FastQCFastQC Report
Thu 2 Feb 2017
SRR4062697_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062697_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1724856
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA36580.21207567472299135No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC33160.19224793258103864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA4900.021.9999336
GATATAC13150.021.0822241
ATACACT12450.020.6746314
AATACGA5250.020.53326639
ATACGAA5450.020.1834240
CTAGCGG5350.020.14946729
TCTAGCG5400.019.96289628
TACGAAT5500.019.59993641
CGAATGC5550.019.42335943
TAGCGGC5600.019.24993530
ACTGTTC13150.019.072188
CGTCGTA3000.019.06660310
TGTAGGA11900.019.0425072
CAATACG5700.018.91221838
GTAGGAC12100.018.5453933
CGGTCCA6300.018.1586710
ATACCGT3400.018.1175886
ATCGAGC851.4313177E-418.1175888
CCGTCGT3300.017.999949
GCAATAC6000.017.96660637
Produced by FastQC (version 0.11.5)