##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062701_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1937837
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.164814171676976	32.0	32.0	32.0	32.0	32.0
2	31.3796702199411	32.0	32.0	32.0	32.0	32.0
3	31.468150313984097	32.0	32.0	32.0	32.0	32.0
4	31.584314366997845	32.0	32.0	32.0	32.0	32.0
5	31.51304469880594	32.0	32.0	32.0	32.0	32.0
6	35.08031738479552	36.0	36.0	36.0	36.0	36.0
7	35.09245308041904	36.0	36.0	36.0	36.0	36.0
8	35.04669845812625	36.0	36.0	36.0	36.0	36.0
9	35.147690956463315	36.0	36.0	36.0	36.0	36.0
10	35.04471119087932	36.0	36.0	36.0	36.0	36.0
11	35.16845224856373	36.0	36.0	36.0	36.0	36.0
12	35.07694042378177	36.0	36.0	36.0	36.0	36.0
13	35.11411640917167	36.0	36.0	36.0	36.0	36.0
14	35.07244159338479	36.0	36.0	36.0	36.0	36.0
15	35.04191270989252	36.0	36.0	36.0	36.0	36.0
16	35.05231348147445	36.0	36.0	36.0	36.0	36.0
17	35.02851736239942	36.0	36.0	36.0	36.0	36.0
18	35.0374902533082	36.0	36.0	36.0	36.0	36.0
19	35.00920356046458	36.0	36.0	36.0	36.0	36.0
20	34.98989801515814	36.0	36.0	36.0	36.0	36.0
21	34.97062446428673	36.0	36.0	36.0	36.0	36.0
22	34.96778831243288	36.0	36.0	36.0	36.0	36.0
23	34.92350491811231	36.0	36.0	36.0	32.0	36.0
24	34.90496362697172	36.0	36.0	36.0	32.0	36.0
25	34.87760993313679	36.0	36.0	36.0	32.0	36.0
26	34.83205140576839	36.0	36.0	36.0	32.0	36.0
27	34.814650561424926	36.0	36.0	36.0	32.0	36.0
28	34.7898584865497	36.0	36.0	36.0	32.0	36.0
29	34.7628918221708	36.0	36.0	36.0	32.0	36.0
30	34.745957477331686	36.0	36.0	36.0	32.0	36.0
31	34.73579047154121	36.0	36.0	36.0	32.0	36.0
32	34.69107463630842	36.0	36.0	36.0	32.0	36.0
33	34.65224526108233	36.0	36.0	36.0	32.0	36.0
34	34.6306268277466	36.0	36.0	36.0	32.0	36.0
35	34.584279276327166	36.0	36.0	36.0	32.0	36.0
36	34.553372136046534	36.0	36.0	36.0	32.0	36.0
37	34.53887814093755	36.0	36.0	36.0	32.0	36.0
38	34.493893449242634	36.0	36.0	36.0	32.0	36.0
39	34.45790899853806	36.0	36.0	36.0	32.0	36.0
40	34.422224366652095	36.0	36.0	36.0	32.0	36.0
41	34.385071603029566	36.0	36.0	36.0	32.0	36.0
42	34.31934987308014	36.0	36.0	36.0	32.0	36.0
43	34.313343175922434	36.0	36.0	36.0	32.0	36.0
44	34.27060583526891	36.0	36.0	36.0	32.0	36.0
45	34.17911310394012	36.0	36.0	36.0	32.0	36.0
46	34.17674241951207	36.0	36.0	36.0	32.0	36.0
47	34.094229803641895	36.0	36.0	36.0	32.0	36.0
48	34.04205978108582	36.0	36.0	36.0	32.0	36.0
49	34.02342663495433	36.0	36.0	36.0	32.0	36.0
50	33.49604430093966	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	8.0
20	27.0
21	83.0
22	255.0
23	673.0
24	1875.0
25	4121.0
26	8465.0
27	15277.0
28	25046.0
29	36562.0
30	50574.0
31	70507.0
32	101320.0
33	155297.0
34	327333.0
35	1140413.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.31959232189373	18.543294508567442	12.958959158383266	25.178154011155563
2	15.494095256181867	20.681156219229997	37.8183308184072	26.00641770618094
3	18.349841523439153	24.952137085579405	28.728895163962086	27.969126227019352
4	12.35410894540448	16.394653202830174	35.907634971641	35.34360288012435
5	14.193608647166919	36.88106894439522	32.980637690373335	15.944684718064522
6	33.38894545281906	35.5960052161519	17.020559626839756	13.99448970418928
7	29.665136954243316	31.230800113735057	20.65065327991983	18.4534096521018
8	28.22678068382428	32.475899675772524	19.53363466586715	19.76368497453604
9	27.559490297687578	14.85651269946853	19.041126782077132	38.54287022076676
10	16.313807611269677	26.695588947883646	31.032021785114022	25.958581655732655
11	36.36533929324293	21.92150320176568	22.231952429435502	19.48120507555589
12	25.060054070595207	24.182322868228855	28.243242336687764	22.514380724488177
13	29.433744943460155	19.803936037963975	25.48764421362581	25.274674804950052
14	24.029420431130173	19.94538240316394	25.58713658579127	30.438060579914616
15	25.24980171190869	26.778103627910916	23.51740626275585	24.45468839742455
16	25.31379058197361	26.24431260214352	24.2455376793817	24.19635913650116
17	23.553890239478346	25.991504961459604	25.72393859751878	24.730666201543265
18	24.60815847772542	24.667090162898116	27.18820003952861	23.536551319847852
19	24.86788104469055	25.515819958025364	26.608739537948757	23.00755945933533
20	25.463183952004222	23.751326865985117	26.64935182886899	24.136137353141674
21	26.5809250210415	24.28460185247779	25.381546538743972	23.752926587736738
22	25.23493978079684	24.394208594427706	25.854032098674967	24.51681952610049
23	23.6853254427488	24.323924045211236	26.449438213843578	25.541312298196388
24	23.8912767172884	25.456681857142783	26.223980654719668	24.428060770849147
25	24.509956203746754	24.42568699018545	26.157979231483345	24.906377574584447
26	23.880594704301757	25.198197784436978	26.807930697989562	24.113276813271707
27	24.951634218977137	24.98130647727337	25.958323636095297	24.108735667654194
28	24.026995046539	24.523476432744342	26.802047850257786	24.64748067045887
29	23.760305949365193	25.135602220413787	26.962897292187115	24.1411945380339
30	24.129016011150576	24.973307868515256	26.93601164597435	23.961664474359814
31	24.201932360668106	25.022641223178216	25.79386191924295	24.981564496910728
32	23.896591921817986	25.162333054844137	25.952027956943745	24.98904706639413
33	23.71855837204058	24.695988362282275	26.347520457086947	25.237932808590198
34	24.274126255201033	24.984918752196393	26.677785592905906	24.063169399696672
35	25.264147603745823	24.773187837779957	26.214175908500042	23.748488649974174
36	24.0031024281196	25.49739735591797	26.010701622479083	24.488798593483352
37	25.08879745819695	25.232617604060607	25.82064435760077	23.857940580141673
38	24.101046682460908	25.01443619871021	26.100440852352392	24.78407626647649
39	24.394363406210122	25.036987115015354	25.90145610802147	24.66719337075306
40	24.619098510349428	25.314513036958218	26.09280347108658	23.973584981605782
41	23.436130076987897	25.60942948245905	26.690170535499114	24.264269905053933
42	25.1946887173689	25.596218877026296	25.96446450346443	23.244627902140376
43	24.27985429115039	24.50288646568313	26.121340442978436	25.095918800188045
44	23.90371326380908	25.139678930684056	26.167680769848033	24.78892703565883
45	24.137685470965824	25.29923827442659	25.959097695007372	24.603978559600215
46	23.701037498032598	25.156372962610334	26.286758160524503	24.855831378832562
47	23.964166195780344	25.076644812380827	26.4916775680076	24.467511423831233
48	24.570229591033716	26.08707543513722	25.181013676588897	24.16168129724017
49	23.621646196248705	25.67197344255477	26.043934551770864	24.662445809425666
50	23.547606711816684	26.43608643868728	25.222722665901554	24.793584183594483
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	85.0
1	90.0
2	95.0
3	280.0
4	465.0
5	513.5
6	562.0
7	545.5
8	529.0
9	756.0
10	983.0
11	1480.0
12	1977.0
13	3563.0
14	5149.0
15	7138.5
16	9128.0
17	10225.0
18	11322.0
19	11790.0
20	12258.0
21	12925.0
22	13592.0
23	14402.5
24	15213.0
25	15256.5
26	15300.0
27	17929.5
28	20559.0
29	23786.0
30	27013.0
31	30459.5
32	33906.0
33	38633.5
34	43361.0
35	49033.5
36	54706.0
37	63080.5
38	71455.0
39	77900.5
40	84346.0
41	92531.0
42	100716.0
43	104876.0
44	109036.0
45	121127.5
46	133219.0
47	142665.0
48	152111.0
49	152669.5
50	153228.0
51	143574.5
52	133921.0
53	129461.0
54	125001.0
55	126098.0
56	127195.0
57	122701.0
58	118207.0
59	107991.5
60	97776.0
61	85774.5
62	73773.0
63	64589.5
64	55406.0
65	47625.0
66	39844.0
67	34817.5
68	29791.0
69	27462.0
70	25133.0
71	19677.0
72	14221.0
73	12728.5
74	11236.0
75	8084.5
76	4933.0
77	4538.0
78	4143.0
79	3531.0
80	2919.0
81	2303.5
82	1688.0
83	1401.0
84	1114.0
85	914.5
86	715.0
87	525.0
88	335.0
89	212.5
90	90.0
91	60.0
92	30.0
93	26.0
94	22.0
95	16.5
96	11.0
97	12.5
98	14.0
99	9.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01692608821072154
2	0.003767086705434977
3	5.676432021888322E-4
4	2.0641570988684808E-4
5	0.0
6	5.160392747171202E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.0320785494342404E-4
47	1.0320785494342404E-4
48	0.0
49	0.0
50	5.160392747171202E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1937837.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.07707892351748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.86179132157864	39.50660493933264
2	14.174720907729393	14.763561188613139
3	4.615300436985907	7.210540953379795
4	1.9076126465290097	3.973715773951652
5	0.9713533854441606	2.5292623458200723
6	0.5561443605247177	1.7377424253548928
7	0.36394188876206957	1.326712132524542
8	0.2607015202594285	1.0861258916824998
9	0.186038902567276	0.871952635065655
>10	0.9325450713143995	8.64932074381057
>50	0.07582178067146408	2.7399118186984643
>100	0.08046829863737226	9.186584956609053
>500	0.009671098989914367	3.4926315247850295
>1k	0.00388838000625423	2.925332670372
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2519	0.12999029330124257	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2493	0.12864859118697805	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2153	0.11110325584659597	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2132	0.11001957336969001	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.1603927471712014E-5	0.0	0.0	0.0	5.1603927471712014E-5
2	5.1603927471712014E-5	0.0	0.0	0.0	5.1603927471712014E-5
3	5.1603927471712014E-5	0.0	0.0	0.0	5.1603927471712014E-5
4	5.1603927471712014E-5	0.0	0.0	0.0	5.1603927471712014E-5
5	5.1603927471712014E-5	0.0	0.0	0.0	5.1603927471712014E-5
6	5.1603927471712014E-5	0.0	0.0	0.0	5.1603927471712014E-5
7	5.1603927471712014E-5	0.0	0.0	0.0	5.1603927471712014E-5
8	5.1603927471712014E-5	0.0	0.0	0.0	5.1603927471712014E-5
9	5.1603927471712014E-5	0.0	0.0	0.0	5.1603927471712014E-5
10	5.1603927471712014E-5	0.0	0.0	0.0	5.1603927471712014E-5
11	5.1603927471712014E-5	0.0	0.0	0.0	5.1603927471712014E-5
12	5.1603927471712014E-5	0.0	0.0	1.0320785494342403E-4	5.1603927471712014E-5
13	5.1603927471712014E-5	0.0	0.0	1.0320785494342403E-4	1.0320785494342403E-4
14	5.1603927471712014E-5	0.0	0.0	1.0320785494342403E-4	1.0320785494342403E-4
15	5.1603927471712014E-5	5.1603927471712014E-5	0.0	2.0641570988684806E-4	2.580196373585601E-4
16	5.1603927471712014E-5	5.1603927471712014E-5	0.0	3.612274923019841E-4	3.096235648302721E-4
17	5.1603927471712014E-5	5.1603927471712014E-5	0.0	4.128314197736961E-4	3.096235648302721E-4
18	5.1603927471712014E-5	5.1603927471712014E-5	0.0	5.676432021888321E-4	3.096235648302721E-4
19	5.1603927471712014E-5	5.1603927471712014E-5	0.0	8.772667670191043E-4	3.096235648302721E-4
20	5.1603927471712014E-5	5.1603927471712014E-5	0.0	0.0011868903318493764	3.096235648302721E-4
21	5.1603927471712014E-5	5.1603927471712014E-5	0.0	0.0013933060417362245	3.096235648302721E-4
22	5.1603927471712014E-5	5.1603927471712014E-5	0.0	0.002683404228529025	3.096235648302721E-4
23	5.1603927471712014E-5	5.1603927471712014E-5	0.0	0.005573224166944898	3.096235648302721E-4
24	5.1603927471712014E-5	5.1603927471712014E-5	0.0	0.009701538364681858	3.096235648302721E-4
25	5.1603927471712014E-5	5.1603927471712014E-5	0.0	0.011714091536078627	3.096235648302721E-4
26	5.1603927471712014E-5	5.1603927471712014E-5	0.0	0.015377970386570182	3.096235648302721E-4
27	5.1603927471712014E-5	5.1603927471712014E-5	0.0	0.019093453164533446	3.096235648302721E-4
28	5.1603927471712014E-5	5.1603927471712014E-5	0.0	0.02864017974680017	3.612274923019841E-4
29	5.1603927471712014E-5	5.1603927471712014E-5	0.0	0.042882863728992686	3.612274923019841E-4
30	5.1603927471712014E-5	5.1603927471712014E-5	0.0	0.0742580516317936	3.612274923019841E-4
31	5.1603927471712014E-5	5.1603927471712014E-5	0.0	0.14794846006139836	5.676432021888321E-4
32	5.1603927471712014E-5	5.1603927471712014E-5	0.0	0.22695407302058945	5.676432021888321E-4
33	5.1603927471712014E-5	5.1603927471712014E-5	0.0	0.2963613554700421	5.676432021888321E-4
34	5.1603927471712014E-5	5.1603927471712014E-5	0.0	0.3744897016622141	5.676432021888321E-4
35	5.1603927471712014E-5	1.0320785494342403E-4	0.0	0.4654158218673707	5.676432021888321E-4
36	5.1603927471712014E-5	1.0320785494342403E-4	0.0	0.5939612051994053	5.676432021888321E-4
37	5.1603927471712014E-5	1.0320785494342403E-4	0.0	0.7783420380558324	5.676432021888321E-4
38	5.1603927471712014E-5	1.0320785494342403E-4	0.0	1.0100436724038193	6.192471296605442E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	545	0.0	18.572031	1
TAGGACG	145	6.3118932E-9	18.207237	4
ATACCGT	605	0.0	18.18216	6
AATACCG	85	1.4311905E-4	18.117987	5
CTAGCGG	600	0.0	17.599876	29
GTCGTAG	530	0.0	17.43384	11
GGTTGCG	90	2.2163584E-4	17.11099	7
CGCAATA	620	0.0	17.032139	36
CGTCGTA	545	0.0	16.95401	10
ACCGTCG	560	0.0	16.892736	8
CCGTCGT	565	0.0	16.743244	9
CGGTCCA	660	0.0	16.66655	10
CGAATGC	665	0.0	16.541237	43
TACCGTC	575	0.0	16.452059	7
TCTAGCG	645	0.0	16.371979	28
GTGTACG	110	5.615706E-5	16.002777	1
TAGCGGC	660	0.0	15.9998865	30
TATTAGC	455	0.0	15.956343	2
CAATACG	680	0.0	15.85283	38
CGCCGGT	700	0.0	15.714176	7
>>END_MODULE