FastQCFastQC Report
Thu 2 Feb 2017
SRR4062698_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062698_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901234
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20780.2305727480321426No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14990.16632750206938487No Hit
GTACATGGAAGCAGTGGTATCAACG14840.16466311745895074No Hit
TATCAACGCAGAGTACTTTTTTTTT13580.15068228673130396No Hit
TCCATGTACTCTGCGTTGATACCAC12190.13525898934128097No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11700.12982199961386276No Hit
GCGCAAGACGGACCAGAGCGAAAGC10760.11939185605514216No Hit
GTCTTGCGCCGGTCCAAGAATTTCA9300.10319184584691656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC504.573769E-617.0938013
CGCGCTA456.7513756E-414.77980916
AATCGTA456.759915E-414.77734613
GCGTTAT801.2973396E-714.2400891
CGCGTAA551.9564258E-413.82008210
TCGCGTA551.9573484E-413.8193149
GGACCGA500.001497140413.3018286
TCTTATA802.0061325E-613.057042
CAAGACG2000.012.8224874
TTTTCGG1207.366907E-1012.66840816
GCGTAAC604.086229E-412.66770411
CGTTATT905.423917E-712.6613722
ATCCCCG1155.298716E-912.39300712
CGACGGG853.9393944E-612.29375714
AAGACGG2100.012.2139285
CTAGCAC701.09361186E-412.2098593
GCGCAAG2100.012.205791
GTCCTAC1950.012.1710161
TTCGGAA1251.3988029E-912.16369918
CGACAAT550.003062288712.09324417
Produced by FastQC (version 0.11.5)