##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062699_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1508270
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.228825077738072	32.0	32.0	32.0	32.0	32.0
2	31.29791814462928	32.0	32.0	32.0	32.0	32.0
3	31.427965152127936	32.0	32.0	32.0	32.0	32.0
4	31.557662752690167	32.0	32.0	32.0	32.0	32.0
5	31.452328827066772	32.0	32.0	32.0	32.0	32.0
6	35.055127397614484	36.0	36.0	36.0	36.0	36.0
7	35.07413924562578	36.0	36.0	36.0	36.0	36.0
8	35.01040861384235	36.0	36.0	36.0	36.0	36.0
9	35.16627659503935	36.0	36.0	36.0	36.0	36.0
10	35.01108886339979	36.0	36.0	36.0	36.0	36.0
11	35.165569825031326	36.0	36.0	36.0	36.0	36.0
12	35.062117525376756	36.0	36.0	36.0	36.0	36.0
13	35.11924124990884	36.0	36.0	36.0	36.0	36.0
14	35.07489971954624	36.0	36.0	36.0	36.0	36.0
15	35.02591246925285	36.0	36.0	36.0	36.0	36.0
16	35.04495481578232	36.0	36.0	36.0	36.0	36.0
17	35.01714746033535	36.0	36.0	36.0	36.0	36.0
18	35.03206919185557	36.0	36.0	36.0	36.0	36.0
19	35.01643803828227	36.0	36.0	36.0	36.0	36.0
20	35.00322223474577	36.0	36.0	36.0	36.0	36.0
21	34.99681688291884	36.0	36.0	36.0	36.0	36.0
22	34.98398761494958	36.0	36.0	36.0	36.0	36.0
23	34.93302326506527	36.0	36.0	36.0	32.0	36.0
24	34.91651096952137	36.0	36.0	36.0	32.0	36.0
25	34.88880770684294	36.0	36.0	36.0	32.0	36.0
26	34.84158738156961	36.0	36.0	36.0	32.0	36.0
27	34.82221949650925	36.0	36.0	36.0	32.0	36.0
28	34.81844629940262	36.0	36.0	36.0	32.0	36.0
29	34.783506268771504	36.0	36.0	36.0	32.0	36.0
30	34.774645123220644	36.0	36.0	36.0	32.0	36.0
31	34.77232325777215	36.0	36.0	36.0	32.0	36.0
32	34.72917315865197	36.0	36.0	36.0	32.0	36.0
33	34.70375529580247	36.0	36.0	36.0	32.0	36.0
34	34.694455236794475	36.0	36.0	36.0	32.0	36.0
35	34.66238538192764	36.0	36.0	36.0	32.0	36.0
36	34.62506447784548	36.0	36.0	36.0	32.0	36.0
37	34.628462410576354	36.0	36.0	36.0	32.0	36.0
38	34.58765671928766	36.0	36.0	36.0	32.0	36.0
39	34.56899891929164	36.0	36.0	36.0	32.0	36.0
40	34.54934129830866	36.0	36.0	36.0	32.0	36.0
41	34.52395260795481	36.0	36.0	36.0	32.0	36.0
42	34.483981647848196	36.0	36.0	36.0	32.0	36.0
43	34.483748930894336	36.0	36.0	36.0	32.0	36.0
44	34.45542310063848	36.0	36.0	36.0	32.0	36.0
45	34.39218840128094	36.0	36.0	36.0	32.0	36.0
46	34.42587998170089	36.0	36.0	36.0	32.0	36.0
47	34.38370583516214	36.0	36.0	36.0	32.0	36.0
48	34.35109430009216	36.0	36.0	36.0	32.0	36.0
49	34.34167423604527	36.0	36.0	36.0	32.0	36.0
50	33.77539830401719	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	30.0
22	107.0
23	349.0
24	937.0
25	2210.0
26	4880.0
27	8998.0
28	15306.0
29	23994.0
30	35229.0
31	51747.0
32	78967.0
33	130412.0
34	285912.0
35	869184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00541616547685	17.75856421219592	11.41419740888734	26.821822213439887
2	16.593831160866017	20.52257237634706	35.72772184992239	27.155874612864533
3	18.78790370114496	24.00682109154703	28.351023660992812	28.854251546315197
4	12.734465801524793	15.73903594120147	35.58828033991284	35.938217917360895
5	14.74331518892506	36.329834843894	33.21845558156033	15.708394385620611
6	34.61493835632333	35.0634669636967	16.55412013140927	13.76747454857071
7	30.171056906256837	30.196715442195366	20.898778070239413	18.73344958130839
8	28.552712710588953	32.24747558461018	19.552732600926888	19.647079103873974
9	27.708102660664203	13.870328256877084	18.231019645023768	40.19054943743494
10	15.797635701830574	26.695419255173146	31.675164261040795	25.831780781955487
11	37.95633407811599	20.993588681071692	21.598056050972307	19.452021189840014
12	24.413268181426403	23.650208517042703	28.516114488785167	23.420408812745727
13	29.919576733608704	19.215790276276795	24.94745635728351	25.91717663283099
14	23.768290823261086	19.05580565813813	24.834744442308075	32.341159076292705
15	25.57194666737388	26.43677856086775	22.287587766116147	25.70368700564223
16	26.31087272172754	25.33631246394876	23.315188925059836	25.037625889263857
17	24.489514476850964	25.30024465115662	25.018265960338663	25.191974911653748
18	25.419255173145395	24.53811320254331	25.618224853640264	24.424406770671034
19	25.705941243941734	25.1028661976967	24.989557572583156	24.20163498577841
20	25.734715932823697	24.037075589914274	25.113341775676766	25.11486670158526
21	26.75097959914339	24.241548263905006	24.027064119819396	24.98040801713221
22	25.844311694855698	24.284246189342756	24.586778229362118	25.28466388643943
23	24.82141791589039	24.032699715568167	25.06494195336379	26.080940415177658
24	24.84581673042625	24.954815782320143	24.765724969667236	25.433642517586375
25	25.259535759512552	24.192087623568725	24.909001703938948	25.639374912979772
26	24.78899666505334	24.762608816723798	25.182294947191153	25.266099571031713
27	25.82866462901205	24.574313617588363	24.46584497470612	25.13117677869347
28	24.917620850378245	24.22119381808297	25.31211255279227	25.549072778746513
29	24.73562425825615	24.909266908444774	25.23759008665557	25.11751874664351
30	25.08171613835719	24.6811247323092	25.303957514238167	24.93320161509544
31	25.039150815172352	24.62092330948703	24.620127695969554	25.719798179371068
32	25.12116530859859	24.65765413354373	24.603287209849693	25.617893348007982
33	24.800400458803797	24.27184787869546	24.952760447400003	25.974991215100747
34	25.109628912595227	24.603950221114257	25.36124168749627	24.92517917879425
35	25.879915399762645	24.315672923282968	24.9689379222553	24.83547375469909
36	24.86285611992548	24.8906362919106	24.829108846559304	25.417398741604618
37	25.79060778242622	24.59804942085966	24.69385454858878	24.917488248125334
38	24.98591101062807	24.3673215007923	25.019127874982594	25.627639613597037
39	25.24899388040603	24.483613676596367	24.77215617893348	25.495236264064125
40	25.536342962466936	24.50038786158977	24.997314804378526	24.965954371564774
41	24.439457126376578	24.850590411531094	25.428669933102167	25.281282528990168
42	25.660060864434087	24.858347643326457	25.023437448202245	24.458154044037208
43	25.038819309540067	23.885047106950346	25.11440259370007	25.961730989809517
44	24.728596338851798	24.453446664058824	25.230761070630592	25.58719592645879
45	24.871674169744143	24.690009083254324	25.079395598931224	25.35892114807031
46	24.618585436132992	24.368894897256254	25.432963792219816	25.57955587439094
47	24.817042041544283	24.169545240573637	25.73372141592686	25.27969130195522
48	25.15265834366526	25.253170851372765	24.787206534639022	24.806964270322954
49	24.304666936291248	24.75863074913643	25.433443614207007	25.503258700365315
50	24.343983504279738	25.276575149011784	24.820821205752285	25.558620140956194
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	9.0
2	9.0
3	52.5
4	96.0
5	131.5
6	167.0
7	171.5
8	176.0
9	251.0
10	326.0
11	461.5
12	597.0
13	1042.5
14	1488.0
15	2106.0
16	2724.0
17	3252.0
18	3780.0
19	4223.0
20	4666.0
21	5535.0
22	6404.0
23	7067.0
24	7730.0
25	8789.0
26	9848.0
27	12006.5
28	14165.0
29	16138.5
30	18112.0
31	19790.0
32	21468.0
33	24885.0
34	28302.0
35	32240.0
36	36178.0
37	43766.5
38	51355.0
39	56489.5
40	61624.0
41	68633.0
42	75642.0
43	78554.0
44	81466.0
45	90192.5
46	98919.0
47	105914.5
48	112910.0
49	115177.0
50	117444.0
51	114089.0
52	110734.0
53	108687.5
54	106641.0
55	107180.5
56	107720.0
57	105155.0
58	102590.0
59	93386.5
60	84183.0
61	74977.5
62	65772.0
63	58198.0
64	50624.0
65	43116.5
66	35609.0
67	31816.0
68	28023.0
69	25137.0
70	22251.0
71	17744.5
72	13238.0
73	11608.5
74	9979.0
75	7546.5
76	5114.0
77	4513.5
78	3913.0
79	3276.0
80	2639.0
81	2082.0
82	1525.0
83	1275.5
84	1026.0
85	816.0
86	606.0
87	466.0
88	326.0
89	213.0
90	100.0
91	63.5
92	27.0
93	18.0
94	9.0
95	9.0
96	9.0
97	7.5
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012729816279578591
2	0.0051051867371226635
3	4.641078851929694E-4
4	6.630112645613849E-5
5	0.0
6	3.315056322806925E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.9890337936841548E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1508270.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.93830337791864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.096066286406	48.60760623830403
2	11.982980816344426	14.364790790836626
3	3.2118058056084946	5.775305723025672
4	1.2118364072855503	2.905416728971532
5	0.6321032391214239	1.894359785631248
6	0.38560462573804016	1.3867492224849254
7	0.25370837686409825	1.0644794763399745
8	0.1905776505872167	0.9138320830358055
9	0.14974240075958828	0.8077774900739471
>10	0.740100501717811	8.247474900910083
>50	0.07328234314980679	3.03047300941789
>100	0.06561865160162764	8.028944272785681
>500	0.005347439850279893	2.103522896300708
>1k	0.0012254549656891423	0.8692673818818683
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	6.630112645613849E-5	0.0
6	6.630112645613849E-5	0.0	0.0	6.630112645613849E-5	0.0
7	6.630112645613849E-5	0.0	0.0	6.630112645613849E-5	0.0
8	6.630112645613849E-5	0.0	0.0	6.630112645613849E-5	0.0
9	6.630112645613849E-5	0.0	0.0	1.3260225291227698E-4	0.0
10	1.3260225291227698E-4	0.0	0.0	1.3260225291227698E-4	0.0
11	1.3260225291227698E-4	0.0	0.0	1.3260225291227698E-4	0.0
12	1.3260225291227698E-4	0.0	0.0	1.3260225291227698E-4	3.3150563228069243E-4
13	1.3260225291227698E-4	0.0	0.0	1.3260225291227698E-4	3.3150563228069243E-4
14	1.3260225291227698E-4	0.0	0.0	1.3260225291227698E-4	3.3150563228069243E-4
15	1.9890337936841548E-4	0.0	0.0	1.3260225291227698E-4	5.304090116491079E-4
16	1.9890337936841548E-4	0.0	0.0	1.9890337936841548E-4	5.304090116491079E-4
17	1.9890337936841548E-4	0.0	0.0	2.6520450582455396E-4	5.304090116491079E-4
18	1.9890337936841548E-4	0.0	0.0	3.3150563228069243E-4	5.967101381052464E-4
19	1.9890337936841548E-4	0.0	0.0	3.3150563228069243E-4	5.967101381052464E-4
20	1.9890337936841548E-4	0.0	0.0	6.630112645613849E-4	6.630112645613849E-4
21	1.9890337936841548E-4	0.0	0.0	7.293123910175233E-4	7.956135174736619E-4
22	1.9890337936841548E-4	0.0	0.0	9.945168968420774E-4	7.956135174736619E-4
23	1.9890337936841548E-4	0.0	0.0	0.0017238292878596008	7.956135174736619E-4
24	1.9890337936841548E-4	0.0	0.0	0.002718346184701678	7.956135174736619E-4
25	1.9890337936841548E-4	0.0	0.0	0.003248755196350786	7.956135174736619E-4
26	1.9890337936841548E-4	0.0	0.0	0.00437587434610514	9.282157703859389E-4
27	1.9890337936841548E-4	0.0	0.0	0.005635595748771771	9.282157703859389E-4
28	1.9890337936841548E-4	0.0	0.0	0.01140379375045582	9.282157703859389E-4
29	1.9890337936841548E-4	0.0	0.0	0.023536899891929162	9.282157703859389E-4
30	1.9890337936841548E-4	0.0	0.0	0.04561517500182328	9.282157703859389E-4
31	1.9890337936841548E-4	0.0	0.0	0.09812566715508496	9.945168968420774E-4
32	1.9890337936841548E-4	0.0	0.0	0.15872489673599555	0.0010608180232982158
33	1.9890337936841548E-4	0.0	0.0	0.21799810378778336	0.0010608180232982158
34	1.9890337936841548E-4	0.0	0.0	0.29046523500434274	0.0011271191497543543
35	1.9890337936841548E-4	0.0	0.0	0.3725460295570422	0.0011271191497543543
36	1.9890337936841548E-4	0.0	0.0	0.48605355804995126	0.0011271191497543543
37	1.9890337936841548E-4	0.0	0.0	0.6543921181220869	0.0011271191497543543
38	1.9890337936841548E-4	0.0	0.0	0.8747770624622913	0.0011271191497543543
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	45	0.0013984293	24.444279	5
GACGCGA	55	1.5939467E-4	23.999838	37
TAGTACG	110	1.2653618E-7	20.00119	2
GTATTAG	300	0.0	19.069698	1
TATTAGA	255	0.0	18.981524	2
TCGATCG	60	0.007413223	18.333208	41
CGATCGT	60	0.007413223	18.333208	42
CAATACG	445	0.0	17.797632	38
ATACGAA	440	0.0	17.499882	40
CGCAATA	445	0.0	16.808874	36
CGAATGC	475	0.0	16.67357	43
TTAACGG	215	1.2732926E-11	16.371983	35
GTATTAT	175	3.619789E-9	16.345455	1
CGTCTTA	190	6.166374E-10	16.210417	15
GTCCTAT	465	0.0	16.08858	1
GTTATAT	260	0.0	16.07948	1
AATACGA	480	0.0	16.041557	39
ATAACGC	400	0.0	15.950949	3
TACGACG	415	0.0	15.903507	5
GTACGCC	125	9.443895E-6	15.839892	4
>>END_MODULE