##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062703_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1010714
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.259124737561763	32.0	32.0	32.0	32.0	32.0
2	31.290993297807294	32.0	32.0	32.0	32.0	32.0
3	31.434860900314035	32.0	32.0	32.0	32.0	32.0
4	31.556016835623133	32.0	32.0	32.0	32.0	32.0
5	31.48586642710005	32.0	32.0	32.0	32.0	32.0
6	35.073165109021936	36.0	36.0	36.0	36.0	36.0
7	35.098910275310324	36.0	36.0	36.0	36.0	36.0
8	35.026915625983214	36.0	36.0	36.0	36.0	36.0
9	35.19547864183142	36.0	36.0	36.0	36.0	36.0
10	35.05732779005733	36.0	36.0	36.0	36.0	36.0
11	35.19384613253601	36.0	36.0	36.0	36.0	36.0
12	35.11421133970639	36.0	36.0	36.0	36.0	36.0
13	35.159495168761886	36.0	36.0	36.0	36.0	36.0
14	35.12569530054991	36.0	36.0	36.0	36.0	36.0
15	35.08374080105747	36.0	36.0	36.0	36.0	36.0
16	35.11314476696672	36.0	36.0	36.0	36.0	36.0
17	35.10350900452551	36.0	36.0	36.0	36.0	36.0
18	35.10829275146085	36.0	36.0	36.0	36.0	36.0
19	35.08730956531719	36.0	36.0	36.0	36.0	36.0
20	35.079564545459945	36.0	36.0	36.0	36.0	36.0
21	35.05821528147428	36.0	36.0	36.0	36.0	36.0
22	35.0505226997944	36.0	36.0	36.0	36.0	36.0
23	35.008390108378826	36.0	36.0	36.0	36.0	36.0
24	35.00337879954171	36.0	36.0	36.0	36.0	36.0
25	34.97649879194312	36.0	36.0	36.0	36.0	36.0
26	34.93044817821857	36.0	36.0	36.0	36.0	36.0
27	34.90989241269043	36.0	36.0	36.0	36.0	36.0
28	34.8802974926636	36.0	36.0	36.0	32.0	36.0
29	34.84844377341167	36.0	36.0	36.0	32.0	36.0
30	34.82643062231254	36.0	36.0	36.0	32.0	36.0
31	34.81581436489452	36.0	36.0	36.0	32.0	36.0
32	34.79467386421876	36.0	36.0	36.0	32.0	36.0
33	34.797092946174686	36.0	36.0	36.0	32.0	36.0
34	34.77501944170161	36.0	36.0	36.0	32.0	36.0
35	34.76039116901517	36.0	36.0	36.0	32.0	36.0
36	34.705874263144665	36.0	36.0	36.0	32.0	36.0
37	34.674712134194245	36.0	36.0	36.0	32.0	36.0
38	34.63888597565681	36.0	36.0	36.0	32.0	36.0
39	34.57097259956823	36.0	36.0	36.0	32.0	36.0
40	34.59563239452506	36.0	36.0	36.0	32.0	36.0
41	34.562069982210595	36.0	36.0	36.0	32.0	36.0
42	34.55677471569603	36.0	36.0	36.0	32.0	36.0
43	34.536319868924345	36.0	36.0	36.0	32.0	36.0
44	34.49797272027497	36.0	36.0	36.0	32.0	36.0
45	34.47244522189264	36.0	36.0	36.0	32.0	36.0
46	34.41565962280131	36.0	36.0	36.0	32.0	36.0
47	34.41104902079124	36.0	36.0	36.0	32.0	36.0
48	34.37335487586004	36.0	36.0	36.0	32.0	36.0
49	34.3417682944928	36.0	36.0	36.0	32.0	36.0
50	33.835161084144474	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	19.0
22	78.0
23	223.0
24	591.0
25	1363.0
26	2735.0
27	5412.0
28	9315.0
29	15093.0
30	22681.0
31	33928.0
32	50708.0
33	85016.0
34	185638.0
35	597909.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.643049463135924	21.589347223555325	12.540384784744768	25.227218528563977
2	12.56912459494892	23.243574838597965	43.32302669008336	20.86427387636975
3	17.75106830735987	28.45007128645082	30.58866481120716	23.210195594982146
4	10.742328180530162	18.69493980481087	40.93183814973999	29.63089386491899
5	10.867070209772498	40.62355918687185	36.36053324679385	12.148837356561797
6	28.243765756281736	40.28938130498621	19.688772622755536	11.778080315976522
7	25.7303252947916	33.23472317589348	24.093858401090714	16.941093128224203
8	23.239116109997486	40.118470704868045	21.68694605991408	14.95546712522039
9	26.705873273745095	15.144046683829451	21.587709282744676	36.56237075968078
10	13.594251192721185	31.166878068375425	35.86266738167276	19.376203357230633
11	33.00587505466432	22.933688461820058	26.7295199235392	17.330916559976412
12	22.763115975439145	26.125491484237873	33.819161503649894	17.292231036673083
13	28.61284201069739	23.70512330886878	27.011004102050627	20.671030578383203
14	19.253418870224415	24.50999986148406	28.9824816911609	27.254099577130624
15	20.637193112987454	35.58573444119701	23.896275306367578	19.880797139447953
16	19.34840122923003	29.989888336364196	30.986213706350163	19.67549672805561
17	19.164372908656652	31.315584824193586	29.74708968115609	19.772952585993664
18	19.34523515059651	29.044319164471847	33.474751512297246	18.135694172634395
19	22.415342025538383	27.825972530310256	28.92846047447646	20.830224969674905
20	22.889066541078883	29.141280322623413	29.853845895080113	18.115807241217592
21	21.81566694435815	28.297619306747507	27.589802852241093	22.29691089665326
22	21.949136946752493	31.1798392027814	28.32502567491892	18.545998175547187
23	19.724373066960585	30.832164192837936	29.537336971685363	19.906125768516116
24	21.623822367158265	28.742057594927946	30.51704042884535	19.11707960906844
25	20.501843251404452	29.805662135876222	30.04737245155405	19.645122161165276
26	18.35613239749326	30.95851051830686	30.979980489040422	19.705376595159464
27	19.695383659472412	30.5309909628243	29.75520275765449	20.0184226200488
28	18.7605000029682	30.940800265950607	28.976941053552242	21.321758677528955
29	20.394394457779352	29.35785988914767	28.560403833329705	21.68734181974327
30	19.325546099094304	29.717209814052243	29.92706146348027	21.030182623373182
31	21.46660677501252	28.58325896346543	29.31600828721082	20.634125974311228
32	19.7037935558427	30.40919587539106	29.739174484572295	20.147836084193944
33	19.055934715458577	30.55354927308813	30.968800273865803	19.42171573758749
34	19.90315756979719	30.385846045468845	30.978595329638253	18.732401055095703
35	19.529461351084482	30.73084967656528	30.29027004671945	19.44941892563079
36	18.690945212988048	30.21012868130846	31.32983217804443	19.76909392765906
37	19.28507965655962	29.685351147802447	28.9865382294101	22.043030966227835
38	19.68984302186375	31.052305597824905	29.051640721311866	20.20621065899948
39	21.24240883177635	28.435145847391052	29.7428352629923	20.5796100578403
40	22.050550402982445	28.371032755062263	30.2444608464907	19.333955995464592
41	21.301970686069453	27.506099648367393	31.37000180070722	19.821927864855933
42	20.200373201518925	30.088927233619007	30.617167665630436	19.093531899231632
43	18.646125412332275	30.022934282101566	31.108404553612594	20.22253575195357
44	18.27005463464442	29.791909481811867	30.817026379371416	21.121009504172296
45	18.680259697599915	30.09990956887903	30.354679958920126	20.865150774600927
46	18.970252712438928	28.20273588769919	30.82523839582711	22.00177300403477
47	20.839723205575464	28.198382529578097	30.37377537067855	20.588118894167888
48	18.480302043901638	29.229139004703608	30.25504742192153	22.035511529473222
49	20.102521583751685	28.329477972997307	30.30580362001516	21.26219682323585
50	18.43532084312841	30.78532757105882	29.455374033354705	21.32397755245807
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	3.0
2	3.0
3	18.5
4	34.0
5	49.0
6	64.0
7	95.5
8	127.0
9	200.5
10	274.0
11	405.5
12	537.0
13	847.5
14	1158.0
15	1693.0
16	2228.0
17	2760.5
18	3293.0
19	4088.0
20	4883.0
21	6056.5
22	7230.0
23	9078.0
24	10926.0
25	13870.5
26	16815.0
27	22063.5
28	27312.0
29	34102.5
30	40893.0
31	49032.0
32	57171.0
33	68355.5
34	79540.0
35	90354.0
36	101168.0
37	102115.5
38	103063.0
39	97318.5
40	91574.0
41	85488.5
42	79403.0
43	74443.0
44	69483.0
45	66385.5
46	63288.0
47	59726.5
48	56165.0
49	51984.0
50	47803.0
51	42582.5
52	37362.0
53	33228.5
54	29095.0
55	25965.0
56	22835.0
57	20299.5
58	17764.0
59	15199.0
60	12634.0
61	10551.5
62	8469.0
63	7056.5
64	5644.0
65	4697.5
66	3751.0
67	3251.0
68	2751.0
69	2488.5
70	2226.0
71	1691.5
72	1157.0
73	1068.5
74	980.0
75	727.5
76	475.0
77	404.0
78	333.0
79	311.0
80	289.0
81	249.0
82	209.0
83	174.0
84	139.0
85	109.5
86	80.0
87	68.5
88	57.0
89	35.5
90	14.0
91	8.5
92	3.0
93	3.5
94	4.0
95	3.5
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012169614747594275
2	0.0038586583346030628
3	2.9681987189254333E-4
4	1.9787991459502887E-4
5	0.0
6	5.936397437850867E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	1.9787991459502887E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1010714.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.25873521157341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.09631386539206	72.31016198061312
2	7.575346542358948	12.159754645581899
3	1.1317240451192248	2.7249222140938385
4	0.369296027941772	1.1855692848505799
5	0.1800260098385516	0.7224329927414211
6	0.12424832098753805	0.5983207856772838
7	0.0755770252804815	0.42460015220451885
8	0.06085171045478952	0.3907105053249828
9	0.04603383188280753	0.3325155411470702
>10	0.2989119081316353	4.504502872968477
>50	0.026051438689355912	1.467980994632494
>100	0.014131725443174476	2.1284043994277932
>500	9.91698986363777E-4	0.46860088117186915
>1k	4.958494931818885E-4	0.5815227495646657
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1735	0.17166082591118753	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	1416	0.14009897953328043	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	1377	0.13624032119867738	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	1317	0.1303039237608265	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	9.893995729751443E-5	0.0
11	0.0	0.0	0.0	9.893995729751443E-5	0.0
12	0.0	0.0	0.0	9.893995729751443E-5	0.0
13	0.0	0.0	0.0	9.893995729751443E-5	0.0
14	0.0	0.0	0.0	1.9787991459502887E-4	0.0
15	0.0	0.0	0.0	1.9787991459502887E-4	0.0
16	0.0	0.0	0.0	1.9787991459502887E-4	0.0
17	0.0	0.0	0.0	1.9787991459502887E-4	0.0
18	0.0	0.0	0.0	1.9787991459502887E-4	0.0
19	0.0	0.0	0.0	2.968198718925433E-4	0.0
20	0.0	0.0	0.0	6.92579701082601E-4	0.0
21	0.0	0.0	0.0	7.915196583801155E-4	0.0
22	0.0	0.0	0.0	0.0014840993594627165	0.0
23	0.0	0.0	0.0	0.0019787991459502886	0.0
24	0.0	0.0	0.0	0.0033639585481154907	0.0
25	0.0	0.0	0.0	0.003957598291900577	0.0
26	0.0	0.0	0.0	0.0056395775659583225	0.0
27	0.0	0.0	0.0	0.00801413654109867	0.0
28	0.0	0.0	0.0	0.01474205363732965	0.0
29	0.0	0.0	0.0	0.02701060834222144	0.0
30	0.0	0.0	0.0	0.05985867416499623	0.0
31	0.0	0.0	0.0	0.13683396094246245	0.0
32	0.0	0.0	0.0	0.24230395542161284	0.0
33	0.0	0.0	0.0	0.36370328302566307	0.0
34	0.0	0.0	0.0	0.5113216993135545	0.0
35	0.0	0.0	0.0	0.6642828732955118	0.0
36	0.0	0.0	0.0	0.8789825806311182	0.0
37	0.0	0.0	0.0	1.1951946841539742	0.0
38	0.0	0.0	0.0	1.5977813703975605	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAA	20	7.856421E-4	43.999554	38
AATGGCG	30	0.0057448572	29.333038	16
CGTAAAC	30	0.0057448572	29.333038	40
CCGACAC	55	1.5934755E-4	23.999758	36
TAGGACC	985	0.0	22.112545	4
ACCGGGG	50	0.0025802995	21.999777	38
CACCGCA	50	0.0025802995	21.999777	9
GTATCAA	2185	0.0	21.954866	1
GTAGGAC	2650	0.0	20.838554	3
GACGTGG	1235	0.0	20.485624	7
TGTAGGA	2780	0.0	20.260794	2
CGGAAGC	55	0.0044833743	19.999798	38
ATTGCGA	55	0.0044833743	19.999798	17
TGGTCGG	55	0.0044833743	19.999798	6
CTGTAGG	2740	0.0	19.756512	1
GTCCTAC	2360	0.0	19.487675	1
AGGACCT	1665	0.0	19.291096	5
GGACGTG	2575	0.0	19.052235	6
TAGGACG	2610	0.0	19.050558	4
GTCCTAA	1445	0.0	19.03566	1
>>END_MODULE