FastQCFastQC Report
Thu 2 Feb 2017
SRR4062702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471854
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT6450.13669482509420286No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC6130.12991306632983932No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA5610.11889270833774855No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA5460.11571375891695312No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT5430.11507796903279405No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA5350.11338252934170315No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA5060.10723656046149868No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC5050.10702463050011232No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4790.10151445150406693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCGG353.2150044E-431.428425
ACCGCGC500.002578200821.9998938
GACGGAC2300.021.0433757
GTGCTAC755.454867E-520.5375881
GCATATA654.919161E-420.3118971
TAACGGC654.926199E-420.30759236
ATTCGTG550.00447975719.99990344
TATAACA550.00447975719.9999034
GTATTAG1152.0105108E-719.1343981
GGCGTTA1152.0150037E-719.13034242
AAGACGG2550.018.9802995
ATACACT1658.185452E-1118.6665784
ATCGTTT1557.166818E-1018.45152529
CATCGTT1557.166818E-1018.45152528
CATATAG600.0074057818.3332442
GCCCTAG851.4285452E-418.117567
GCGGGGT1102.7892293E-617.99991244
AGACGGA2700.017.9258386
CAAGACG2700.017.9258384
GTTCAAA2100.017.8132131
Produced by FastQC (version 0.11.5)