FastQCFastQC Report
Thu 2 Feb 2017
SRR4062697_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062697_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1724856
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG104840.6078188556030184No Hit
TCCATGTACTCTGCGTTGATACCAC85480.4955776018403855No Hit
GTATCAACGCAGAGTACTTTTTTTT59610.34559406698298295No Hit
GAGTACATGGAAGCAGTGGTATCAA45570.26419596766338754No Hit
CATGTACTCTGCGTTGATACCACTG38920.2256420246095906No Hit
TATCAACGCAGAGTACTTTTTTTTT37160.2154382742675331No Hit
GTACTTTTTTTTTTTTTTTTTTTTT33830.19613231481352644No Hit
GCTTCCATGTACTCTGCGTTGATAC30060.1742754177740055No Hit
CCCATGTACTCTGCGTTGATACCAC26860.15572314442480995No Hit
CATGGAAGCAGTGGTATCAACGCAG25820.14969365558632142No Hit
GTACATGGGAAGCAGTGGTATCAAC25310.14673688702129337No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24510.1420988186839945No Hit
GGTATCAACGCAGAGTACTTTTTTT24070.13954788109848013No Hit
GATATACACTGTTCTACAAATCCCG23560.13659111253345205No Hit
GCGTTGATACCACTGCTTCCATGTA22920.13288065786361294No Hit
GTATCAACGCAGAGTACATGGAAGC21660.12557570023236722No Hit
GTACATGGGGTGGTATCAACGCAAA21410.12412630387696132No Hit
TTGTAGAACAGTGTATATCAATGAG20960.1215173904372307No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20830.12076370433241963No Hit
GTGTATATCAATGAGTTACAATGAG19560.11340077084695767No Hit
GTTCTACAGTGTGGTTTTTATCATT17390.10082001048203444No Hit
GTGTATATCAATGAGTTACAATGAA17250.10000834852300713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTG400.005275184314.25171912
GGTTCGT500.00149731513.30276618
CCAACGA3400.013.1343219
AACCGCG1900.013.0008147
TCCAACG4450.012.81165118
AGGATCG604.0932707E-412.6663565
CGTCTTA853.934858E-612.29667415
CGCCAGT1700.012.29667418
TCGAACG853.961768E-612.2891793
GCGTGGG550.003068572112.09096411
GAACGTC951.0402855E-611.9997075
GTCCTAC9400.011.9169381
CGCGGTC2000.011.87574510
ATTAGCT1459.822543E-1111.7883663
CGAACGA1052.7130045E-711.7643516
CGACTTT1302.6084308E-911.69439913
ACGCGCG1302.6084308E-911.69439913
CAAGACG3250.011.6910044
AGAACCG2200.011.6588055
ATTACCG3100.011.64758215
Produced by FastQC (version 0.11.5)