FastQCFastQC Report
Thu 2 Feb 2017
SRR4062702_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062702_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471854
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18270.3871960394528816No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14200.30094054516863267No Hit
TATCAACGCAGAGTACTTTTTTTTT12390.2625812221577013No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10770.22824856841311084No Hit
GTACATGGAAGCAGTGGTATCAACG10280.21786400030517913No Hit
TCCATGTACTCTGCGTTGATACCAC8750.1854387162130659No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8570.1816239769081114No Hit
GCGCAAGACGGACCAGAGCGAAAGC6950.14729132316352092No Hit
GGTATCAACGCAGAGTACTTTTTTT6270.13288008578924837No Hit
GTCTTGCGCCGGTCCAAGAATTTCA5710.12101200795161214No Hit
GAATAACGCCGCCGCATCGCCAGTC5530.11719726864665767No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG5510.11677340872388493No Hit
GATTAAGAGGGACGGCCGGGGGCAT5500.11656147876249857No Hit
GAACTACGACGGTATCTGATCGTCT5430.11507796903279405No Hit
GTATCTGATCGTCTTCGAACCTCCG5340.1131705993803168No Hit
GAATAGGACCGCGGTTCTATTTTGT4930.10448147096347599No Hit
GAGTACATGGAAGCAGTGGTATCAA4820.10215024138822601No Hit
GTCCTATTCCATTATTCCTAGCTGC4720.10003094177436239No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCGA402.7472049E-416.63196419
CCCAATT402.7516577E-416.62843513
AGTGGTC402.756117E-416.6249078
AAACCGA508.65457E-515.20798216
GGACTGA508.69384E-515.1999156
CCCCCGT508.7490815E-515.1886372
AACCGAC456.7231467E-414.78553917
AATAGAT456.7502557E-414.7776967
ATGGACT456.7665667E-414.7729934
TAGGGTC456.7665667E-414.7729934
ACCGACC400.005253550614.25748318
TCCAACG400.005253550614.25748318
TAGAACC759.666855E-713.92882254
CAAGACG1301.8189894E-1213.8801194
AATCCCC551.9556875E-413.81810511
GGGACTG551.9592114E-413.8151745
CGTCGTA852.6857379E-713.4116910
AAGACGG1351.8189894E-1213.367465
GACTGAT500.001496908313.2999267
TAGGACT500.001500503213.2956944
Produced by FastQC (version 0.11.5)