##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062700_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	664996
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.144393349734436	32.0	32.0	32.0	32.0	32.0
2	30.771890357235232	32.0	32.0	32.0	32.0	32.0
3	30.756291767168523	32.0	32.0	32.0	32.0	32.0
4	30.84343665225054	32.0	32.0	32.0	32.0	32.0
5	30.567147772317426	32.0	32.0	32.0	32.0	32.0
6	34.21911710747132	36.0	36.0	36.0	32.0	36.0
7	34.11166232578842	36.0	36.0	36.0	32.0	36.0
8	34.10141865514981	36.0	36.0	36.0	32.0	36.0
9	34.30590559943218	36.0	36.0	36.0	32.0	36.0
10	33.91133480502138	36.0	36.0	36.0	32.0	36.0
11	34.282579143333194	36.0	36.0	36.0	32.0	36.0
12	34.04372507503805	36.0	36.0	36.0	32.0	36.0
13	34.1702611745033	36.0	36.0	36.0	32.0	36.0
14	34.06543498006003	36.0	36.0	36.0	32.0	36.0
15	33.985375851884825	36.0	36.0	36.0	32.0	36.0
16	33.9823517735445	36.0	36.0	36.0	32.0	36.0
17	33.904286642325665	36.0	36.0	36.0	32.0	36.0
18	33.91835740365356	36.0	36.0	36.0	32.0	36.0
19	33.90376333090725	36.0	36.0	36.0	32.0	36.0
20	33.91423858188621	36.0	36.0	36.0	32.0	36.0
21	33.86841574986917	36.0	36.0	36.0	32.0	36.0
22	33.82198990670621	36.0	36.0	36.0	32.0	36.0
23	33.7480255520334	36.0	36.0	36.0	32.0	36.0
24	33.72993972896077	36.0	36.0	36.0	32.0	36.0
25	33.22365247309758	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	14.0
5	60.0
6	98.0
7	23.0
8	108.0
9	133.0
10	88.0
11	24.0
12	56.0
13	28.0
14	84.0
15	109.0
16	173.0
17	255.0
18	345.0
19	516.0
20	785.0
21	1169.0
22	1833.0
23	2732.0
24	3992.0
25	5651.0
26	8118.0
27	10915.0
28	14767.0
29	20221.0
30	26832.0
31	36753.0
32	52594.0
33	73903.0
34	153996.0
35	248616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.31257434780712	17.082964257409213	11.04235061636306	25.5621107784206
2	16.054341565673504	19.7897978733163	38.48121519626936	25.67464536474084
3	20.540833988748417	23.15582737945751	28.081440900607994	28.22189773118608
4	12.369644011971545	14.855995548269691	36.07240077604488	36.701959663713886
5	13.98095960120157	36.93037122610361	34.284300320248136	14.804368852446686
6	34.479900227016635	35.15056964777068	16.82480604294511	13.544724082267578
7	29.879848023172705	30.345709664071023	20.441485159688522	19.33295715306775
8	27.493385188239934	34.338172656369935	19.604526066422043	18.56391608896809
9	26.710326682481007	14.369573855768419	18.514666393728852	40.40543306802172
10	14.968014296833951	27.68701737270082	32.90409709079905	24.440871239666187
11	37.719075055742664	21.045507992165163	21.90863473012422	19.326782221967953
12	23.4506258629802	23.762616262348992	30.166701047848065	22.620056826822744
13	29.88175212813047	19.66296194809379	25.428863079985188	25.02642284379056
14	22.548526586486634	19.88626440139	25.797383810217834	31.76782520190553
15	24.81267868353424	27.897138504109254	23.069766181445367	24.22041663091114
16	24.876549900938823	26.228328485658714	25.191046555742236	23.70407505766023
17	23.217307773381275	26.268361428803725	26.456116260441696	24.0582145373733
18	23.976974491555154	25.656005649470814	27.643408775049576	22.723611083924457
19	24.709010802362243	25.77915839750404	25.566996027790413	23.944834772343302
20	24.437144121818363	25.246687001845824	26.69353600260162	23.622632873734194
21	25.388331094795554	25.072727382235847	25.01520800332468	24.523733519643923
22	24.171708181033665	25.673243602660893	25.963158917267958	24.19188929903748
23	23.726820129930758	25.234848188227925	25.831041825415706	25.207289856425607
24	23.71723925040845	26.00170156378229	26.081961165194738	24.19909802061452
25	24.484384340522414	25.27476664222289	25.909301033414696	24.33154798384
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	74.0
1	74.0
2	213.5
3	353.0
4	353.0
5	353.0
6	979.0
7	1605.0
8	1605.0
9	1605.0
10	1697.5
11	1790.0
12	1790.0
13	1790.0
14	1895.0
15	2000.0
16	2000.0
17	2000.0
18	3323.5
19	4647.0
20	4647.0
21	4647.0
22	7549.5
23	10452.0
24	10452.0
25	10452.0
26	15943.5
27	21435.0
28	21435.0
29	21435.0
30	28388.5
31	35342.0
32	35342.0
33	35342.0
34	40319.5
35	45297.0
36	45297.0
37	45297.0
38	50527.0
39	55757.0
40	55757.0
41	55757.0
42	63334.0
43	70911.0
44	70911.0
45	70911.0
46	81641.0
47	92371.0
48	92371.0
49	92371.0
50	90083.5
51	87796.0
52	87796.0
53	87796.0
54	80728.0
55	73660.0
56	73660.0
57	73660.0
58	66564.5
59	59469.0
60	59469.0
61	59469.0
62	51876.0
63	44283.0
64	44283.0
65	44283.0
66	36309.0
67	28335.0
68	28335.0
69	28335.0
70	21282.0
71	14229.0
72	14229.0
73	14229.0
74	10944.0
75	7659.0
76	7659.0
77	7659.0
78	5993.5
79	4328.0
80	4328.0
81	4328.0
82	3041.0
83	1754.0
84	1754.0
85	1754.0
86	1286.0
87	818.0
88	818.0
89	818.0
90	549.0
91	280.0
92	280.0
93	280.0
94	172.5
95	65.0
96	65.0
97	65.0
98	175.5
99	286.0
100	286.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0070677116854838224
2	0.0025564063543239354
3	0.003458667420555913
4	0.012932408615991677
5	0.02992499203002725
6	0.043158154334762916
7	0.06330865148061042
8	0.08691781604701382
9	0.09323364351063766
10	0.119248837586993
11	0.11774506914327303
12	0.1312789851367527
13	0.12000072180885298
14	0.1239105197625249
15	0.11473753225583311
16	0.11428640172271712
17	0.12511353451750085
18	0.12977521669303274
19	0.13263237673610068
20	0.119399214431365
21	0.1311286082923807
22	0.1517302359713442
23	0.14285800215339642
24	0.1354895367791686
25	0.13338426095796063
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	664996.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.606345464586475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.56255219123248	50.24770767266433
2	11.82573988443268	14.570812333893974
3	3.1201588104919926	5.766647447506287
4	1.2129934171681445	2.9891236601731985
5	0.5837460669278888	1.798123093137657
6	0.36221131723506184	1.3388709324459687
7	0.23410690250095142	1.0095729497782515
8	0.16434125326913962	0.8099571218385374
9	0.11933494215401513	0.661661070510303
>10	0.6698033949409457	7.826326117545062
>50	0.08143168089748558	3.4930130749643657
>100	0.05893389784074671	6.946542425075992
>500	0.003423545932605968	1.4235063841683517
>1k	0.001222694975930703	1.118135716297757
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	2088	0.3139868510487281	No Hit
TCCATGTACTCTGCGTTGATACCAC	1731	0.2603023176079255	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1555	0.23383599299845412	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1042	0.15669267183562008	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1006	0.1512791054382282	No Hit
GAGTACATGGAAGCAGTGGTATCAA	962	0.14466252428586038	No Hit
CATGTACTCTGCGTTGATACCACTG	878	0.13203086935861266	No Hit
TTGTAGAACAGTGTATATCAATGAG	779	0.11714356176578507	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	749	0.11263225643462517	No Hit
GTGTATATCAATGAGTTACAATGAA	712	0.1070683131928613	No Hit
GATATACACTGTTCTACAAATCCCG	707	0.10631642897100133	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5037684437199623E-4
2	0.0	0.0	0.0	0.0	1.5037684437199623E-4
3	0.0	0.0	0.0	0.0	1.5037684437199623E-4
4	0.0	0.0	0.0	0.0	1.5037684437199623E-4
5	0.0	0.0	0.0	0.0	1.5037684437199623E-4
6	0.0	0.0	0.0	0.0	1.5037684437199623E-4
7	0.0	0.0	0.0	0.0	1.5037684437199623E-4
8	0.0	0.0	0.0	0.0	1.5037684437199623E-4
9	0.0	0.0	0.0	0.0	1.5037684437199623E-4
10	0.0	0.0	0.0	0.0	1.5037684437199623E-4
11	0.0	0.0	0.0	0.0	1.5037684437199623E-4
12	0.0	0.0	0.0	0.0	7.518842218599811E-4
13	0.0	0.0	0.0	0.0	0.0010526379106039737
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCTG	35	0.0021683837	16.28578	6
AGAACCG	35	0.0021683837	16.28578	5
TACTCCG	45	6.7549216E-4	14.777836	5
AGGCCCG	105	8.731149E-11	14.477337	10
CGTCACT	40	0.0052747563	14.250056	15
GATATAT	40	0.005285302	14.245768	1
ATTACCG	90	3.6261554E-8	13.722277	15
AGACGGA	125	7.2759576E-12	13.680054	6
TATACCT	85	2.693505E-7	13.410809	4
TAATACC	50	0.0014987768	13.299052	4
ACAGTCC	65	5.4392844E-5	13.154887	8
TTTCGGA	65	5.4392844E-5	13.154887	17
GTTTTCG	65	5.443128E-5	13.153897	15
TTACCGC	95	7.361268E-8	13.0000515	16
AGCGAAA	105	1.983426E-8	12.66767	17
AAGACGG	120	7.366907E-10	12.666717	5
CGAGCCG	120	7.366907E-10	12.666717	15
TCTAGAC	60	4.094204E-4	12.663857	3
TCCAACG	145	7.2759576E-12	12.450199	18
CGGACCA	115	5.293259E-9	12.392286	9
>>END_MODULE