##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062697_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1724856
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.980287050049395	32.0	32.0	32.0	32.0	32.0
2	30.60396404105618	32.0	32.0	32.0	32.0	32.0
3	30.61484147082423	32.0	32.0	32.0	32.0	32.0
4	30.662993316543528	32.0	32.0	32.0	32.0	32.0
5	30.4581101262946	32.0	32.0	32.0	32.0	32.0
6	34.07935908852681	36.0	36.0	36.0	32.0	36.0
7	33.909593612452284	36.0	36.0	36.0	32.0	36.0
8	33.89721460806003	36.0	36.0	36.0	32.0	36.0
9	34.06300757860366	36.0	36.0	36.0	32.0	36.0
10	33.71333375075948	36.0	36.0	36.0	27.0	36.0
11	34.103401675270284	36.0	36.0	36.0	32.0	36.0
12	33.83970661898732	36.0	36.0	36.0	32.0	36.0
13	33.96923337368453	36.0	36.0	36.0	32.0	36.0
14	33.86981985742578	36.0	36.0	36.0	32.0	36.0
15	33.817660140904515	36.0	36.0	36.0	32.0	36.0
16	33.80459876070814	36.0	36.0	36.0	32.0	36.0
17	33.71184377130613	36.0	36.0	36.0	27.0	36.0
18	33.74743630772656	36.0	36.0	36.0	32.0	36.0
19	33.70938269629465	36.0	36.0	36.0	27.0	36.0
20	33.67714696183334	36.0	36.0	36.0	27.0	36.0
21	33.62013002824584	36.0	36.0	36.0	27.0	36.0
22	33.58318259611237	36.0	36.0	36.0	27.0	36.0
23	33.522452309062324	36.0	36.0	36.0	27.0	36.0
24	33.49683973618667	36.0	36.0	36.0	27.0	36.0
25	32.97068392955702	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	11.0
4	70.0
5	137.0
6	288.0
7	84.0
8	259.0
9	297.0
10	179.0
11	67.0
12	125.0
13	115.0
14	559.0
15	616.0
16	869.0
17	1264.0
18	1791.0
19	2511.0
20	3815.0
21	5518.0
22	8003.0
23	11085.0
24	15094.0
25	19924.0
26	25967.0
27	32269.0
28	41976.0
29	54078.0
30	67908.0
31	90826.0
32	128097.0
33	177419.0
34	376681.0
35	656954.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.726635473378444	17.673954763934066	12.12728948878955	24.472120273897943
2	14.799717070286004	20.767214939616537	39.95141493845699	24.48165305164047
3	19.90165765011529	25.26184331898763	28.27612157067146	26.560377460225617
4	11.94753175184852	16.26992318325618	36.64711844436817	35.13542662052714
5	13.452351747492127	37.959636275272274	33.85685892762796	14.73115304960763
6	32.65286472547022	35.9188820323226	17.88160136236795	13.546651879839239
7	28.609659483818866	31.31889105988388	20.99787902555844	19.073570430738815
8	26.96222840514453	34.84874244582609	20.070964990411312	18.11806415861807
9	26.926165912854955	15.45522915527825	18.815349208062475	38.80325572380432
10	15.443918629090442	28.232202072523823	32.06549467822364	24.258384620162094
11	36.505758494955444	22.024313258410793	22.170234600190614	19.299693646443153
12	23.958588517218598	24.29191914149078	30.15335995179419	21.59613238949643
13	29.929748533066018	20.52453809500868	25.5504521389752	23.995261232950103
14	23.099114954865428	20.767360310947055	25.777237036181376	30.356287698006142
15	24.6956386480877	28.075883715855117	24.001597258658393	23.22688037739879
16	24.36722048296124	26.700945004915976	26.04550092457489	22.886333587547895
17	22.508072740866034	26.70520143977181	27.033973715631713	23.75275210373044
18	23.546819934136472	25.892910528121448	28.704155741173643	21.856113796568433
19	24.347056784914635	26.586780740765683	25.94403999017777	23.122122484141915
20	24.10095578410169	25.501661854659986	27.561479335072388	22.83590302616594
21	25.52320005944268	25.644987890876248	24.798161920927306	24.03365012875377
22	23.625362663419896	26.369847305418777	26.23096543223209	23.773824598929238
23	23.065856916691775	26.081345387856185	26.023348526304975	24.829449169147065
24	23.11331707883432	27.23911529083943	26.243701381632416	23.403866248693834
25	24.18414438052558	25.912856205773593	26.00712846917866	23.895870944522166
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	511.0
1	511.0
2	941.0
3	1371.0
4	1371.0
5	1371.0
6	3322.5
7	5274.0
8	5274.0
9	5274.0
10	5489.0
11	5704.0
12	5704.0
13	5704.0
14	6097.5
15	6491.0
16	6491.0
17	6491.0
18	10488.5
19	14486.0
20	14486.0
21	14486.0
22	23807.5
23	33129.0
24	33129.0
25	33129.0
26	49919.5
27	66710.0
28	66710.0
29	66710.0
30	86991.5
31	107273.0
32	107273.0
33	107273.0
34	118809.0
35	130345.0
36	130345.0
37	130345.0
38	140317.5
39	150290.0
40	150290.0
41	150290.0
42	169476.5
43	188663.0
44	188663.0
45	188663.0
46	222925.0
47	257187.0
48	257187.0
49	257187.0
50	238259.5
51	219332.0
52	219332.0
53	219332.0
54	192623.0
55	165914.0
56	165914.0
57	165914.0
58	150322.0
59	134730.0
60	134730.0
61	134730.0
62	118028.0
63	101326.0
64	101326.0
65	101326.0
66	83077.5
67	64829.0
68	64829.0
69	64829.0
70	48750.0
71	32671.0
72	32671.0
73	32671.0
74	25602.5
75	18534.0
76	18534.0
77	18534.0
78	15012.5
79	11491.0
80	11491.0
81	11491.0
82	8050.5
83	4610.0
84	4610.0
85	4610.0
86	3370.5
87	2131.0
88	2131.0
89	2131.0
90	1450.0
91	769.0
92	769.0
93	769.0
94	474.5
95	180.0
96	180.0
97	180.0
98	542.5
99	905.0
100	905.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007304957631245739
2	0.0026668892939468573
3	0.0037684305240553414
4	0.013450398178166758
5	0.03391587471649807
6	0.04632270751877258
7	0.06493295672218434
8	0.08528248155208319
9	0.09357302870500493
10	0.11763300820474289
11	0.1165314669746344
12	0.12998186515280116
13	0.11856062187220266
14	0.12285083508420414
15	0.11038602642771339
16	0.11027007471928092
17	0.12076370433241963
18	0.12772080683836795
19	0.12946008246485502
20	0.1207057284782034
21	0.12685116902512442
22	0.1466209353128609
23	0.13618528155393841
24	0.13079352711182846
25	0.12673521731669193
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1724856.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.19438941440871
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.86287896062666	34.859134729371505
2	14.836518870595611	14.00400898266225
3	5.139045865190312	7.276023953408954
4	2.255012737133463	4.2569579700291325
5	1.1618990692669506	2.7417558567611753
6	0.6650701437123655	1.8832547610154884
7	0.43001151248150177	1.4205891540911608
8	0.2766279044624879	1.0444228036875602
9	0.211812127232602	0.8996709613778732
>10	0.9535769673738013	8.1775654291842
>50	0.0934643909517237	3.1021565955293666
>100	0.09492489293830994	9.410539592589416
>500	0.011665852649061987	3.7360611577654317
>1k	0.007122310038374687	5.739414275713426
>5k	2.455968978749892E-4	0.8408542695066957
>10k+	1.227984489374946E-4	0.6075895073063752
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	10484	0.6078188556030184	No Hit
TCCATGTACTCTGCGTTGATACCAC	8548	0.4955776018403855	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5961	0.34559406698298295	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4557	0.26419596766338754	No Hit
CATGTACTCTGCGTTGATACCACTG	3892	0.2256420246095906	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3716	0.2154382742675331	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3383	0.19613231481352644	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3006	0.1742754177740055	No Hit
CCCATGTACTCTGCGTTGATACCAC	2686	0.15572314442480995	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2582	0.14969365558632142	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2531	0.14673688702129337	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2451	0.1420988186839945	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2407	0.13954788109848013	No Hit
GATATACACTGTTCTACAAATCCCG	2356	0.13659111253345205	No Hit
GCGTTGATACCACTGCTTCCATGTA	2292	0.13288065786361294	No Hit
GTATCAACGCAGAGTACATGGAAGC	2166	0.12557570023236722	No Hit
GTACATGGGGTGGTATCAACGCAAA	2141	0.12412630387696132	No Hit
TTGTAGAACAGTGTATATCAATGAG	2096	0.1215173904372307	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2083	0.12076370433241963	No Hit
GTGTATATCAATGAGTTACAATGAG	1956	0.11340077084695767	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1739	0.10082001048203444	No Hit
GTGTATATCAATGAGTTACAATGAA	1725	0.10000834852300713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.797585421623602E-5
2	0.0	0.0	0.0	0.0	5.797585421623602E-5
3	0.0	0.0	0.0	0.0	5.797585421623602E-5
4	0.0	5.797585421623602E-5	0.0	0.0	5.797585421623602E-5
5	0.0	5.797585421623602E-5	0.0	0.0	5.797585421623602E-5
6	0.0	5.797585421623602E-5	0.0	0.0	1.1595170843247204E-4
7	0.0	5.797585421623602E-5	0.0	0.0	1.1595170843247204E-4
8	0.0	5.797585421623602E-5	0.0	0.0	1.1595170843247204E-4
9	0.0	5.797585421623602E-5	0.0	0.0	1.1595170843247204E-4
10	0.0	5.797585421623602E-5	0.0	5.797585421623602E-5	1.1595170843247204E-4
11	0.0	5.797585421623602E-5	0.0	2.3190341686494409E-4	1.1595170843247204E-4
12	0.0	5.797585421623602E-5	0.0	2.898792710811801E-4	4.0583097951365214E-4
13	0.0	5.797585421623602E-5	0.0	3.4785512529741616E-4	4.0583097951365214E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGTTG	40	0.0052751843	14.251719	12
GGTTCGT	50	0.001497315	13.302766	18
CCAACGA	340	0.0	13.13432	19
AACCGCG	190	0.0	13.000814	7
TCCAACG	445	0.0	12.811651	18
AGGATCG	60	4.0932707E-4	12.666356	5
CGTCTTA	85	3.934858E-6	12.296674	15
CGCCAGT	170	0.0	12.296674	18
TCGAACG	85	3.961768E-6	12.289179	3
GCGTGGG	55	0.0030685721	12.090964	11
GAACGTC	95	1.0402855E-6	11.999707	5
GTCCTAC	940	0.0	11.916938	1
CGCGGTC	200	0.0	11.875745	10
ATTAGCT	145	9.822543E-11	11.788366	3
CGAACGA	105	2.7130045E-7	11.76435	16
CGACTTT	130	2.6084308E-9	11.694399	13
ACGCGCG	130	2.6084308E-9	11.694399	13
CAAGACG	325	0.0	11.691004	4
AGAACCG	220	0.0	11.658805	5
ATTACCG	310	0.0	11.647582	15
>>END_MODULE