660397
CCACCTT4250.012.29472613
ACCTCCG550.00305920212.09510519
TCGAACC550.003064886712.09210815
TTCGAAC550.003066024712.09150814
TCTTCGA550.00306716312.09090912
CGTCTTC550.003068301812.0903110
TCGTCTT550.003068301812.090319
AGGACGA550.003069440812.089715
CCAACGA802.8538718E-511.87912219