##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062701_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1937837
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.989507373427177	32.0	32.0	32.0	32.0	32.0
2	30.67990651432499	32.0	32.0	32.0	32.0	32.0
3	30.692383311909104	32.0	32.0	32.0	32.0	32.0
4	30.72005488593726	32.0	32.0	32.0	32.0	32.0
5	30.554537868768115	32.0	32.0	32.0	32.0	32.0
6	34.15708338730244	36.0	36.0	36.0	32.0	36.0
7	33.992158267181395	36.0	36.0	36.0	32.0	36.0
8	33.98452449819051	36.0	36.0	36.0	32.0	36.0
9	34.119315504864446	36.0	36.0	36.0	32.0	36.0
10	33.83255247990414	36.0	36.0	36.0	32.0	36.0
11	34.1718880380548	36.0	36.0	36.0	32.0	36.0
12	33.93772180013077	36.0	36.0	36.0	32.0	36.0
13	34.06498069755093	36.0	36.0	36.0	32.0	36.0
14	33.968354407517246	36.0	36.0	36.0	32.0	36.0
15	33.93940150796997	36.0	36.0	36.0	32.0	36.0
16	33.9173532139184	36.0	36.0	36.0	32.0	36.0
17	33.831973483837906	36.0	36.0	36.0	32.0	36.0
18	33.86207869908563	36.0	36.0	36.0	32.0	36.0
19	33.850609210165764	36.0	36.0	36.0	32.0	36.0
20	33.7987942226307	36.0	36.0	36.0	32.0	36.0
21	33.75878879389752	36.0	36.0	36.0	32.0	36.0
22	33.73225766666649	36.0	36.0	36.0	32.0	36.0
23	33.714080183214584	36.0	36.0	36.0	27.0	36.0
24	33.694837594699656	36.0	36.0	36.0	27.0	36.0
25	33.22519283097598	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	12.0
4	64.0
5	171.0
6	329.0
7	95.0
8	257.0
9	384.0
10	222.0
11	65.0
12	142.0
13	89.0
14	729.0
15	795.0
16	1069.0
17	1453.0
18	1977.0
19	2809.0
20	4291.0
21	6120.0
22	8640.0
23	11875.0
24	15639.0
25	20419.0
26	26893.0
27	33492.0
28	43479.0
29	55993.0
30	71254.0
31	95175.0
32	135588.0
33	193139.0
34	426087.0
35	779089.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.95518616822256	17.846933683166956	12.371476655366228	24.82640349324425
2	16.017294123961175	20.287619570557467	38.20395424013607	25.491132065345283
3	18.933254341130716	24.814423370682608	28.31916909560809	27.933153192578587
4	12.586248630369942	16.043109735044002	35.749060548615866	35.62158108597019
5	14.506669777732581	36.887037850556304	32.89740554654184	15.70888682516928
6	33.16470112656089	35.377605181736094	17.37808325120279	14.079610440500229
7	29.748678068327344	31.354876688561163	20.52371214937828	18.372733093733217
8	28.103643117454073	32.51782925236966	19.614605112016463	19.763922518159806
9	27.480835594425855	15.235915498414007	19.048858709997	38.23439019716314
10	16.338306864092417	26.82591264633857	30.897353764763018	25.938426724806003
11	36.142230378329	22.198749305112102	22.202624144693743	19.45639617186515
12	25.13452569551877	24.27053331803869	28.133412700135068	22.46152828630747
13	29.245881549403325	20.04884379594928	25.40636860274918	25.29890605189821
14	23.961366035322158	20.17939559772441	25.43462951931259	30.424608847640837
15	25.04924659045177	26.978917344770565	23.457495529964607	24.514340534813066
16	25.2082832869678	26.534673413562498	24.13446263764365	24.122580661826053
17	23.44469203835461	26.27050033660257	25.598793500651247	24.68601412439157
18	24.380677499675773	25.156752081373124	26.98869111559946	23.473879303351637
19	24.810603124803006	25.86922082471734	26.281290141235143	23.03888590924451
20	25.37648330391869	24.16736586716706	26.29162654013549	24.164524288778765
21	26.56859857703099	24.524256093087182	24.987108541430928	23.920036788450908
22	25.259920062759107	24.767986654387713	25.43785122412298	24.5342420587302
23	23.617436867316723	24.85554539300644	26.046062184465768	25.48095555521106
24	23.79607190653597	25.977398736106732	25.892553570782674	24.333975786574623
25	24.357397108874732	25.095343476780418	25.75893717535294	24.788322238991903
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	724.0
1	724.0
2	1349.0
3	1974.0
4	1974.0
5	1974.0
6	4400.0
7	6826.0
8	6826.0
9	6826.0
10	7022.5
11	7219.0
12	7219.0
13	7219.0
14	7123.0
15	7027.0
16	7027.0
17	7027.0
18	11582.0
19	16137.0
20	16137.0
21	16137.0
22	24976.5
23	33816.0
24	33816.0
25	33816.0
26	48527.0
27	63238.0
28	63238.0
29	63238.0
30	76940.0
31	90642.0
32	90642.0
33	90642.0
34	107451.5
35	124261.0
36	124261.0
37	124261.0
38	140808.0
39	157355.0
40	157355.0
41	157355.0
42	179666.5
43	201978.0
44	201978.0
45	201978.0
46	237545.5
47	273113.0
48	273113.0
49	273113.0
50	266242.0
51	259371.0
52	259371.0
53	259371.0
54	233901.5
55	208432.0
56	208432.0
57	208432.0
58	190473.5
59	172515.0
60	172515.0
61	172515.0
62	151345.5
63	130176.0
64	130176.0
65	130176.0
66	106980.0
67	83784.0
68	83784.0
69	83784.0
70	64193.0
71	44602.0
72	44602.0
73	44602.0
74	35203.5
75	25805.0
76	25805.0
77	25805.0
78	21246.0
79	16687.0
80	16687.0
81	16687.0
82	11661.0
83	6635.0
84	6635.0
85	6635.0
86	4963.0
87	3291.0
88	3291.0
89	3291.0
90	2190.0
91	1089.0
92	1089.0
93	1089.0
94	661.0
95	233.0
96	233.0
97	233.0
98	570.0
99	907.0
100	907.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007947004830643652
2	0.003560670995548129
3	0.004799165254869217
4	0.013933060417362244
5	0.0343682156961602
6	0.04541145617510657
7	0.06589821538137625
8	0.08736544920960844
9	0.09422877156334615
10	0.11647006430365403
11	0.11729572714320141
12	0.12999029330124257
13	0.11760535070803169
14	0.1206499824288627
15	0.11198052261361507
16	0.11001957336969001
17	0.11972111173437189
18	0.1267392458705247
19	0.12813255191226092
20	0.11832780569263565
21	0.12627481052327932
22	0.14547147154275616
23	0.13556351746818746
24	0.1314352032704505
25	0.12725528514524181
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1937837.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.68551368039224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.33414823995564	36.67681704737058
2	14.449920406796377	14.07003595291328
3	4.5838396764236355	6.694997678257429
4	2.000797220159982	3.896393617351583
5	0.9739048108535441	2.3707527996105022
6	0.6160407855664158	1.799535725602399
7	0.38097880550400354	1.298370419311326
8	0.27242689278194987	1.0610594572353897
9	0.20645003879287493	0.904601356917122
>10	0.9873164032082542	8.686554401436497
>50	0.08421730477097697	2.875634439459078
>100	0.09120757168004891	9.655966823935598
>500	0.012465714149132777	4.179671526880171
>1k	0.005859951095746176	4.524151944493277
>5k	4.2617826150881285E-4	1.3054568092258743
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8806	0.454424185315896	No Hit
GTACATGGAAGCAGTGGTATCAACG	5801	0.2993543832634014	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5523	0.2850084914262655	No Hit
TCCATGTACTCTGCGTTGATACCAC	5037	0.2599289826750134	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4608	0.23779089778964896	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3237	0.1670419132259318	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3185	0.16435850899740279	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3004	0.1550181981250229	No Hit
GTACATGGGGTGGTATCAACGCAAA	2684	0.13850494133407504	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2347	0.1211144177761081	No Hit
CATGTACTCTGCGTTGATACCACTG	2104	0.1085746634004821	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2017	0.10408512171044314	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1961	0.10119530177202726	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.1603927471712014E-5	0.0
5	0.0	0.0	0.0	5.1603927471712014E-5	5.1603927471712014E-5
6	0.0	0.0	0.0	5.1603927471712014E-5	2.0641570988684806E-4
7	0.0	0.0	0.0	1.0320785494342403E-4	2.0641570988684806E-4
8	0.0	0.0	0.0	1.5481178241513605E-4	2.0641570988684806E-4
9	0.0	0.0	0.0	2.0641570988684806E-4	2.0641570988684806E-4
10	0.0	0.0	0.0	2.0641570988684806E-4	2.0641570988684806E-4
11	0.0	0.0	0.0	2.0641570988684806E-4	2.0641570988684806E-4
12	0.0	0.0	0.0	3.096235648302721E-4	3.096235648302721E-4
13	0.0	0.0	0.0	3.096235648302721E-4	3.096235648302721E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	160	0.0	13.647659	1
CGAACGA	95	7.368908E-8	13.002899	16
TAGGACG	90	5.423153E-7	12.663602	4
CGTTATT	180	0.0	12.660333	2
GTCTTAA	85	3.9725783E-6	12.286384	1
GCCTTAC	70	1.0975964E-4	12.206602	1
CCGTCGT	290	0.0	12.121513	9
CGTCGTA	290	0.0	12.121513	10
CGCATCG	245	0.0	12.022467	13
GTATTAC	135	3.7471182E-10	11.955438	1
ATACCGT	335	0.0	11.909719	6
TAACGAA	105	2.7149508E-7	11.76392	13
CCGATAA	105	2.718607E-7	11.762705	9
AGGACGT	130	2.6248017E-9	11.690083	5
AGGCCCG	400	0.0	11.63829	10
CGAGCCG	385	0.0	11.599389	15
ACGAACG	115	7.05968E-8	11.567198	15
ATTACAC	165	7.2759576E-12	11.510284	3
AAGACGG	415	0.0	11.443604	5
CGCCAGT	275	0.0	11.403721	18
>>END_MODULE