##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062702_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	471854
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18796280205318	32.0	32.0	32.0	32.0	32.0
2	31.37404790464847	32.0	32.0	32.0	32.0	32.0
3	31.473354045954892	32.0	32.0	32.0	32.0	32.0
4	31.571729814730826	32.0	32.0	32.0	32.0	32.0
5	31.510715178847693	32.0	32.0	32.0	32.0	32.0
6	35.06800408600966	36.0	36.0	36.0	36.0	36.0
7	35.0918737575606	36.0	36.0	36.0	36.0	36.0
8	35.031357157086724	36.0	36.0	36.0	36.0	36.0
9	35.13499090820466	36.0	36.0	36.0	36.0	36.0
10	35.03090362696936	36.0	36.0	36.0	36.0	36.0
11	35.16151394287216	36.0	36.0	36.0	36.0	36.0
12	35.06639553760273	36.0	36.0	36.0	36.0	36.0
13	35.11163622645988	36.0	36.0	36.0	36.0	36.0
14	35.070104311927	36.0	36.0	36.0	36.0	36.0
15	35.032206996231885	36.0	36.0	36.0	36.0	36.0
16	35.047709672907295	36.0	36.0	36.0	36.0	36.0
17	35.00830341588712	36.0	36.0	36.0	36.0	36.0
18	35.032902126505235	36.0	36.0	36.0	36.0	36.0
19	35.000008477198456	36.0	36.0	36.0	36.0	36.0
20	34.980184548610374	36.0	36.0	36.0	36.0	36.0
21	34.967619221199776	36.0	36.0	36.0	36.0	36.0
22	34.966364595828374	36.0	36.0	36.0	36.0	36.0
23	34.92441941787078	36.0	36.0	36.0	32.0	36.0
24	34.91578962984313	36.0	36.0	36.0	32.0	36.0
25	34.88187447812247	36.0	36.0	36.0	32.0	36.0
26	34.84421876258334	36.0	36.0	36.0	32.0	36.0
27	34.8307145854438	36.0	36.0	36.0	32.0	36.0
28	34.81072323218623	36.0	36.0	36.0	32.0	36.0
29	34.777359098365174	36.0	36.0	36.0	32.0	36.0
30	34.757882734913764	36.0	36.0	36.0	32.0	36.0
31	34.76532147655843	36.0	36.0	36.0	32.0	36.0
32	34.719870977039506	36.0	36.0	36.0	32.0	36.0
33	34.68126581527337	36.0	36.0	36.0	32.0	36.0
34	34.64970096682448	36.0	36.0	36.0	32.0	36.0
35	34.604097877733366	36.0	36.0	36.0	32.0	36.0
36	34.57335531753466	36.0	36.0	36.0	32.0	36.0
37	34.57232533792232	36.0	36.0	36.0	32.0	36.0
38	34.52456480182429	36.0	36.0	36.0	32.0	36.0
39	34.507847766470135	36.0	36.0	36.0	32.0	36.0
40	34.47872858977565	36.0	36.0	36.0	32.0	36.0
41	34.44310104396699	36.0	36.0	36.0	32.0	36.0
42	34.38591174388688	36.0	36.0	36.0	32.0	36.0
43	34.380901719599706	36.0	36.0	36.0	32.0	36.0
44	34.335171472531755	36.0	36.0	36.0	32.0	36.0
45	34.25431383436402	36.0	36.0	36.0	32.0	36.0
46	34.263888830019454	36.0	36.0	36.0	32.0	36.0
47	34.218211565441855	36.0	36.0	36.0	32.0	36.0
48	34.16428810606671	36.0	36.0	36.0	32.0	36.0
49	34.125587575817946	36.0	36.0	36.0	32.0	36.0
50	33.58955100518381	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	6.0
21	15.0
22	52.0
23	122.0
24	367.0
25	899.0
26	1787.0
27	3308.0
28	5606.0
29	8524.0
30	12170.0
31	16889.0
32	24546.0
33	39098.0
34	82293.0
35	276169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.89811058403598	17.94563636903172	11.296859169199596	25.8593938777327
2	16.56818528628834	21.172366435510945	35.559902677224095	26.69954560097662
3	18.947002562238662	24.846297469762828	28.313902846861204	27.892797121137303
4	12.412128353533834	15.505464625635526	35.48817110413624	36.594235916694394
5	14.39089209797946	36.952955787171454	32.85105138453844	15.805100730310647
6	34.23517169614985	35.03415273041702	16.665006538080878	14.065669035352263
7	30.008434812463175	30.538471645890464	20.950548262810106	18.502545278836248
8	28.873761799200597	31.46079083784391	19.86059247140005	19.80485489155544
9	27.790587766554907	13.76124818270058	18.714687170183996	39.73347688056052
10	16.264988746519048	26.278467492063225	31.091184985186093	26.36535877623163
11	37.91278658229029	21.18494280010342	21.71031717438021	19.19195344322608
12	24.81381952892208	23.840849076197298	28.293285634963354	23.052045759917263
13	29.62441772243109	19.026860003306105	25.879615304734095	25.46910696952871
14	23.95253616584791	19.448388696503578	25.129171311464987	31.469903826183522
15	25.59944389578132	26.219550962797815	23.03890610231131	25.142099039109556
16	26.017793639558	25.491571545435665	23.71199565967439	24.77863915533195
17	23.812450461371526	25.340677412928574	25.608133024198164	25.238739101501732
18	25.01027860312724	24.04536148893514	26.770145002479577	24.174214905458044
19	25.4926311952426	24.815726898574557	25.750761888211184	23.94088001797166
20	25.52569226921887	23.797403434113093	25.72172748350125	24.955176813166783
21	27.05688624023533	23.87285050036664	24.591504999427787	24.478758259970245
22	25.82451351477364	23.90887859380232	25.25293840891462	25.01366948250942
23	24.526654431243564	23.541180110796983	25.951247631682683	25.980917826276773
24	24.605704306840675	25.014729132316354	25.350426191152348	25.029140369690623
25	25.14824500798976	24.063375535652977	25.58100598914071	25.207373467216556
26	24.35795818198002	24.861291840272624	26.293938379244423	24.48681159850293
27	25.146337638337286	24.654024338036766	25.52696384898719	24.672674174638765
28	24.397165224836495	24.114238726385704	26.38973072179106	25.098865326986736
29	24.19074544244618	24.55653655579904	26.304111017390973	24.948606984363806
30	24.313452890088882	24.9424610154836	26.273805032912723	24.47028106151479
31	24.371521699508747	24.723961225294264	25.381579895476143	25.522937179720845
32	24.288233224683907	24.902194322820197	25.396838852695964	25.412733599799935
33	24.104278018200546	24.317479559355224	25.877496005120225	25.700746417324005
34	24.87867009710631	24.54085373865645	26.128633009363067	24.45184315487418
35	25.820910705430066	24.386992586689953	25.7738622540023	24.01823445387768
36	24.421961030318702	25.20419451779576	25.242765770768077	25.131078681117465
37	25.409342720417754	24.933771887066765	25.40722342080389	24.24966197171159
38	24.533012330085153	24.652328898345672	25.62169654172689	25.19296222984228
39	24.96598524119749	24.606340096724836	25.24361349061362	25.184061171464055
40	25.45745082165246	24.80279917092999	25.476524518177236	24.263225489240316
41	23.83957749642898	25.176431692854145	26.26193695507509	24.722053855641786
42	25.453636082347508	25.522937179720845	25.382215685360304	23.641211052571347
43	24.62223484382881	24.198162991094705	25.677222191610117	25.502379973466372
44	24.203249310167976	24.849847622357764	25.57146914087832	25.375433926595935
45	24.25432443086209	25.095050587681783	25.672135872536845	24.978489108919284
46	24.09606381648522	24.826164080762442	25.584451089640204	25.493321013112137
47	24.513514773637606	24.542973038270315	26.118884231139294	24.824627956952785
48	25.076824611002557	25.658148495085342	24.737312812861607	24.527714081050494
49	23.86522102175673	25.545189825666416	25.377129366287026	25.212459786289827
50	23.835762757124026	25.79166437075875	24.90071081309049	25.471862059026734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	33.5
2	34.0
3	105.0
4	176.0
5	195.5
6	215.0
7	178.0
8	141.0
9	201.0
10	261.0
11	374.5
12	488.0
13	821.0
14	1154.0
15	1547.0
16	1940.0
17	2201.0
18	2462.0
19	2576.0
20	2690.0
21	2948.0
22	3206.0
23	3272.0
24	3338.0
25	3490.5
26	3643.0
27	4375.5
28	5108.0
29	5650.5
30	6193.0
31	6417.0
32	6641.0
33	7540.5
34	8440.0
35	9619.5
36	10799.0
37	13444.5
38	16090.0
39	17391.0
40	18692.0
41	20813.0
42	22934.0
43	23475.5
44	24017.0
45	27056.5
46	30096.0
47	32603.5
48	35111.0
49	35726.0
50	36341.0
51	34537.0
52	32733.0
53	32331.5
54	31930.0
55	32810.0
56	33690.0
57	33231.5
58	32773.0
59	29927.0
60	27081.0
61	23943.0
62	20805.0
63	18288.0
64	15771.0
65	13521.0
66	11271.0
67	9744.5
68	8218.0
69	7366.0
70	6514.0
71	5025.5
72	3537.0
73	3254.5
74	2972.0
75	2118.0
76	1264.0
77	1177.5
78	1091.0
79	936.0
80	781.0
81	613.0
82	445.0
83	404.0
84	363.0
85	298.5
86	234.0
87	168.5
88	103.0
89	62.0
90	21.0
91	14.0
92	7.0
93	5.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014411237374272549
2	0.004662459150499942
3	2.11929961386361E-4
4	2.11929961386361E-4
5	0.0
6	6.357898841590831E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.11929961386361E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	471854.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.85621467342747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.60736212227681	47.214930618785814
2	11.238930136044237	13.004839093012578
3	3.0425061237116315	5.280836623160336
4	1.2914193428030947	2.9886653892252992
5	0.6889970702809642	1.9931381203769034
6	0.40129203578231204	1.3930342901374912
7	0.2797198624334103	1.1328472686558293
8	0.20185644674817674	0.9342919933022236
9	0.14544849580306415	0.7573589457398276
>10	0.8837835292317452	9.85767698183103
>50	0.12333235640526867	5.0097903916129
>100	0.09240722817876308	9.483882529292424
>500	0.002945250300518345	0.9487077548673432
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	645	0.13669482509420286	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	613	0.12991306632983932	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	561	0.11889270833774855	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	546	0.11571375891695312	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	543	0.11507796903279405	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	535	0.11338252934170315	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	506	0.10723656046149868	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	505	0.10702463050011232	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	479	0.10151445150406693	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.357898841590831E-4
2	0.0	0.0	0.0	0.0	6.357898841590831E-4
3	0.0	0.0	0.0	0.0	6.357898841590831E-4
4	0.0	0.0	0.0	0.0	6.357898841590831E-4
5	0.0	0.0	0.0	0.0	6.357898841590831E-4
6	0.0	0.0	0.0	0.0	8.477198455454441E-4
7	0.0	0.0	0.0	0.0	8.477198455454441E-4
8	2.1192996138636103E-4	0.0	0.0	0.0	8.477198455454441E-4
9	2.1192996138636103E-4	0.0	0.0	0.0	8.477198455454441E-4
10	2.1192996138636103E-4	0.0	0.0	0.0	8.477198455454441E-4
11	2.1192996138636103E-4	0.0	0.0	0.0	8.477198455454441E-4
12	2.1192996138636103E-4	0.0	0.0	0.0	0.0010596498069318052
13	2.1192996138636103E-4	0.0	0.0	0.0	0.0010596498069318052
14	2.1192996138636103E-4	0.0	0.0	2.1192996138636103E-4	0.0010596498069318052
15	2.1192996138636103E-4	0.0	0.0	2.1192996138636103E-4	0.0010596498069318052
16	2.1192996138636103E-4	0.0	0.0	2.1192996138636103E-4	0.0010596498069318052
17	2.1192996138636103E-4	0.0	0.0	4.2385992277272207E-4	0.0010596498069318052
18	2.1192996138636103E-4	0.0	0.0	4.2385992277272207E-4	0.0010596498069318052
19	2.1192996138636103E-4	0.0	0.0	4.2385992277272207E-4	0.0010596498069318052
20	2.1192996138636103E-4	0.0	0.0	8.477198455454441E-4	0.0010596498069318052
21	2.1192996138636103E-4	0.0	0.0	0.0010596498069318052	0.0010596498069318052
22	2.1192996138636103E-4	0.0	0.0	0.0019073696524772492	0.0010596498069318052
23	2.1192996138636103E-4	0.0	0.0	0.004238599227727221	0.0012715797683181661
24	2.1192996138636103E-4	0.0	0.0	0.009324918300999886	0.0012715797683181661
25	2.1192996138636103E-4	0.0	0.0	0.011656147876249858	0.0012715797683181661
26	4.2385992277272207E-4	0.0	0.0	0.014623167335658912	0.0012715797683181661
27	4.2385992277272207E-4	0.0	0.0	0.019285626486158853	0.0012715797683181661
28	4.2385992277272207E-4	0.0	0.0	0.029882124555476907	0.0012715797683181661
29	4.2385992277272207E-4	0.0	0.0	0.056161439767385676	0.0012715797683181661
30	4.2385992277272207E-4	0.0	0.0	0.09282532308722613	0.0012715797683181661
31	8.477198455454441E-4	0.0	0.0	0.15852361111699806	0.0012715797683181661
32	8.477198455454441E-4	0.0	0.0	0.23269909760222443	0.0012715797683181661
33	8.477198455454441E-4	0.0	0.0	0.31747108215676884	0.0012715797683181661
34	8.477198455454441E-4	0.0	0.0	0.4117799149736995	0.0014835097297045273
35	8.477198455454441E-4	0.0	0.0	0.5185926155124254	0.0014835097297045273
36	8.477198455454441E-4	0.0	0.0	0.6665197285601054	0.0014835097297045273
37	8.477198455454441E-4	0.0	0.0	0.8898939078613299	0.0014835097297045273
38	8.477198455454441E-4	0.0	0.0	1.1480246008299178	0.0014835097297045273
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGCGG	35	3.2150044E-4	31.42842	5
ACCGCGC	50	0.0025782008	21.999893	8
GACGGAC	230	0.0	21.043375	7
GTGCTAC	75	5.454867E-5	20.537588	1
GCATATA	65	4.919161E-4	20.311897	1
TAACGGC	65	4.926199E-4	20.307592	36
ATTCGTG	55	0.004479757	19.999903	44
TATAACA	55	0.004479757	19.999903	4
GTATTAG	115	2.0105108E-7	19.134398	1
GGCGTTA	115	2.0150037E-7	19.130342	42
AAGACGG	255	0.0	18.980299	5
ATACACT	165	8.185452E-11	18.666578	4
ATCGTTT	155	7.166818E-10	18.451525	29
CATCGTT	155	7.166818E-10	18.451525	28
CATATAG	60	0.00740578	18.333244	2
GCCCTAG	85	1.4285452E-4	18.11756	7
GCGGGGT	110	2.7892293E-6	17.999912	44
AGACGGA	270	0.0	17.925838	6
CAAGACG	270	0.0	17.925838	4
GTTCAAA	210	0.0	17.813213	1
>>END_MODULE