FastQCFastQC Report
Thu 2 Feb 2017
SRR4062698_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062698_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901234
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10360.11495349709398446No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC9620.10674253301584272No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9140.10141650226245348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG2000.025.299844
AGGACGT2150.022.5114865
AAATTCG703.214339E-521.99986321
TAGGACA1551.8189894E-1221.290194
GTAGTAA956.848113E-720.8466011
TACGACG3400.019.4116425
TTAGGAC1851.8189894E-1219.0269073
CGACGGT3400.018.7645877
ACGACGG3450.018.4926366
GGACGTG2750.018.3998836
AATTCGT600.007410944418.3332222
GGACCGA600.007410944418.333226
ACGGTAT3750.018.1865529
GTAGGAC3850.017.7141743
CTACGAC3750.017.5998884
GACGGTA3650.017.4793428
CTTTCGT3650.017.47934238
TGTAGGA3300.017.3341852
TTCGTTC3700.017.24313540
CGACTTT4000.017.04989235
Produced by FastQC (version 0.11.5)