FastQCFastQC Report
Thu 2 Feb 2017
SRR4062700_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062700_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664996
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG20880.3139868510487281No Hit
TCCATGTACTCTGCGTTGATACCAC17310.2603023176079255No Hit
GTATCAACGCAGAGTACTTTTTTTT15550.23383599299845412No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10420.15669267183562008No Hit
TATCAACGCAGAGTACTTTTTTTTT10060.1512791054382282No Hit
GAGTACATGGAAGCAGTGGTATCAA9620.14466252428586038No Hit
CATGTACTCTGCGTTGATACCACTG8780.13203086935861266No Hit
TTGTAGAACAGTGTATATCAATGAG7790.11714356176578507No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7490.11263225643462517No Hit
GTGTATATCAATGAGTTACAATGAA7120.1070683131928613No Hit
GATATACACTGTTCTACAAATCCCG7070.10631642897100133No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTG350.002168383716.285786
AGAACCG350.002168383716.285785
TACTCCG456.7549216E-414.7778365
AGGCCCG1058.731149E-1114.47733710
CGTCACT400.005274756314.25005615
GATATAT400.00528530214.2457681
ATTACCG903.6261554E-813.72227715
AGACGGA1257.2759576E-1213.6800546
TATACCT852.693505E-713.4108094
TAATACC500.001498776813.2990524
ACAGTCC655.4392844E-513.1548878
TTTCGGA655.4392844E-513.15488717
GTTTTCG655.443128E-513.15389715
TTACCGC957.361268E-813.000051516
AGCGAAA1051.983426E-812.6676717
AAGACGG1207.366907E-1012.6667175
CGAGCCG1207.366907E-1012.66671715
TCTAGAC604.094204E-412.6638573
TCCAACG1457.2759576E-1212.45019918
CGGACCA1155.293259E-912.3922869