##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062702_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	471854
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98176130752309	32.0	32.0	32.0	32.0	32.0
2	30.676016310129828	32.0	32.0	32.0	32.0	32.0
3	30.65280362145918	32.0	32.0	32.0	32.0	32.0
4	30.701564466974954	32.0	32.0	32.0	32.0	32.0
5	30.52295201481814	32.0	32.0	32.0	32.0	32.0
6	34.12132354499485	36.0	36.0	36.0	32.0	36.0
7	33.97469768191008	36.0	36.0	36.0	32.0	36.0
8	33.946769551598585	36.0	36.0	36.0	32.0	36.0
9	34.10720053236806	36.0	36.0	36.0	32.0	36.0
10	33.79455933403129	36.0	36.0	36.0	32.0	36.0
11	34.13325944042013	36.0	36.0	36.0	32.0	36.0
12	33.896790108804844	36.0	36.0	36.0	32.0	36.0
13	34.049485645983715	36.0	36.0	36.0	32.0	36.0
14	33.951980909349075	36.0	36.0	36.0	32.0	36.0
15	33.90532028975064	36.0	36.0	36.0	32.0	36.0
16	33.88706464287682	36.0	36.0	36.0	32.0	36.0
17	33.80119698042191	36.0	36.0	36.0	32.0	36.0
18	33.837555684597355	36.0	36.0	36.0	32.0	36.0
19	33.81056004611596	36.0	36.0	36.0	32.0	36.0
20	33.773618534546706	36.0	36.0	36.0	32.0	36.0
21	33.754031967515374	36.0	36.0	36.0	32.0	36.0
22	33.72149647984334	36.0	36.0	36.0	27.0	36.0
23	33.715443760146144	36.0	36.0	36.0	27.0	36.0
24	33.68856892174274	36.0	36.0	36.0	27.0	36.0
25	33.232485048341225	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	18.0
5	39.0
6	82.0
7	26.0
8	72.0
9	84.0
10	47.0
11	24.0
12	28.0
13	22.0
14	131.0
15	167.0
16	191.0
17	305.0
18	418.0
19	598.0
20	919.0
21	1447.0
22	2003.0
23	2800.0
24	3764.0
25	5024.0
26	6857.0
27	8437.0
28	10922.0
29	14232.0
30	18159.0
31	24002.0
32	33913.0
33	47856.0
34	103795.0
35	185468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.27682460286341	17.410849591471234	10.743405784046713	25.56892002161864
2	17.099476728290792	20.523949906641885	35.76135079974313	26.61522256532419
3	19.706458962539077	24.33741323583956	27.87262226461082	28.083505537010545
4	12.662759519696474	14.96529139332535	35.34448954502581	37.027459541952375
5	14.604750163773165	36.55417022656878	33.038506303941794	15.802573305716267
6	34.01074961040613	34.869234275779455	16.860562499337426	14.25945361447699
7	29.811022252760655	30.531644388457323	21.046492640026127	18.6108407187559
8	28.713060299844734	31.29767268222737	20.080857959800102	19.908409058127795
9	27.529422246357747	13.911185968961334	18.859299296581334	39.70009248809958
10	16.237617040056186	26.21400215992345	31.130904515682783	26.41747628433758
11	37.4265320585452	21.463035812040893	21.71935140273375	19.391080726680162
12	24.57993268736976	23.887495384458678	28.461019366174767	23.0715525619968
13	29.415159635978622	19.163956027167927	25.661215682030846	25.759668654822605
14	23.727335419871626	19.65752479974537	24.996445811893267	31.618693968489737
15	25.420086053991664	26.406227457503416	23.117675014639364	25.05601147386556
16	25.79333843944065	25.55528917045558	23.810867521975002	24.840504868128768
17	23.59285680941448	25.491327762617928	25.525702669784454	25.390112758183136
18	24.815008944505866	24.489484104668758	26.648897905309195	24.04660904551618
19	25.224676540055558	25.027321940526235	25.7197607128533	24.028240806564906
20	25.32522783453749	23.975513754946583	25.654964618012453	25.044293792503474
21	26.940453226891435	24.035582491411013	24.453201426864695	24.570762854832857
22	25.51958195726184	24.27814614516033	25.164279589428375	25.037992308149455
23	24.304341182662434	23.824935060525288	25.863313016756933	26.00741074005535
24	24.256604190010524	25.315142100438017	25.495950901497405	24.932302808054054
25	24.64837450131568	24.466938290467702	25.59693574399457	25.28775146422205
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	156.0
1	156.0
2	319.5
3	483.0
4	483.0
5	483.0
6	1291.5
7	2100.0
8	2100.0
9	2100.0
10	2042.0
11	1984.0
12	1984.0
13	1984.0
14	1767.0
15	1550.0
16	1550.0
17	1550.0
18	2392.5
19	3235.0
20	3235.0
21	3235.0
22	5127.5
23	7020.0
24	7020.0
25	7020.0
26	10340.5
27	13661.0
28	13661.0
29	13661.0
30	16864.5
31	20068.0
32	20068.0
33	20068.0
34	23840.5
35	27613.0
36	27613.0
37	27613.0
38	30993.5
39	34374.0
40	34374.0
41	34374.0
42	39974.5
43	45575.0
44	45575.0
45	45575.0
46	54560.0
47	63545.0
48	63545.0
49	63545.0
50	63514.5
51	63484.0
52	63484.0
53	63484.0
54	58603.5
55	53723.0
56	53723.0
57	53723.0
58	49664.0
59	45605.0
60	45605.0
61	45605.0
62	41093.0
63	36581.0
64	36581.0
65	36581.0
66	30386.0
67	24191.0
68	24191.0
69	24191.0
70	18315.0
71	12439.0
72	12439.0
73	12439.0
74	9678.5
75	6918.0
76	6918.0
77	6918.0
78	5691.0
79	4464.0
80	4464.0
81	4464.0
82	3106.0
83	1748.0
84	1748.0
85	1748.0
86	1277.5
87	807.0
88	807.0
89	807.0
90	534.0
91	261.0
92	261.0
93	261.0
94	152.5
95	44.0
96	44.0
97	44.0
98	134.5
99	225.0
100	225.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00826526849406808
2	0.0031789494207954154
3	0.006145968880204469
4	0.01462316733565891
5	0.034968443628749575
6	0.04429336192974946
7	0.06718179775947644
8	0.08689128416840802
9	0.09367304293277157
10	0.11465410911002132
11	0.11868077837636218
12	0.13139657605954386
13	0.11931656826052126
14	0.12270744764270303
15	0.11062743984368045
16	0.11083936980506683
17	0.1229193776040894
18	0.13033692625261203
19	0.1316085060209302
20	0.12058814802883944
21	0.12948920640706657
22	0.1496225527387709
23	0.13690675505558925
24	0.13521131536449835
25	0.13012499629122568
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	471854.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.01894880322465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.95435902960038	43.730693758178504
2	11.341304881144158	12.252907354725762
3	3.2244914470372996	5.2255091518183105
4	1.3470619404739224	2.9106747998893305
5	0.7522101432527192	2.031680060881746
6	0.4678337495229704	1.5163132418341179
7	0.28558227300811373	1.0798797929313675
8	0.22226971397945258	0.9605421039970748
9	0.17474507473614256	0.8495590721208616
>10	0.9679604834028019	10.152821811268234
>50	0.14683725438130654	5.696787953279087
>100	0.10943902604125536	10.839399586784538
>500	0.003936655612994797	1.3516288113788457
>1k	0.0019683278064973986	1.4016025009122044
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1827	0.3871960394528816	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1420	0.30094054516863267	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1239	0.2625812221577013	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1077	0.22824856841311084	No Hit
GTACATGGAAGCAGTGGTATCAACG	1028	0.21786400030517913	No Hit
TCCATGTACTCTGCGTTGATACCAC	875	0.1854387162130659	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	857	0.1816239769081114	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	695	0.14729132316352092	No Hit
GGTATCAACGCAGAGTACTTTTTTT	627	0.13288008578924837	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	571	0.12101200795161214	No Hit
GAATAACGCCGCCGCATCGCCAGTC	553	0.11719726864665767	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	551	0.11677340872388493	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	550	0.11656147876249857	No Hit
GAACTACGACGGTATCTGATCGTCT	543	0.11507796903279405	No Hit
GTATCTGATCGTCTTCGAACCTCCG	534	0.1131705993803168	No Hit
GAATAGGACCGCGGTTCTATTTTGT	493	0.10448147096347599	No Hit
GAGTACATGGAAGCAGTGGTATCAA	482	0.10215024138822601	No Hit
GTCCTATTCCATTATTCCTAGCTGC	472	0.10003094177436239	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.1192996138636103E-4	0.0
5	0.0	0.0	0.0	2.1192996138636103E-4	0.0
6	0.0	0.0	0.0	2.1192996138636103E-4	0.0
7	0.0	0.0	0.0	2.1192996138636103E-4	0.0
8	0.0	0.0	0.0	2.1192996138636103E-4	0.0
9	0.0	0.0	0.0	2.1192996138636103E-4	0.0
10	0.0	0.0	0.0	2.1192996138636103E-4	0.0
11	0.0	0.0	0.0	2.1192996138636103E-4	0.0
12	0.0	0.0	0.0	2.1192996138636103E-4	4.2385992277272207E-4
13	0.0	0.0	0.0	2.1192996138636103E-4	6.357898841590831E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCCGA	40	2.7472049E-4	16.631964	19
CCCAATT	40	2.7516577E-4	16.628435	13
AGTGGTC	40	2.756117E-4	16.624907	8
AAACCGA	50	8.65457E-5	15.207982	16
GGACTGA	50	8.69384E-5	15.199915	6
CCCCCGT	50	8.7490815E-5	15.188637	2
AACCGAC	45	6.7231467E-4	14.785539	17
AATAGAT	45	6.7502557E-4	14.777696	7
ATGGACT	45	6.7665667E-4	14.772993	4
TAGGGTC	45	6.7665667E-4	14.772993	4
ACCGACC	40	0.0052535506	14.257483	18
TCCAACG	40	0.0052535506	14.257483	18
TAGAACC	75	9.666855E-7	13.9288225	4
CAAGACG	130	1.8189894E-12	13.880119	4
AATCCCC	55	1.9556875E-4	13.818105	11
GGGACTG	55	1.9592114E-4	13.815174	5
CGTCGTA	85	2.6857379E-7	13.41169	10
AAGACGG	135	1.8189894E-12	13.36746	5
GACTGAT	50	0.0014969083	13.299926	7
TAGGACT	50	0.0015005032	13.295694	4
>>END_MODULE