FastQCFastQC Report
Thu 2 Feb 2017
SRR4062701_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062701_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1937837
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88060.454424185315896No Hit
GTACATGGAAGCAGTGGTATCAACG58010.2993543832634014No Hit
TATCAACGCAGAGTACTTTTTTTTT55230.2850084914262655No Hit
TCCATGTACTCTGCGTTGATACCAC50370.2599289826750134No Hit
GTACTTTTTTTTTTTTTTTTTTTTT46080.23779089778964896No Hit
GAGTACTTTTTTTTTTTTTTTTTTT32370.1670419132259318No Hit
GGTATCAACGCAGAGTACTTTTTTT31850.16435850899740279No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30040.1550181981250229No Hit
GTACATGGGGTGGTATCAACGCAAA26840.13850494133407504No Hit
GAGTACATGGAAGCAGTGGTATCAA23470.1211144177761081No Hit
CATGTACTCTGCGTTGATACCACTG21040.1085746634004821No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG20170.10408512171044314No Hit
GTCTTGCGCCGGTCCAAGAATTTCA19610.10119530177202726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1600.013.6476591
CGAACGA957.368908E-813.00289916
TAGGACG905.423153E-712.6636024
CGTTATT1800.012.6603332
GTCTTAA853.9725783E-612.2863841
GCCTTAC701.0975964E-412.2066021
CCGTCGT2900.012.1215139
CGTCGTA2900.012.12151310
CGCATCG2450.012.02246713
GTATTAC1353.7471182E-1011.9554381
ATACCGT3350.011.9097196
TAACGAA1052.7149508E-711.7639213
CCGATAA1052.718607E-711.7627059
AGGACGT1302.6248017E-911.6900835
AGGCCCG4000.011.6382910
CGAGCCG3850.011.59938915
ACGAACG1157.05968E-811.56719815
ATTACAC1657.2759576E-1211.5102843
AAGACGG4150.011.4436045
CGCCAGT2750.011.40372118
Produced by FastQC (version 0.11.5)