##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062699_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1508270
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.208759704827386	32.0	32.0	32.0	32.0	32.0
2	30.977046550020884	32.0	32.0	32.0	32.0	32.0
3	30.955751291214437	32.0	32.0	32.0	32.0	32.0
4	30.99347663216798	32.0	32.0	32.0	32.0	32.0
5	30.868241097416245	32.0	32.0	32.0	32.0	32.0
6	34.455077671769644	36.0	36.0	36.0	32.0	36.0
7	34.37724014931014	36.0	36.0	36.0	32.0	36.0
8	34.38300238021044	36.0	36.0	36.0	32.0	36.0
9	34.485816863028504	36.0	36.0	36.0	32.0	36.0
10	34.265236330365255	36.0	36.0	36.0	32.0	36.0
11	34.47036405948537	36.0	36.0	36.0	32.0	36.0
12	34.31323436785191	36.0	36.0	36.0	32.0	36.0
13	34.400705443985494	36.0	36.0	36.0	32.0	36.0
14	34.33156861835083	36.0	36.0	36.0	32.0	36.0
15	34.27952157107149	36.0	36.0	36.0	32.0	36.0
16	34.2743447791178	36.0	36.0	36.0	32.0	36.0
17	34.21813335808575	36.0	36.0	36.0	32.0	36.0
18	34.22054141499864	36.0	36.0	36.0	32.0	36.0
19	34.20990141022496	36.0	36.0	36.0	32.0	36.0
20	34.18670662414554	36.0	36.0	36.0	32.0	36.0
21	34.16874763802237	36.0	36.0	36.0	32.0	36.0
22	34.12257354452452	36.0	36.0	36.0	32.0	36.0
23	34.084530621175254	36.0	36.0	36.0	32.0	36.0
24	34.06326387185318	36.0	36.0	36.0	32.0	36.0
25	33.56533445603241	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	56.0
5	125.0
6	258.0
7	50.0
8	227.0
9	261.0
10	179.0
11	60.0
12	107.0
13	67.0
14	248.0
15	362.0
16	643.0
17	842.0
18	1122.0
19	1520.0
20	2135.0
21	2848.0
22	3986.0
23	5423.0
24	7541.0
25	10486.0
26	14441.0
27	19136.0
28	26156.0
29	36364.0
30	47844.0
31	67853.0
32	100490.0
33	150823.0
34	355463.0
35	651152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.82582415323634	17.035660814479158	10.806389798389288	26.332125233895216
2	17.25226440777308	19.99919110534129	36.13305919318725	26.615485293698377
3	19.410159759449147	23.787482470884264	27.96430193508177	28.838055834584818
4	13.010683811691187	15.256392913884978	35.365923202809405	36.36700007161443
5	14.945329567929155	36.13600820288613	33.45481581310578	15.46384641607893
6	34.3747699153023	34.78694903213525	16.88203147127515	13.956249581287306
7	30.14767396422892	30.217334623935272	20.953130317845	18.681861093990808
8	28.470063311340365	32.06237305051039	19.75730542263853	19.710258215510716
9	27.585964334821593	14.131756236628213	18.350604948954942	39.931674479595245
10	15.915147709600145	26.549030878730573	31.67477669489191	25.86104471677737
11	37.72309219993734	21.11795676485113	21.713223307260552	19.445727727950977
12	24.397829710763087	23.716046079810507	28.57397020466122	23.312154004765183
13	29.749398953096435	19.425198700596997	24.998506509626708	25.82689583667986
14	23.687798544417568	19.256197908230867	24.82940318996121	32.22660035739036
15	25.434480176924897	26.49506049270291	22.42630563085247	25.644153699519723
16	26.14590392320972	25.452178974664967	23.383283034070974	25.018634068054336
17	24.318133089587775	25.31867142740505	25.15889594945599	25.20429953355119
18	25.192265180900208	24.61445727945682	25.813156415068413	24.38012112457456
19	25.568007180208223	25.141350391638667	25.106631004611767	24.18401142354134
20	25.58470963155411	24.129034056701652	25.206818110914114	25.07943820083013
21	26.580280268983874	24.26311561925372	24.174627093553546	24.981977018208852
22	25.686165540237717	24.35211246096673	24.682106677768285	25.279615321027272
23	24.61487392614821	24.145300280164115	25.138023979923783	26.101801813763892
24	24.652862605555264	25.102700621381913	24.95505603059111	25.289380742471717
25	25.076807260908357	24.394437481412247	24.97822577218847	25.55052948549093
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	154.0
1	154.0
2	431.5
3	709.0
4	709.0
5	709.0
6	1802.5
7	2896.0
8	2896.0
9	2896.0
10	3258.0
11	3620.0
12	3620.0
13	3620.0
14	4141.0
15	4662.0
16	4662.0
17	4662.0
18	7318.5
19	9975.0
20	9975.0
21	9975.0
22	15452.5
23	20930.0
24	20930.0
25	20930.0
26	30283.0
27	39636.0
28	39636.0
29	39636.0
30	49291.5
31	58947.0
32	58947.0
33	58947.0
34	73715.5
35	88484.0
36	88484.0
37	88484.0
38	102424.0
39	116364.0
40	116364.0
41	116364.0
42	135441.0
43	154518.0
44	154518.0
45	154518.0
46	178038.0
47	201558.0
48	201558.0
49	201558.0
50	202585.0
51	203612.0
52	203612.0
53	203612.0
54	190745.0
55	177878.0
56	177878.0
57	177878.0
58	163212.0
59	148546.0
60	148546.0
61	148546.0
62	131544.0
63	114542.0
64	114542.0
65	114542.0
66	95253.5
67	75965.0
68	75965.0
69	75965.0
70	58268.0
71	40571.0
72	40571.0
73	40571.0
74	31481.5
75	22392.0
76	22392.0
77	22392.0
78	17606.5
79	12821.0
80	12821.0
81	12821.0
82	9115.5
83	5410.0
84	5410.0
85	5410.0
86	3943.5
87	2477.0
88	2477.0
89	2477.0
90	1649.5
91	822.0
92	822.0
93	822.0
94	479.0
95	136.0
96	136.0
97	136.0
98	390.5
99	645.0
100	645.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007690930668912065
2	0.002585743931789401
3	0.004309573219649002
4	0.012862418532490866
5	0.03368097223971835
6	0.044620658104981205
7	0.06384798477726136
8	0.0844013339786643
9	0.09056733873908518
10	0.11264561384897928
11	0.11390533525164592
12	0.12776227068097887
13	0.11483355102203185
14	0.11894422086231246
15	0.1088664496409794
16	0.1074741259854005
17	0.11808230621838266
18	0.12511022562273333
19	0.12643624815185608
20	0.11622587467761078
21	0.1233200952084176
22	0.14473535905375032
23	0.13326526417683837
24	0.12928719658947005
25	0.1261047425195754
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1508270.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.57912924321082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.31065942297259	45.439071790998526
2	12.290303542489045	13.907493451375588
3	3.314412710719124	5.625797553753542
4	1.347432858547648	3.049463114012655
5	0.6740896288357189	1.9069702115702074
6	0.4155900384964043	1.4108233500167413
7	0.2783143972456162	1.1022750376404225
8	0.19839537196320645	0.8980029913248915
9	0.15524001675023502	0.7905010474286785
>10	0.8460479916341727	8.901368534371787
>50	0.08119034691355385	3.2014285525107913
>100	0.07916254535356329	9.215280749959298
>500	0.00669467051937349	2.497613114787766
>1k	0.0024664575597691807	2.0539105002490428
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3126	0.20725732130188892	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2997	0.19870447598904709	No Hit
TCCATGTACTCTGCGTTGATACCAC	2588	0.1715873152684864	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1884	0.12491132224336493	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1555	0.10309825163929535	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.630112645613849E-5
2	0.0	0.0	0.0	0.0	6.630112645613849E-5
3	0.0	0.0	0.0	0.0	6.630112645613849E-5
4	0.0	0.0	0.0	6.630112645613849E-5	6.630112645613849E-5
5	0.0	0.0	0.0	1.3260225291227698E-4	6.630112645613849E-5
6	0.0	0.0	0.0	1.3260225291227698E-4	1.3260225291227698E-4
7	0.0	0.0	0.0	1.3260225291227698E-4	1.3260225291227698E-4
8	0.0	0.0	0.0	1.3260225291227698E-4	1.3260225291227698E-4
9	0.0	0.0	0.0	1.9890337936841548E-4	1.3260225291227698E-4
10	0.0	0.0	0.0	1.9890337936841548E-4	1.3260225291227698E-4
11	0.0	0.0	0.0	1.9890337936841548E-4	1.3260225291227698E-4
12	0.0	0.0	0.0	1.9890337936841548E-4	1.9890337936841548E-4
13	0.0	0.0	0.0	2.6520450582455396E-4	2.6520450582455396E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCCAA	40	0.0052779033	14.250447	10
TGAAGCG	100	1.0084477E-8	13.298653	5
GTATAGG	130	1.4551915E-11	13.147278	1
CGCTTAC	60	4.0850884E-4	12.6691675	18
AACCGCG	115	5.320544E-9	12.391693	7
GCACCGT	70	1.0905374E-4	12.21467	6
ACAGCGT	70	1.0905374E-4	12.21467	8
CGCATCG	150	1.4551915E-11	12.03491	13
CGAACGA	80	2.861411E-5	11.87695	16
TTAGAAC	80	2.8740746E-5	11.871826	3
CGTCTTA	105	2.7148417E-7	11.763446	15
GCCGTTA	75	2.0712122E-4	11.401494	15
AGACCGT	75	2.073129E-4	11.400358	6
ACCGTGG	75	2.073129E-4	11.400358	8
GTCCTAA	100	1.9408872E-6	11.394306	1
CCGTCCA	110	4.965168E-7	11.227625	9
AAGCGTG	85	5.32067E-5	11.176823	7
GTATTAT	85	5.3495252E-5	11.17089	1
GCGTTAT	85	5.3495252E-5	11.17089	1
ACGGTAT	230	0.0	11.152524	9
>>END_MODULE