##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062698_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	901234
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.140617198197138	32.0	32.0	32.0	32.0	32.0
2	30.83495629326013	32.0	32.0	32.0	32.0	32.0
3	30.819032570897235	32.0	32.0	32.0	32.0	32.0
4	30.88215602163256	32.0	32.0	32.0	32.0	32.0
5	30.694421204703772	32.0	32.0	32.0	32.0	32.0
6	34.32116409278833	36.0	36.0	36.0	32.0	36.0
7	34.1992911940739	36.0	36.0	36.0	32.0	36.0
8	34.20380833390662	36.0	36.0	36.0	32.0	36.0
9	34.350345193368206	36.0	36.0	36.0	32.0	36.0
10	34.04317746556388	36.0	36.0	36.0	32.0	36.0
11	34.338181870635154	36.0	36.0	36.0	32.0	36.0
12	34.116937443549624	36.0	36.0	36.0	32.0	36.0
13	34.238473026983	36.0	36.0	36.0	32.0	36.0
14	34.15874234660477	36.0	36.0	36.0	32.0	36.0
15	34.09395451125901	36.0	36.0	36.0	32.0	36.0
16	34.10017930970203	36.0	36.0	36.0	32.0	36.0
17	34.02769425032788	36.0	36.0	36.0	32.0	36.0
18	34.037822585477244	36.0	36.0	36.0	32.0	36.0
19	34.02649034545967	36.0	36.0	36.0	32.0	36.0
20	34.00627140121212	36.0	36.0	36.0	32.0	36.0
21	33.975826477918055	36.0	36.0	36.0	32.0	36.0
22	33.942119360787544	36.0	36.0	36.0	32.0	36.0
23	33.90278551408402	36.0	36.0	36.0	32.0	36.0
24	33.87788853949141	36.0	36.0	36.0	32.0	36.0
25	33.44311133401536	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	6.0
4	43.0
5	88.0
6	128.0
7	47.0
8	161.0
9	141.0
10	107.0
11	33.0
12	69.0
13	56.0
14	159.0
15	239.0
16	320.0
17	475.0
18	650.0
19	822.0
20	1318.0
21	1798.0
22	2732.0
23	4022.0
24	5542.0
25	7668.0
26	10760.0
27	13858.0
28	18562.0
29	24945.0
30	32232.0
31	44269.0
32	63196.0
33	90608.0
34	201299.0
35	374880.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.37277260793239	17.583774798427815	11.003965972972793	26.039486620667002
2	16.97149927042127	20.465044024389567	36.431555528431375	26.131901176757786
3	19.450880393436222	23.967141151457856	28.227739384013102	28.354239071092824
4	12.72232721816727	15.305079497898664	35.96465625634646	36.00793702758761
5	14.652642421713011	36.55368536023336	33.43208467826079	15.361587539792835
6	33.95526198934281	35.33503552397869	16.931172291296626	13.778530195381883
7	29.778542678695352	30.621401804302568	21.025399028452462	18.574656488549618
8	28.14166898961282	32.508881798504504	19.969636965162472	19.379812246720203
9	27.363811241625648	14.33014056474686	18.85784479235157	39.44820340127592
10	15.826080967288256	26.704296763041484	31.941886274309404	25.527735995360857
11	37.37713622992633	21.356300489677665	22.161358122339433	19.105205158056577
12	24.32328834699367	24.027638433822084	28.92458316250968	22.724490056674565
13	29.406223268938092	19.688947643222946	25.379983780841396	25.52484530699757
14	23.18440315909573	20.02997341498071	25.23677109220769	31.54885233371587
15	25.029270947889938	27.280079092656155	22.71103409204519	24.979615867408718
16	25.443495802202538	25.913706880655475	24.170826696170106	24.47197062097188
17	23.709176792621772	25.7809149908572	25.727591255796177	24.78231696072485
18	24.536630185170168	24.901815483101153	26.71718748437658	23.8443668473521
19	25.08974311969416	25.430494773178502	25.672142913487743	23.8076191936396
20	25.243930753994043	24.555047864085328	25.572319372373364	24.628702009547265
21	26.157649150094436	24.539051216531497	24.683812909676703	24.619486723697367
22	25.285610307640166	24.885350283082293	25.151486529283325	24.67755287999422
23	23.98543542609268	24.60977430910497	25.737895447959534	25.666894816842817
24	24.2196110498809	25.591513492373874	25.55118302698475	24.63769243076048
25	24.504182410655066	24.94436599454048	25.647415393537678	24.904036201266777
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	158.0
1	158.0
2	393.5
3	629.0
4	629.0
5	629.0
6	1621.5
7	2614.0
8	2614.0
9	2614.0
10	2756.5
11	2899.0
12	2899.0
13	2899.0
14	3079.0
15	3259.0
16	3259.0
17	3259.0
18	5105.5
19	6952.0
20	6952.0
21	6952.0
22	10943.5
23	14935.0
24	14935.0
25	14935.0
26	21779.5
27	28624.0
28	28624.0
29	28624.0
30	35351.0
31	42078.0
32	42078.0
33	42078.0
34	50602.5
35	59127.0
36	59127.0
37	59127.0
38	65599.0
39	72071.0
40	72071.0
41	72071.0
42	82026.0
43	91981.0
44	91981.0
45	91981.0
46	104588.0
47	117195.0
48	117195.0
49	117195.0
50	117203.0
51	117211.0
52	117211.0
53	117211.0
54	108569.0
55	99927.0
56	99927.0
57	99927.0
58	91942.5
59	83958.0
60	83958.0
61	83958.0
62	74599.0
63	65240.0
64	65240.0
65	65240.0
66	54101.0
67	42962.0
68	42962.0
69	42962.0
70	32765.5
71	22569.0
72	22569.0
73	22569.0
74	17649.0
75	12729.0
76	12729.0
77	12729.0
78	10437.0
79	8145.0
80	8145.0
81	8145.0
82	5729.5
83	3314.0
84	3314.0
85	3314.0
86	2491.0
87	1668.0
88	1668.0
89	1668.0
90	1099.0
91	530.0
92	530.0
93	530.0
94	306.0
95	82.0
96	82.0
97	82.0
98	229.5
99	377.0
100	377.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007545210233968092
2	0.003217810246839334
3	0.005104112805331356
4	0.0156452153380809
5	0.035284953741203724
6	0.04815619472856106
7	0.067574015183626
8	0.0884343023010672
9	0.09609047150906423
10	0.11850418426291062
11	0.11650692273038968
12	0.13126446627623903
13	0.11806034836679487
14	0.12238774835392362
15	0.11362198940563717
16	0.11095897402894254
17	0.11894802015902639
18	0.12682610731508132
19	0.12926720474371806
20	0.11994665092528688
21	0.12582747654882084
22	0.1463548867441752
23	0.13781104574394665
24	0.12982199961386276
25	0.12815761500342865
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	901234.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.76810645251489
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.89350641635262	50.14156022167305
2	10.663826144733186	12.74713392419049
3	2.5233892398118383	4.524545901186137
4	0.9349439629046339	2.2351932120808113
5	0.45358054673716236	1.3554825201088312
6	0.2882254979456154	1.0336015346125593
7	0.18367488881946334	0.7684530215330865
8	0.13443040660637626	0.6427720682003805
9	0.088260175342092	0.47476291998274067
>10	0.6440798845748376	7.712511515407453
>50	0.09000033544031905	3.787597175665668
>100	0.09520262366138871	11.147334530987543
>500	0.0055782787057193466	2.3305991913667325
>1k	0.0013015983646678477	1.0984522630045674
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2078	0.2305727480321426	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1499	0.16632750206938487	No Hit
GTACATGGAAGCAGTGGTATCAACG	1484	0.16466311745895074	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1358	0.15068228673130396	No Hit
TCCATGTACTCTGCGTTGATACCAC	1219	0.13525898934128097	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1170	0.12982199961386276	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1076	0.11939185605514216	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	930	0.10319184584691656	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2191794805788507E-4
2	0.0	0.0	0.0	0.0	2.2191794805788507E-4
3	0.0	0.0	0.0	0.0	2.2191794805788507E-4
4	0.0	0.0	0.0	0.0	2.2191794805788507E-4
5	0.0	0.0	0.0	0.0	2.2191794805788507E-4
6	0.0	0.0	0.0	0.0	3.328769220868276E-4
7	0.0	0.0	0.0	0.0	3.328769220868276E-4
8	0.0	0.0	0.0	1.1095897402894253E-4	3.328769220868276E-4
9	0.0	0.0	0.0	1.1095897402894253E-4	3.328769220868276E-4
10	0.0	0.0	0.0	1.1095897402894253E-4	3.328769220868276E-4
11	0.0	0.0	0.0	1.1095897402894253E-4	3.328769220868276E-4
12	0.0	0.0	0.0	1.1095897402894253E-4	7.767128182025977E-4
13	0.0	0.0	0.0	1.1095897402894253E-4	8.876717922315403E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGGAC	50	4.573769E-6	17.093801	3
CGCGCTA	45	6.7513756E-4	14.779809	16
AATCGTA	45	6.759915E-4	14.777346	13
GCGTTAT	80	1.2973396E-7	14.240089	1
CGCGTAA	55	1.9564258E-4	13.820082	10
TCGCGTA	55	1.9573484E-4	13.819314	9
GGACCGA	50	0.0014971404	13.301828	6
TCTTATA	80	2.0061325E-6	13.05704	2
CAAGACG	200	0.0	12.822487	4
TTTTCGG	120	7.366907E-10	12.668408	16
GCGTAAC	60	4.086229E-4	12.667704	11
CGTTATT	90	5.423917E-7	12.661372	2
ATCCCCG	115	5.298716E-9	12.393007	12
CGACGGG	85	3.9393944E-6	12.293757	14
AAGACGG	210	0.0	12.213928	5
CTAGCAC	70	1.09361186E-4	12.209859	3
GCGCAAG	210	0.0	12.20579	1
GTCCTAC	195	0.0	12.171016	1
TTCGGAA	125	1.3988029E-9	12.163699	18
CGACAAT	55	0.0030622887	12.093244	17
>>END_MODULE