##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062700_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	664996
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13870760124873	32.0	32.0	32.0	32.0	32.0
2	31.192437247742845	32.0	32.0	32.0	32.0	32.0
3	31.38305643943723	32.0	32.0	32.0	32.0	32.0
4	31.533585164422043	32.0	32.0	32.0	32.0	32.0
5	31.423172470210346	32.0	32.0	32.0	32.0	32.0
6	35.037968649435484	36.0	36.0	36.0	36.0	36.0
7	35.04649200897449	36.0	36.0	36.0	36.0	36.0
8	34.96504640629417	36.0	36.0	36.0	36.0	36.0
9	35.13387599323906	36.0	36.0	36.0	36.0	36.0
10	34.97967807325157	36.0	36.0	36.0	36.0	36.0
11	35.15143700112482	36.0	36.0	36.0	36.0	36.0
12	35.04331605002135	36.0	36.0	36.0	36.0	36.0
13	35.09321710205776	36.0	36.0	36.0	36.0	36.0
14	35.05415671673213	36.0	36.0	36.0	36.0	36.0
15	35.00451130533116	36.0	36.0	36.0	36.0	36.0
16	35.027270840726864	36.0	36.0	36.0	36.0	36.0
17	34.989814976330685	36.0	36.0	36.0	36.0	36.0
18	35.013103838218576	36.0	36.0	36.0	36.0	36.0
19	34.99457891476039	36.0	36.0	36.0	36.0	36.0
20	34.98532321998929	36.0	36.0	36.0	36.0	36.0
21	34.95752455653869	36.0	36.0	36.0	32.0	36.0
22	34.942789129558676	36.0	36.0	36.0	32.0	36.0
23	34.89227453999723	36.0	36.0	36.0	32.0	36.0
24	34.8685886832402	36.0	36.0	36.0	32.0	36.0
25	34.840221595317864	36.0	36.0	36.0	32.0	36.0
26	34.778830248603	36.0	36.0	36.0	32.0	36.0
27	34.77509037648347	36.0	36.0	36.0	32.0	36.0
28	34.7391277541519	36.0	36.0	36.0	32.0	36.0
29	34.71829153859572	36.0	36.0	36.0	32.0	36.0
30	34.69276657303202	36.0	36.0	36.0	32.0	36.0
31	34.68567931235677	36.0	36.0	36.0	32.0	36.0
32	34.64345199068867	36.0	36.0	36.0	32.0	36.0
33	34.615093925376996	36.0	36.0	36.0	32.0	36.0
34	34.59806976282564	36.0	36.0	36.0	32.0	36.0
35	34.5661116758597	36.0	36.0	36.0	32.0	36.0
36	34.52623775180603	36.0	36.0	36.0	32.0	36.0
37	34.52007982002899	36.0	36.0	36.0	32.0	36.0
38	34.46025540003248	36.0	36.0	36.0	32.0	36.0
39	34.41639047452917	36.0	36.0	36.0	32.0	36.0
40	34.383227869039814	36.0	36.0	36.0	32.0	36.0
41	34.36372248855632	36.0	36.0	36.0	32.0	36.0
42	34.30555371761635	36.0	36.0	36.0	32.0	36.0
43	34.31176428128891	36.0	36.0	36.0	32.0	36.0
44	34.27688737977371	36.0	36.0	36.0	32.0	36.0
45	34.18914850615643	36.0	36.0	36.0	32.0	36.0
46	34.21794867939055	36.0	36.0	36.0	32.0	36.0
47	34.174808570277115	36.0	36.0	36.0	32.0	36.0
48	34.14785652846032	36.0	36.0	36.0	32.0	36.0
49	34.15013924895789	36.0	36.0	36.0	32.0	36.0
50	33.58598247207502	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	15.0
22	46.0
23	152.0
24	459.0
25	1102.0
26	2281.0
27	4403.0
28	7568.0
29	11590.0
30	17118.0
31	24846.0
32	37895.0
33	61401.0
34	130218.0
35	365898.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.64186613125183	17.862852587963694	11.588873598483994	25.906407682300483
2	15.60831681619812	20.19952899847511	38.02765855697886	26.164495628347918
3	19.96153348752019	23.50397146440419	28.352135507989544	28.182359540086075
4	12.393777396822532	15.539816088842773	36.06839149166535	35.99801502266934
5	13.971362233757798	37.24022400134738	33.98967813340231	14.798735631492521
6	34.938262508026405	35.30337913331418	16.398969613213975	13.359388745445441
7	29.993458597433065	30.41466477191558	20.285866811028654	19.306009819622705
8	27.618403145888315	34.67770434364168	19.276987045015375	18.426905465454627
9	26.867078899722706	14.213468953196712	18.229733712683984	40.689718434396596
10	15.050165715282498	27.828588442637248	32.64801592791536	24.4732299141649
11	37.938574066610926	21.00042707023802	21.680581537332554	19.380417325818502
12	23.58540502499263	23.737887145185834	29.99431575528274	22.682392074538793
13	30.12935416152879	19.46553061973305	25.34631787258871	25.058797346149454
14	22.67532436285331	19.771848251718808	25.51504069197409	32.037786693453796
15	25.061804883036892	27.952799716088517	22.652467082508768	24.332928318365823
16	25.091278744533803	26.223616382654935	24.92917250630079	23.755932366510475
17	23.349914886706085	26.482414931819136	26.106021690356034	24.061648491118742
18	24.229920179971007	25.755944396658027	27.15610319460568	22.858032228765286
19	25.037594211093	25.867072884648927	25.19564027452797	23.899692629730104
20	24.774585110286377	25.422107802152194	26.10677357457789	23.696533512983535
21	25.694289890465505	25.245565386859468	24.641952733550276	24.418191989124747
22	24.41909425019098	25.716395286588188	25.57173276230233	24.292777700918503
23	23.98916685213144	25.201354594614102	25.56496580430559	25.244512748948868
24	24.04781382143652	26.32226359256296	25.432483804413863	24.197438781586655
25	24.72706602746483	25.414438583089222	25.466468971241934	24.39202641820402
26	23.2741249571426	26.20060872546602	26.29835367430782	24.22691264308357
27	24.436838717826877	25.72391412880679	26.03895361776612	23.800293535600215
28	23.817737249547367	25.910832546361178	25.684515395581325	24.586914808510127
29	23.401794898014426	25.72692166569423	26.000156391918143	24.871127044373196
30	23.954580177925884	26.206623799240898	25.944968090033626	23.893827932799596
31	24.050219850946473	25.749929322883148	25.425566469572747	24.774284356597633
32	23.747511263225643	26.179706344098307	25.63594367484917	24.436838717826877
33	23.837887746693212	25.388122635324123	26.022712918573948	24.751276699408717
34	23.924805562740225	26.354143483569825	26.172939386101568	23.548111567588375
35	24.57668918309283	25.909328777917462	25.953990700695943	23.559991338293766
36	23.419840119339064	26.580460634349677	25.795493506727862	24.204205739583394
37	24.38525946020728	26.06587107290871	25.69353800624365	23.855331460640365
38	24.122701489933775	25.970532153576865	25.39714524598644	24.50962111050292
39	24.378342125366167	25.470378769195605	25.651883620352606	24.499395485085625
40	24.997293216801303	25.884666975440453	25.494138310606377	23.623901497151863
41	23.462246389451966	25.620755613567603	27.0126436850748	23.904354311905635
42	25.06616581152368	26.22406751318805	25.760455701989184	22.949310973299085
43	23.984204416267165	25.29970105083339	26.142412886694053	24.573681646205394
44	23.416381451918507	26.058051477001364	26.19775156542295	24.327815505657178
45	23.723450968126127	26.179706344098307	25.9960962171201	24.100746470655462
46	23.4980360784125	25.415340844155455	26.0562469548689	25.03037612256314
47	24.084355394618914	25.537597218629887	26.49384357199141	23.88420381475979
48	24.198641796341633	26.239255574469624	25.1627077456105	24.399394883578246
49	23.677435653748294	25.94827638060981	26.366023254275213	24.008264711366685
50	23.467208825316245	26.632791776191134	25.150978351749487	24.749021046743138
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	5.5
2	4.0
3	25.5
4	47.0
5	71.0
6	95.0
7	76.0
8	57.0
9	84.5
10	112.0
11	173.5
12	235.0
13	421.5
14	608.0
15	853.5
16	1099.0
17	1316.0
18	1533.0
19	1763.0
20	1993.0
21	2574.5
22	3156.0
23	3631.0
24	4106.0
25	4883.5
26	5661.0
27	7551.0
28	9441.0
29	10762.5
30	12084.0
31	13317.5
32	14551.0
33	16717.0
34	18883.0
35	21575.0
36	24267.0
37	26586.0
38	28905.0
39	30256.5
40	31608.0
41	33188.0
42	34768.0
43	35966.5
44	37165.0
45	40599.5
46	44034.0
47	47156.5
48	50279.0
49	50843.0
50	51407.0
51	48952.5
52	46498.0
53	44849.0
54	43200.0
55	43034.5
56	42869.0
57	40915.5
58	38962.0
59	35715.5
60	32469.0
61	28673.5
62	24878.0
63	21564.5
64	18251.0
65	15653.0
66	13055.0
67	11348.5
68	9642.0
69	8700.5
70	7759.0
71	5869.0
72	3979.0
73	3542.0
74	3105.0
75	2266.0
76	1427.0
77	1275.5
78	1124.0
79	929.5
80	735.0
81	555.0
82	375.0
83	308.0
84	241.0
85	217.5
86	194.0
87	139.0
88	84.0
89	55.0
90	26.0
91	17.0
92	8.0
93	6.5
94	5.0
95	4.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013684292837851657
2	0.004511305331159887
3	3.0075368874399246E-4
4	1.5037684437199623E-4
5	0.0
6	4.511305331159887E-4
7	1.5037684437199623E-4
8	1.5037684437199623E-4
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	664996.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.05761946628927
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.02245290497213	54.1820798168828
2	11.805108851065055	15.596347764835572
3	3.0406438640387634	6.0257308590953995
4	1.1022486542614154	2.9124768864172
5	0.56469216060497	1.86511099304199
6	0.3153634605611569	1.2499295682792613
7	0.20821336586480055	0.9627855503064578
8	0.14869793571243037	0.7858105322171575
9	0.1041343938350366	0.6190983145176792
>10	0.5826046647013716	7.309642328127451
>50	0.06172091266019198	2.8662243417623348
>100	0.04297585680773016	5.151968255615245
>500	0.0011429749150276192	0.4727947889015117
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	711	0.10691793634848931	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.5037684437199623E-4
7	0.0	0.0	0.0	0.0	1.5037684437199623E-4
8	0.0	0.0	0.0	0.0	1.5037684437199623E-4
9	0.0	0.0	0.0	0.0	1.5037684437199623E-4
10	0.0	0.0	0.0	0.0	1.5037684437199623E-4
11	0.0	0.0	0.0	0.0	1.5037684437199623E-4
12	0.0	0.0	0.0	0.0	9.022610662319774E-4
13	0.0	0.0	0.0	0.0	9.022610662319774E-4
14	0.0	0.0	0.0	1.5037684437199623E-4	9.022610662319774E-4
15	0.0	0.0	0.0	3.0075368874399246E-4	9.022610662319774E-4
16	0.0	0.0	0.0	3.0075368874399246E-4	0.0010526379106039737
17	0.0	0.0	0.0	4.511305331159887E-4	0.0010526379106039737
18	0.0	0.0	0.0	7.518842218599811E-4	0.0010526379106039737
19	0.0	0.0	0.0	7.518842218599811E-4	0.0010526379106039737
20	0.0	0.0	0.0	0.001353391599347966	0.001353391599347966
21	0.0	0.0	0.0	0.0016541452880919584	0.0015037684437199622
22	0.0	0.0	0.0	0.002706783198695932	0.0015037684437199622
23	0.0	0.0	0.0	0.004060174798043898	0.0015037684437199622
24	0.0	0.0	0.0	0.007518842218599811	0.0016541452880919584
25	0.0	0.0	0.0	0.010225625417295743	0.0016541452880919584
26	0.0	0.0	0.0	0.01473693074845563	0.0018045221324639548
27	0.0	0.0	0.0	0.01789484448026755	0.0018045221324639548
28	0.0	0.0	0.0	0.024511425632635385	0.0018045221324639548
29	0.0	0.0	0.0	0.0357896889605351	0.0018045221324639548
30	0.0	0.0	0.0	0.057594331394474556	0.0018045221324639548
31	0.0	0.0	0.0	0.09293288982189367	0.0019548989768359507
32	0.0	0.0	0.0	0.13819631997786452	0.0019548989768359507
33	0.0	0.0	0.0	0.18436201120006737	0.0019548989768359507
34	0.0	0.0	0.0	0.24045257415082197	0.0021052758212079473
35	0.0	0.0	0.0	0.3019567034989684	0.0021052758212079473
36	0.0	0.0	0.0	0.39639336176458206	0.0021052758212079473
37	0.0	0.0	0.0	0.5209053889045949	0.0021052758212079473
38	0.0	0.0	0.0	0.6729363785646831	0.0021052758212079473
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGCCG	35	3.2164465E-4	31.428356	5
ATACGAA	170	0.0	28.470394	40
TTATACG	40	7.0290646E-4	27.499811	39
CGCAATA	185	0.0	26.161983	36
CAATACG	185	0.0	26.161983	38
AATACGA	185	0.0	26.161983	39
CGAATGC	180	0.0	25.66649	43
TACGAAT	190	0.0	25.47351	41
TAGCGGC	205	0.0	24.682756	30
CTAGCGG	205	0.0	23.609592	29
CGGTCCA	215	0.0	23.534723	10
TCTAGCG	215	0.0	22.511475	28
CCGGTCC	225	0.0	21.510963	9
CAACGAA	260	0.0	21.153702	30
GCGAAAG	170	0.0	20.70574	18
TCTTGCG	235	0.0	20.5987	2
TGTAGGA	300	0.0	20.536282	2
GTCCTAC	280	0.0	20.431503	1
GGTCTAC	130	1.6170816E-9	20.310608	1
CGAAAGC	175	0.0	20.11415	19
>>END_MODULE