FastQCFastQC Report
Thu 2 Feb 2017
SRR4062700_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062700_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664996
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA7110.10691793634848931No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG353.2164465E-431.4283565
ATACGAA1700.028.47039440
TTATACG407.0290646E-427.49981139
CGCAATA1850.026.16198336
CAATACG1850.026.16198338
AATACGA1850.026.16198339
CGAATGC1800.025.6664943
TACGAAT1900.025.4735141
TAGCGGC2050.024.68275630
CTAGCGG2050.023.60959229
CGGTCCA2150.023.53472310
TCTAGCG2150.022.51147528
CCGGTCC2250.021.5109639
CAACGAA2600.021.15370230
GCGAAAG1700.020.7057418
TCTTGCG2350.020.59872
TGTAGGA3000.020.5362822
GTCCTAC2800.020.4315031
GGTCTAC1301.6170816E-920.3106081
CGAAAGC1750.020.1141519
Produced by FastQC (version 0.11.5)