##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062698_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	901234
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.227089745837375	32.0	32.0	32.0	32.0	32.0
2	31.366688340652928	32.0	32.0	32.0	32.0	32.0
3	31.45603361613077	32.0	32.0	32.0	32.0	32.0
4	31.56851272810391	32.0	32.0	32.0	32.0	32.0
5	31.49113548756483	32.0	32.0	32.0	32.0	32.0
6	35.085202067387605	36.0	36.0	36.0	36.0	36.0
7	35.081693544628806	36.0	36.0	36.0	36.0	36.0
8	35.02909899093909	36.0	36.0	36.0	36.0	36.0
9	35.160380101061435	36.0	36.0	36.0	36.0	36.0
10	35.03270071923607	36.0	36.0	36.0	36.0	36.0
11	35.18159878566499	36.0	36.0	36.0	36.0	36.0
12	35.07764243248701	36.0	36.0	36.0	36.0	36.0
13	35.12275391296822	36.0	36.0	36.0	36.0	36.0
14	35.088132493891706	36.0	36.0	36.0	36.0	36.0
15	35.05189218338412	36.0	36.0	36.0	36.0	36.0
16	35.05693526875373	36.0	36.0	36.0	36.0	36.0
17	35.029530621348066	36.0	36.0	36.0	36.0	36.0
18	35.04729182432087	36.0	36.0	36.0	36.0	36.0
19	35.02392386439038	36.0	36.0	36.0	36.0	36.0
20	35.019923793376634	36.0	36.0	36.0	36.0	36.0
21	35.00728556623474	36.0	36.0	36.0	36.0	36.0
22	34.98597811445196	36.0	36.0	36.0	36.0	36.0
23	34.959008426224486	36.0	36.0	36.0	32.0	36.0
24	34.93313501266042	36.0	36.0	36.0	32.0	36.0
25	34.90281658259675	36.0	36.0	36.0	32.0	36.0
26	34.86680928593462	36.0	36.0	36.0	32.0	36.0
27	34.84366213436244	36.0	36.0	36.0	32.0	36.0
28	34.83372242946893	36.0	36.0	36.0	32.0	36.0
29	34.802964601868105	36.0	36.0	36.0	32.0	36.0
30	34.786101056995186	36.0	36.0	36.0	32.0	36.0
31	34.79281629410342	36.0	36.0	36.0	32.0	36.0
32	34.743276440968714	36.0	36.0	36.0	32.0	36.0
33	34.73035526844305	36.0	36.0	36.0	32.0	36.0
34	34.716121451254615	36.0	36.0	36.0	32.0	36.0
35	34.676037521886656	36.0	36.0	36.0	32.0	36.0
36	34.65085649232053	36.0	36.0	36.0	32.0	36.0
37	34.64479258438985	36.0	36.0	36.0	32.0	36.0
38	34.59339749720938	36.0	36.0	36.0	32.0	36.0
39	34.5891677411194	36.0	36.0	36.0	32.0	36.0
40	34.5708284418919	36.0	36.0	36.0	32.0	36.0
41	34.544494548585604	36.0	36.0	36.0	32.0	36.0
42	34.5031534540419	36.0	36.0	36.0	32.0	36.0
43	34.50081000051041	36.0	36.0	36.0	32.0	36.0
44	34.468244651222655	36.0	36.0	36.0	32.0	36.0
45	34.41707370116973	36.0	36.0	36.0	32.0	36.0
46	34.41449057625434	36.0	36.0	36.0	32.0	36.0
47	34.3772383199036	36.0	36.0	36.0	32.0	36.0
48	34.333357374444375	36.0	36.0	36.0	32.0	36.0
49	34.331375647168215	36.0	36.0	36.0	32.0	36.0
50	33.79700943373197	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	20.0
22	73.0
23	222.0
24	646.0
25	1405.0
26	3004.0
27	5769.0
28	9738.0
29	15105.0
30	21523.0
31	30713.0
32	45275.0
33	73140.0
34	158930.0
35	535665.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69177262038328	18.307159796737547	11.53455605975357	26.466511523125597
2	16.401648476025194	21.055796963146754	35.99161558639852	26.550938974429535
3	18.846153419386194	24.280177402191896	28.65043179924303	28.22323737917888
4	12.456934000419425	15.80456996137514	36.109640903073895	35.62885513513154
5	14.320919983045469	36.68037379859171	33.492966310636305	15.505739907726518
6	34.04546881882931	35.68688674293995	16.672713937461232	13.5949305007695
7	29.696094128821294	30.637138231733633	21.10530795033027	18.561459689114802
8	28.078390295971968	32.589205467170565	19.90748240745467	19.4249218294028
9	27.434162492759928	14.03431295312871	18.789459785139044	39.74206476897232
10	15.762055137733375	26.786605920327016	31.83590499248808	25.61543394945153
11	37.54030584731601	21.229891459931604	22.12854819059201	19.101254502160373
12	24.387229065925165	24.000315123486242	28.91324561656573	22.69921019402286
13	29.594755635051495	19.41826429096106	25.43279547819989	25.554184595787554
14	23.374728427911066	19.86320977681712	25.179254222543758	31.58280757272806
15	25.13276241242563	27.222452770312707	22.620207404514257	25.024577412747412
16	25.602784626412227	25.791082005339344	24.135130276931406	24.471003091317016
17	23.89978629301602	25.93166702543402	25.53898321634559	24.629563465204377
18	24.787790962169648	24.771258075039334	26.639030484868524	23.801920477922494
19	25.2691309915072	25.283888535053052	25.604338052048636	23.842642421391112
20	25.40017353983538	24.487092142551216	25.48916263700659	24.623571680606812
21	26.44152351109701	24.453138696498357	24.724766264921207	24.380571527483426
22	25.335817334898596	24.836280033820294	25.204663827596384	24.623238803684725
23	24.12991520515205	24.52437435782494	25.761566918247645	25.58414351877537
24	24.37835234800285	25.365665298912383	25.561285970125407	24.694696382959364
25	24.696027890647713	24.628675793412143	25.65648876984224	25.018807546097904
26	24.080538461709168	25.500702370305607	26.151143876063266	24.267615291921967
27	25.086159643333474	25.01758699738359	25.375651606574984	24.520601752707954
28	24.133354933346947	24.90207870541946	26.095664389048796	24.868901972184805
29	24.043034328487384	25.217202191661652	25.934662917732798	24.805100562118163
30	24.19882072802402	25.270684417143606	26.17488909650546	24.355605758326917
31	24.373692071093632	25.10113910482738	25.322058422119003	25.20311040195998
32	24.31754683023499	25.23140494033736	25.36433379122403	25.086714438203618
33	23.95748495951107	24.742297782817783	25.904260158848867	25.39595709882228
34	24.502848316863325	25.11378842786668	26.133945235088778	24.249418020181217
35	25.289658401702553	24.934922561732026	25.734381969610553	24.041037066954864
36	24.053797348968192	25.470077693473613	25.662480554439803	24.81364440311839
37	25.107574725321058	25.151514479036518	25.30485978114452	24.4360510144979
38	24.20814128184245	24.88532390034109	25.801955984794184	25.104578833022277
39	24.633668947243446	24.80964988005335	25.472962626798367	25.083718545904837
40	24.947239007849237	25.00183082307148	25.935661548499056	24.115268620580228
41	23.804916370221274	25.124440489373455	26.492453680176293	24.578189460228973
42	25.096589786892192	25.477511944733557	25.83180394880797	23.594094319566285
43	24.12325766671031	24.35948932241793	26.215056245103934	25.302196765767825
44	23.70161356540033	25.106132258658683	25.9818204816951	25.210433694245886
45	23.888357518691038	25.346136519483288	26.08323698395755	24.682268977868123
46	23.589323083683038	24.919832141264088	26.11430549668566	25.376539278367215
47	23.979343877394772	24.71422516238846	26.51542218780028	24.791008772416486
48	24.353053701924253	25.6456147903874	25.56361610858001	24.437715399108335
49	23.444743540523326	25.368883109159217	26.1585781273232	25.027795222994246
50	23.373396920222717	25.761899795169736	25.743036769584815	25.121666515022735
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	35.0
2	36.0
3	120.5
4	205.0
5	227.0
6	249.0
7	219.0
8	189.0
9	257.5
10	326.0
11	492.0
12	658.0
13	1045.5
14	1433.0
15	1866.5
16	2300.0
17	2737.5
18	3175.0
19	3464.5
20	3754.0
21	4360.0
22	4966.0
23	5356.0
24	5746.0
25	6710.5
26	7675.0
27	9344.0
28	11013.0
29	12471.0
30	13929.0
31	15092.5
32	16256.0
33	18618.0
34	20980.0
35	24111.0
36	27242.0
37	31769.0
38	36296.0
39	38798.0
40	41300.0
41	44146.0
42	46992.0
43	48230.5
44	49469.0
45	54027.0
46	58585.0
47	61900.0
48	65215.0
49	66403.5
50	67592.0
51	64081.5
52	60571.0
53	59343.0
54	58115.0
55	59113.5
56	60112.0
57	58624.5
58	57137.0
59	52078.0
60	47019.0
61	41797.0
62	36575.0
63	32051.5
64	27528.0
65	23698.5
66	19869.0
67	17514.0
68	15159.0
69	13888.0
70	12617.0
71	9782.5
72	6948.0
73	6275.0
74	5602.0
75	4108.0
76	2614.0
77	2356.5
78	2099.0
79	1805.0
80	1511.0
81	1195.0
82	879.0
83	747.5
84	616.0
85	495.5
86	375.0
87	278.0
88	181.0
89	121.5
90	62.0
91	41.0
92	20.0
93	12.5
94	5.0
95	4.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014757543545849358
2	0.004216441013099817
3	5.547948701447127E-4
4	1.1095897402894255E-4
5	0.0
6	1.1095897402894255E-4
7	1.1095897402894255E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	901234.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.66218238088275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.71075871360799	53.92871770848665
2	10.279375046768482	13.088148979777406
3	2.3309020941871714	4.451709426963757
4	0.8737535722873365	2.2250023709961693
5	0.44897877088618576	1.4291484198650464
6	0.2501371476763829	0.9554566029364614
7	0.1581330902694728	0.7046968343231442
8	0.12762764986794503	0.650004377818925
9	0.09101601870621712	0.5214850544211327
>10	0.5682100223607994	7.093712461952572
>50	0.08020683157592885	3.5887362574456856
>100	0.07652327984888674	9.419542488895864
>500	0.0042026514789669026	1.8281466145860064
>1k	1.751104782902876E-4	0.11549240153125816
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1036	0.11495349709398446	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	962	0.10674253301584272	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	914	0.10141650226245348	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1095897402894253E-4
2	0.0	0.0	0.0	0.0	1.1095897402894253E-4
3	0.0	0.0	0.0	0.0	1.1095897402894253E-4
4	0.0	0.0	0.0	0.0	1.1095897402894253E-4
5	0.0	0.0	0.0	0.0	1.1095897402894253E-4
6	0.0	0.0	0.0	0.0	2.2191794805788507E-4
7	0.0	0.0	0.0	0.0	2.2191794805788507E-4
8	0.0	0.0	0.0	0.0	2.2191794805788507E-4
9	0.0	0.0	0.0	0.0	2.2191794805788507E-4
10	0.0	0.0	0.0	0.0	3.328769220868276E-4
11	0.0	0.0	0.0	0.0	3.328769220868276E-4
12	0.0	0.0	0.0	0.0	4.4383589611577014E-4
13	0.0	0.0	0.0	0.0	5.547948701447127E-4
14	0.0	0.0	0.0	0.0	5.547948701447127E-4
15	0.0	0.0	0.0	1.1095897402894253E-4	7.767128182025977E-4
16	0.0	0.0	0.0	2.2191794805788507E-4	7.767128182025977E-4
17	0.0	0.0	0.0	2.2191794805788507E-4	7.767128182025977E-4
18	0.0	0.0	0.0	2.2191794805788507E-4	7.767128182025977E-4
19	0.0	0.0	0.0	3.328769220868276E-4	7.767128182025977E-4
20	0.0	0.0	0.0	7.767128182025977E-4	9.98630766260483E-4
21	0.0	0.0	0.0	9.98630766260483E-4	9.98630766260483E-4
22	0.0	0.0	0.0	0.0015534256364051955	9.98630766260483E-4
23	0.0	0.0	0.0	0.002441097428636736	9.98630766260483E-4
24	0.0	0.0	0.0	0.0055479487014471265	9.98630766260483E-4
25	0.0	0.0	0.0	0.007212333311881265	9.98630766260483E-4
26	0.0	0.0	0.0	0.008876717922315404	0.001220548714318368
27	0.0	0.0	0.0	0.012871240987357334	0.0013315076883473104
28	0.0	0.0	0.0	0.021969876857730622	0.0013315076883473104
29	0.0	0.0	0.0	0.04271920500114288	0.0013315076883473104
30	0.0	0.0	0.0	0.08333018949573584	0.0013315076883473104
31	0.0	0.0	0.0	0.15667407132886688	0.0013315076883473104
32	0.0	0.0	0.0	0.24965769156512072	0.0013315076883473104
33	0.0	0.0	0.0	0.34619199897030073	0.0013315076883473104
34	0.0	0.0	0.0	0.4656948139994718	0.0015534256364051955
35	0.0	0.0	0.0	0.5944072238730452	0.0015534256364051955
36	0.0	0.0	0.0	0.7698333618128034	0.0015534256364051955
37	0.0	0.0	0.0	1.0304759918067894	0.0015534256364051955
38	0.0	0.0	0.0	1.3660159292703116	0.0015534256364051955
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	200	0.0	25.29984	4
AGGACGT	215	0.0	22.511486	5
AAATTCG	70	3.214339E-5	21.999863	21
TAGGACA	155	1.8189894E-12	21.29019	4
GTAGTAA	95	6.848113E-7	20.846601	1
TACGACG	340	0.0	19.411642	5
TTAGGAC	185	1.8189894E-12	19.026907	3
CGACGGT	340	0.0	18.764587	7
ACGACGG	345	0.0	18.492636	6
GGACGTG	275	0.0	18.399883	6
AATTCGT	60	0.0074109444	18.33322	22
GGACCGA	60	0.0074109444	18.33322	6
ACGGTAT	375	0.0	18.186552	9
GTAGGAC	385	0.0	17.714174	3
CTACGAC	375	0.0	17.599888	4
GACGGTA	365	0.0	17.479342	8
CTTTCGT	365	0.0	17.479342	38
TGTAGGA	330	0.0	17.334185	2
TTCGTTC	370	0.0	17.243135	40
CGACTTT	400	0.0	17.049892	35
>>END_MODULE