##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062703_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1010714
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.180180545634077	32.0	32.0	32.0	32.0	32.0
2	30.946826698749597	32.0	32.0	32.0	32.0	32.0
3	30.96397794034712	32.0	32.0	32.0	32.0	32.0
4	30.885725338720945	32.0	32.0	32.0	32.0	32.0
5	30.923912204639493	32.0	32.0	32.0	32.0	32.0
6	34.39220788472308	36.0	36.0	36.0	32.0	36.0
7	34.354263421699905	36.0	36.0	36.0	32.0	36.0
8	34.26913053544326	36.0	36.0	36.0	32.0	36.0
9	34.4371760953148	36.0	36.0	36.0	32.0	36.0
10	34.20462366208443	36.0	36.0	36.0	32.0	36.0
11	34.48898996155193	36.0	36.0	36.0	32.0	36.0
12	34.28865237841763	36.0	36.0	36.0	32.0	36.0
13	34.35031274920502	36.0	36.0	36.0	32.0	36.0
14	34.28719400344707	36.0	36.0	36.0	32.0	36.0
15	34.217081192107756	36.0	36.0	36.0	32.0	36.0
16	34.31385733253917	36.0	36.0	36.0	32.0	36.0
17	34.21898677568531	36.0	36.0	36.0	32.0	36.0
18	34.259467069814015	36.0	36.0	36.0	32.0	36.0
19	34.19708839493665	36.0	36.0	36.0	32.0	36.0
20	34.14065205389458	36.0	36.0	36.0	32.0	36.0
21	34.09711847268367	36.0	36.0	36.0	32.0	36.0
22	34.06535182059415	36.0	36.0	36.0	32.0	36.0
23	34.046701638643576	36.0	36.0	36.0	32.0	36.0
24	34.039130753111166	36.0	36.0	36.0	32.0	36.0
25	33.36275345943561	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	8.0
4	43.0
5	87.0
6	182.0
7	31.0
8	139.0
9	178.0
10	141.0
11	42.0
12	79.0
13	52.0
14	185.0
15	361.0
16	597.0
17	753.0
18	1055.0
19	1231.0
20	1551.0
21	1889.0
22	2463.0
23	3358.0
24	4588.0
25	6288.0
26	8756.0
27	11651.0
28	16547.0
29	23253.0
30	32302.0
31	47623.0
32	73767.0
33	114870.0
34	263116.0
35	393528.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.41866540014249	20.752790311090003	11.944807250850946	24.883737037916568
2	12.910723102206717	22.529428002932658	44.03232652641223	20.52752236844839
3	18.09972077707483	28.005505302465117	30.294796875711167	23.599977044748886
4	10.687907442517702	18.08391683125992	40.613632675356136	30.614543050866246
5	10.884082399413272	40.32228531385751	36.65235805124378	12.141274235485431
6	28.03159208633877	39.78696425548848	20.163958929099937	12.017484729072816
7	25.743384928547524	33.01486472839743	24.10325511216365	17.138495230891387
8	23.217100937200442	39.79437838180421	21.92795519183693	15.06056548915842
9	26.91357706797447	15.303466662441123	21.651190612954725	36.131765656629675
10	13.716932500995515	30.816343511707473	36.08961037160014	19.37711361569687
11	32.84458814253054	23.01411358224272	26.824127014315657	17.31717126091108
12	22.696059927854346	26.099151233421214	33.97305386551899	17.231734973205448
13	28.45384537661293	23.83719432827009	27.044431985006412	20.66452831011057
14	19.18042303303113	24.37006672795283	29.227853769099095	27.22165646991695
15	20.60491942760866	35.33338814111485	24.22896842180333	19.832724009473164
16	19.278240947553908	29.77340284016565	31.423678348317978	19.524677863962463
17	19.03382101933606	31.109671398895056	30.06862077881166	19.78788680295722
18	19.01896953461831	28.91853442810043	33.807241472229265	18.255254565051988
19	22.200558951999675	27.798103605250308	29.104834706936572	20.896502735813446
20	22.555148643039765	29.02251512879161	30.219940782506967	18.202395445661658
21	21.629437219144535	28.134881951544262	27.837581334157573	22.398099495153634
22	21.7461355529132	31.249504558065794	28.54954419342053	18.454815695600477
23	19.623395975175757	30.75249470939311	29.787503863926386	19.836605451504752
24	21.39505668916268	28.684894646529894	30.951965792320706	18.968082871986716
25	20.328553893280418	29.81960445535089	30.393612795016413	19.45822885635228
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	129.0
1	129.0
2	327.0
3	525.0
4	525.0
5	525.0
6	1144.0
7	1763.0
8	1763.0
9	1763.0
10	2540.5
11	3318.0
12	3318.0
13	3318.0
14	5019.0
15	6720.0
16	6720.0
17	6720.0
18	11318.5
19	15917.0
20	15917.0
21	15917.0
22	25693.0
23	35469.0
24	35469.0
25	35469.0
26	51788.5
27	68108.0
28	68108.0
29	68108.0
30	88596.5
31	109085.0
32	109085.0
33	109085.0
34	120150.5
35	131216.0
36	131216.0
37	131216.0
38	134926.0
39	138636.0
40	138636.0
41	138636.0
42	138000.5
43	137365.0
44	137365.0
45	137365.0
46	133988.5
47	130612.0
48	130612.0
49	130612.0
50	115240.5
51	99869.0
52	99869.0
53	99869.0
54	78690.0
55	57511.0
56	57511.0
57	57511.0
58	46192.5
59	34874.0
60	34874.0
61	34874.0
62	27251.0
63	19628.0
64	19628.0
65	19628.0
66	14924.0
67	10220.0
68	10220.0
69	10220.0
70	7350.5
71	4481.0
72	4481.0
73	4481.0
74	3389.0
75	2297.0
76	2297.0
77	2297.0
78	1865.5
79	1434.0
80	1434.0
81	1434.0
82	1036.5
83	639.0
84	639.0
85	639.0
86	497.5
87	356.0
88	356.0
89	356.0
90	233.5
91	111.0
92	111.0
93	111.0
94	74.0
95	37.0
96	37.0
97	37.0
98	215.5
99	394.0
100	394.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007321556840016068
2	0.00267137884703289
3	0.005144877779470751
4	0.014049473936247048
5	0.0363109643281878
6	0.04561132031415415
7	0.0664876513039297
8	0.08884808165316796
9	0.09715903806615916
10	0.11754066926944713
11	0.11773854918404217
12	0.13525092162570224
13	0.12031098807377755
14	0.12288342696351291
15	0.11130745195970373
16	0.11130745195970373
17	0.12397176649378558
18	0.12921558423055385
19	0.13109544341920662
20	0.12021204811648004
21	0.12842406457217373
22	0.14979509534843685
23	0.1363392611559749
24	0.13356894235164446
25	0.13069968359001657
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1010714.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.96250601999365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.65975016023347	62.02858304316469
2	8.202215495636453	11.476951019815012
3	1.3621131169625755	2.8589054143621953
4	0.48632016694614355	1.3609671043045555
5	0.2559371387719681	0.8953001806036885
6	0.16909546067733933	0.7098205311355172
7	0.12588078218469687	0.6164854486978857
8	0.09014016911808594	0.5045145699653867
9	0.07006625294676017	0.4411809579227496
>10	0.4843291129981506	6.723249897804975
>50	0.05105493684945433	2.465461122400438
>100	0.03643415283945195	4.98111107144965
>500	0.0048200814980674115	2.2671437093255955
>1k	0.0018429723374963633	2.6703259290477295
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	3709	0.36696830161648103	No Hit
GTCCTACAGTGGACATTTCTAAATT	3546	0.35084108857698615	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3147	0.3113640456152779	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2958	0.2926643936860477	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2592	0.2564523693151574	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1873	0.18531454001824452	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1569	0.15523679299980014	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1478	0.14623325688572633	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1378	0.1363392611559749	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1349	0.13347000239434698	No Hit
GAATATGGCAAGAAAACTGAAAATC	1166	0.11536399020890183	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1149	0.11368201093484409	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGCGAA	55	5.8831574E-7	17.278723	18
GGCGAAA	35	0.0021644675	16.291365	19
TTACCGC	35	0.0021644675	16.291365	16
ATTACCG	35	0.0021680542	16.287329	15
CCAACGT	40	0.0052652685	14.254946	19
CGCCACC	40	0.0052756686	14.250707	14
AAGACGG	40	0.005280875	14.248588	5
CTTCGAA	50	0.0014983523	13.300659	13
CAAGACG	50	0.0015005923	13.298022	4
TCCAACG	115	3.7471182E-10	13.221978	18
GACAGTC	60	4.0915245E-4	12.666039	7
CCACCTT	425	0.0	12.294726	13
ACCTCCG	55	0.003059202	12.095105	19
TCGAACC	55	0.0030648867	12.092108	15
TTCGAAC	55	0.0030660247	12.091508	14
TCTTCGA	55	0.003067163	12.090909	12
CGTCTTC	55	0.0030683018	12.09031	10
TCGTCTT	55	0.0030683018	12.09031	9
AGGACGA	55	0.0030694408	12.08971	5
CCAACGA	80	2.8538718E-5	11.879122	19
>>END_MODULE