FastQCFastQC Report
Thu 2 Feb 2017
SRR4062699_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062699_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508270
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG31260.20725732130188892No Hit
GTATCAACGCAGAGTACTTTTTTTT29970.19870447598904709No Hit
TCCATGTACTCTGCGTTGATACCAC25880.1715873152684864No Hit
TATCAACGCAGAGTACTTTTTTTTT18840.12491132224336493No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15550.10309825163929535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCAA400.005277903314.25044710
TGAAGCG1001.0084477E-813.2986535
GTATAGG1301.4551915E-1113.1472781
CGCTTAC604.0850884E-412.669167518
AACCGCG1155.320544E-912.3916937
GCACCGT701.0905374E-412.214676
ACAGCGT701.0905374E-412.214678
CGCATCG1501.4551915E-1112.0349113
CGAACGA802.861411E-511.8769516
TTAGAAC802.8740746E-511.8718263
CGTCTTA1052.7148417E-711.76344615
GCCGTTA752.0712122E-411.40149415
AGACCGT752.073129E-411.4003586
ACCGTGG752.073129E-411.4003588
GTCCTAA1001.9408872E-611.3943061
CCGTCCA1104.965168E-711.2276259
AAGCGTG855.32067E-511.1768237
GTATTAT855.3495252E-511.170891
GCGTTAT855.3495252E-511.170891
ACGGTAT2300.011.1525249
Produced by FastQC (version 0.11.5)