FastQCFastQC Report
Thu 2 Feb 2017
SRR4062703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010714
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA17350.17166082591118753No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA14160.14009897953328043No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC13770.13624032119867738No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA13170.1303039237608265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAA207.856421E-443.99955438
AATGGCG300.005744857229.33303816
CGTAAAC300.005744857229.33303840
CCGACAC551.5934755E-423.99975836
TAGGACC9850.022.1125454
ACCGGGG500.002580299521.99977738
CACCGCA500.002580299521.9997779
GTATCAA21850.021.9548661
GTAGGAC26500.020.8385543
GACGTGG12350.020.4856247
TGTAGGA27800.020.2607942
CGGAAGC550.004483374319.99979838
ATTGCGA550.004483374319.99979817
TGGTCGG550.004483374319.9997986
CTGTAGG27400.019.7565121
GTCCTAC23600.019.4876751
AGGACCT16650.019.2910965
GGACGTG25750.019.0522356
TAGGACG26100.019.0505584
GTCCTAA14450.019.035661
Produced by FastQC (version 0.11.5)