FastQCFastQC Report
Thu 2 Feb 2017
SRR4062699_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062699_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508270
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG450.001398429324.4442795
GACGCGA551.5939467E-423.99983837
TAGTACG1101.2653618E-720.001192
GTATTAG3000.019.0696981
TATTAGA2550.018.9815242
TCGATCG600.00741322318.33320841
CGATCGT600.00741322318.33320842
CAATACG4450.017.79763238
ATACGAA4400.017.49988240
CGCAATA4450.016.80887436
CGAATGC4750.016.6735743
TTAACGG2151.2732926E-1116.37198335
GTATTAT1753.619789E-916.3454551
CGTCTTA1906.166374E-1016.21041715
GTCCTAT4650.016.088581
GTTATAT2600.016.079481
AATACGA4800.016.04155739
ATAACGC4000.015.9509493
TACGACG4150.015.9035075
GTACGCC1259.443895E-615.8398924
Produced by FastQC (version 0.11.5)