FastQCFastQC Report
Thu 2 Feb 2017
SRR4062701_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062701_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1937837
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25190.12999029330124257No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA24930.12864859118697805No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA21530.11110325584659597No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21320.11001957336969001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG5450.018.5720311
TAGGACG1456.3118932E-918.2072374
ATACCGT6050.018.182166
AATACCG851.4311905E-418.1179875
CTAGCGG6000.017.59987629
GTCGTAG5300.017.4338411
GGTTGCG902.2163584E-417.110997
CGCAATA6200.017.03213936
CGTCGTA5450.016.9540110
ACCGTCG5600.016.8927368
CCGTCGT5650.016.7432449
CGGTCCA6600.016.6665510
CGAATGC6650.016.54123743
TACCGTC5750.016.4520597
TCTAGCG6450.016.37197928
GTGTACG1105.615706E-516.0027771
TAGCGGC6600.015.999886530
TATTAGC4550.015.9563432
CAATACG6800.015.8528338
CGCCGGT7000.015.7141767
Produced by FastQC (version 0.11.5)