##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062697_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1724856
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.096299633128794	32.0	32.0	32.0	32.0	32.0
2	31.303206180689866	32.0	32.0	32.0	32.0	32.0
3	31.438714304266558	32.0	32.0	32.0	32.0	32.0
4	31.57227559865867	32.0	32.0	32.0	32.0	32.0
5	31.492228336742315	32.0	32.0	32.0	32.0	32.0
6	35.06243071885421	36.0	36.0	36.0	36.0	36.0
7	35.073169006572144	36.0	36.0	36.0	36.0	36.0
8	35.0162848376908	36.0	36.0	36.0	36.0	36.0
9	35.139616292606455	36.0	36.0	36.0	36.0	36.0
10	35.02887719322656	36.0	36.0	36.0	36.0	36.0
11	35.16186452666194	36.0	36.0	36.0	36.0	36.0
12	35.06445291664927	36.0	36.0	36.0	36.0	36.0
13	35.106517297675865	36.0	36.0	36.0	36.0	36.0
14	35.06114191561498	36.0	36.0	36.0	36.0	36.0
15	35.0268109337823	36.0	36.0	36.0	36.0	36.0
16	35.03950474706294	36.0	36.0	36.0	36.0	36.0
17	35.01088438687056	36.0	36.0	36.0	36.0	36.0
18	35.0203124202832	36.0	36.0	36.0	36.0	36.0
19	34.99791750731655	36.0	36.0	36.0	36.0	36.0
20	34.96938585018112	36.0	36.0	36.0	36.0	36.0
21	34.94276681647627	36.0	36.0	36.0	36.0	36.0
22	34.92236801217029	36.0	36.0	36.0	32.0	36.0
23	34.86249170945285	36.0	36.0	36.0	32.0	36.0
24	34.83872740680961	36.0	36.0	36.0	32.0	36.0
25	34.80457846915917	36.0	36.0	36.0	32.0	36.0
26	34.749773314410014	36.0	36.0	36.0	32.0	36.0
27	34.71505389435408	36.0	36.0	36.0	32.0	36.0
28	34.67275355160083	36.0	36.0	36.0	32.0	36.0
29	34.63156344645582	36.0	36.0	36.0	32.0	36.0
30	34.6109083888742	36.0	36.0	36.0	32.0	36.0
31	34.59806615740676	36.0	36.0	36.0	32.0	36.0
32	34.54542118298571	36.0	36.0	36.0	32.0	36.0
33	34.51125543233754	36.0	36.0	36.0	32.0	36.0
34	34.4762142462907	36.0	36.0	36.0	32.0	36.0
35	34.41068819657989	36.0	36.0	36.0	32.0	36.0
36	34.365490220632914	36.0	36.0	36.0	32.0	36.0
37	34.323314525966225	36.0	36.0	36.0	32.0	36.0
38	34.24941908194075	36.0	36.0	36.0	32.0	36.0
39	34.157296609108236	36.0	36.0	36.0	32.0	36.0
40	34.116946574090825	36.0	36.0	36.0	32.0	36.0
41	34.091919557342756	36.0	36.0	36.0	32.0	36.0
42	33.97720853219052	36.0	36.0	36.0	32.0	36.0
43	33.99380759901116	36.0	36.0	36.0	32.0	36.0
44	33.95428951750175	36.0	36.0	36.0	32.0	36.0
45	33.818091481259884	36.0	36.0	36.0	32.0	36.0
46	33.85015502743418	36.0	36.0	36.0	32.0	36.0
47	33.768744753185196	36.0	36.0	36.0	32.0	36.0
48	33.72633773486019	36.0	36.0	36.0	27.0	36.0
49	33.72461179368017	36.0	36.0	36.0	27.0	36.0
50	33.18278917196566	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	9.0
20	27.0
21	95.0
22	270.0
23	774.0
24	1977.0
25	4484.0
26	9243.0
27	16841.0
28	26813.0
29	37486.0
30	51014.0
31	68908.0
32	98578.0
33	144345.0
34	302534.0
35	961456.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00141016408233	18.338975149337074	12.721396340763366	24.93821834581723
2	14.423983947003311	21.087589366107235	39.441855718996024	25.046570967893427
3	19.462758457556838	25.347508881942062	28.715167945233432	26.47456471526766
4	11.892679425643141	16.646655276310824	36.77106022491218	34.689605073133855
5	13.364253806841733	37.93571053798725	33.81982833339614	14.880207321774874
6	33.011178969021	36.075626461724696	17.426019482319003	13.487175086935297
7	28.546705982071526	31.307403409216082	21.029432056278385	19.116458552434004
8	26.980223276609756	34.89282583589587	19.969667033074064	18.15728385442031
9	26.92781310439828	15.053140667974601	18.71443181343834	39.30461441418878
10	15.472016214686906	28.19226648485439	32.167496880899044	24.16822041955966
11	36.78423010384635	21.693695009902275	22.25461139944436	19.267463486807017
12	23.87300736989059	24.12926064552635	30.330010157369657	21.667721827213402
13	30.169417041190684	20.169451826703213	25.62405209478357	24.03707903732254
14	23.168136934329592	20.549483551090642	25.823141178162118	30.45923833641765
15	24.807056357168367	28.02419448348152	23.976436293812352	23.19231286553776
16	24.53207688062076	26.4511936068866	26.093714489789292	22.923015022703343
17	22.703982245474403	26.592886594591086	27.049388470689728	23.653742689244783
18	23.723371690158483	25.519405677923256	28.836610128613632	21.920612503304625
19	24.554339608639793	26.27216416906687	26.158821374074126	23.01467484821921
20	24.21831155760249	25.16911556674876	27.805335633815233	22.807237241833523
21	25.579294735328627	25.360725764933424	25.128416517088965	23.931562982648984
22	23.810161543920188	25.997010764956613	26.433452995496438	23.759374695626764
23	23.216256893329064	25.573381198198575	26.292339766334116	24.918022142138245
24	23.251274309275676	26.820093967264512	26.399537120779936	23.529094602679876
25	24.397978729818607	25.403743848761867	26.201027795943542	23.99724962547598
26	22.374679393526183	26.550564221013236	27.57030152082261	23.504454864637975
27	24.01388869563604	26.018056000037106	26.974599618750783	22.993455685576073
28	22.985570969402662	26.011098897531156	26.779105038333633	24.224225094732546
29	22.627222214492107	25.698087260617697	27.24847755406828	24.42621297082191
30	23.535298019081015	25.891320782720413	27.308772442453165	23.264608755745407
31	23.493845283316407	25.812705524403196	26.288049553122118	24.405399639158283
32	23.037807214051494	26.559318574999885	26.487892322605482	23.914981888343142
33	22.89083842361333	25.812647548548977	27.07675307387979	24.219760953957895
34	23.058968400840417	26.701011562704362	27.57006961740574	22.669950419049474
35	24.070067298371576	26.195925920772517	27.09060930303747	22.64339747781844
36	22.89217186826031	27.028459187317665	26.67271934584684	23.406649598575186
37	24.021193653267286	26.455309892535954	26.513053843335328	23.01044261086143
38	23.47500313069613	26.38991312898004	26.046232265186198	24.088851475137634
39	23.784826095627693	25.608920396833128	26.771452225577093	23.83480128196209
40	24.835116670609025	26.174184975441428	26.105367636486754	22.88533071746279
41	22.831355197187474	25.910104959486475	27.986046371407237	23.272493471918814
42	24.859466529379844	26.871576525808532	26.48394996451878	21.78500698029285
43	23.449899585820496	25.51818818498472	26.829022248813818	24.20288998038097
44	22.952118901519896	26.484123892081424	26.981788624673598	23.581968581725082
45	23.307568863719638	26.593350401424814	26.5461580560928	23.55292267876275
46	22.961537614198072	25.777602046318123	26.76533781989664	24.495522519587166
47	23.805581451437106	25.77218040230605	27.226562681174542	23.195675465082303
48	23.670613662821708	27.160991990055983	25.427687876553172	23.740706470569137
49	23.052707008585063	26.46070164697807	27.11032109347099	23.376270250965877
50	23.015094022396084	27.753521310486967	25.07526719637303	24.15611747074392
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	53.0
1	52.0
2	51.0
3	191.0
4	331.0
5	353.5
6	376.0
7	371.5
8	367.0
9	489.0
10	611.0
11	968.5
12	1326.0
13	2394.5
14	3463.0
15	4886.0
16	6309.0
17	7192.0
18	8075.0
19	8634.5
20	9194.0
21	10352.5
22	11511.0
23	12469.5
24	13428.0
25	15054.5
26	16681.0
27	22165.5
28	27650.0
29	32449.0
30	37248.0
31	40964.5
32	44681.0
33	50657.5
34	56634.0
35	63894.0
36	71154.0
37	76863.0
38	82572.0
39	84378.0
40	86184.0
41	89220.0
42	92256.0
43	94727.5
44	97199.0
45	108150.5
46	119102.0
47	127634.5
48	136167.0
49	133936.5
50	131706.0
51	120969.5
52	110233.0
53	104504.0
54	98775.0
55	99182.0
56	99589.0
57	95252.5
58	90916.0
59	82611.5
60	74307.0
61	65370.5
62	56434.0
63	48466.0
64	40498.0
65	35179.5
66	29861.0
67	25909.0
68	21957.0
69	20387.5
70	18818.0
71	14423.5
72	10029.0
73	9077.5
74	8126.0
75	5716.0
76	3306.0
77	3001.0
78	2696.0
79	2304.5
80	1913.0
81	1546.0
82	1179.0
83	1025.5
84	872.0
85	729.0
86	586.0
87	429.0
88	272.0
89	177.5
90	83.0
91	58.0
92	33.0
93	27.0
94	21.0
95	15.0
96	9.0
97	9.0
98	9.0
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01356634988659923
2	0.004000333940920285
3	4.6380683372988817E-4
4	2.898792710811801E-4
5	5.797585421623602E-5
6	5.797585421623602E-4
7	1.1595170843247204E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.1595170843247204E-4
47	0.0
48	0.0
49	0.0
50	5.797585421623602E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1724856.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.76508680773738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.94430209203075	38.27733216731529
2	15.01460420212628	15.544645798137708
3	5.0775349177755	7.885171073638995
4	2.2044591858317935	4.5645608447478745
5	1.1297940026455502	2.924194231090399
6	0.6830078969355664	2.1213577845143847
7	0.4195635150667639	1.5203119245153327
8	0.26796651901030705	1.1097048094508604
9	0.188714953135655	0.8791961337886747
>10	0.9016363486052987	8.282207725586312
>50	0.07748508372552308	2.794919257504642
>100	0.07855248596615605	8.463741960305292
>500	0.008890227040483098	3.085576245575276
>1k	0.0034885701044933683	2.5470800438289793
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3658	0.21207567472299135	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3316	0.19224793258103864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.797585421623602E-5	0.0
11	0.0	0.0	0.0	1.7392756264870808E-4	0.0
12	0.0	0.0	0.0	1.7392756264870808E-4	5.797585421623602E-5
13	0.0	0.0	0.0	2.3190341686494409E-4	5.797585421623602E-5
14	0.0	0.0	0.0	2.3190341686494409E-4	5.797585421623602E-5
15	0.0	0.0	0.0	3.4785512529741616E-4	5.797585421623602E-5
16	0.0	0.0	0.0	4.6380683372988817E-4	5.797585421623602E-5
17	0.0	0.0	0.0	6.377343963785962E-4	5.797585421623602E-5
18	0.0	0.0	0.0	6.957102505948323E-4	5.797585421623602E-5
19	0.0	0.0	0.0	9.855895216760123E-4	5.797585421623602E-5
20	0.0	0.0	0.0	0.0016233239180546085	2.3190341686494409E-4
21	0.0	0.0	0.0	0.002261058314433205	2.3190341686494409E-4
22	0.0	0.0	0.0	0.003304623690325453	2.3190341686494409E-4
23	0.0	0.0	0.0	0.006725199089083379	2.3190341686494409E-4
24	0.0	0.0	0.0	0.010957436446868607	2.3190341686494409E-4
25	0.0	0.0	0.0	0.013798253303464174	2.3190341686494409E-4
26	0.0	0.0	0.0	0.0172188287022221	2.3190341686494409E-4
27	0.0	0.0	0.0	0.020755355809412495	2.3190341686494409E-4
28	0.0	0.0	0.0	0.029335782233415427	2.898792710811801E-4
29	0.0	0.0	0.0	0.04017726697185156	2.898792710811801E-4
30	0.0	0.0	0.0	0.06012096082223675	2.898792710811801E-4
31	0.0	5.797585421623602E-5	0.0	0.10006632437722338	2.898792710811801E-4
32	0.0	5.797585421623602E-5	0.0	0.14401202187313028	3.4785512529741616E-4
33	0.0	5.797585421623602E-5	0.0	0.1852908300750903	3.4785512529741616E-4
34	0.0	5.797585421623602E-5	0.0	0.23039604465532196	3.4785512529741616E-4
35	0.0	5.797585421623602E-5	0.0	0.2820525307619883	3.4785512529741616E-4
36	0.0	5.797585421623602E-5	0.0	0.3563775758672028	3.4785512529741616E-4
37	0.0	5.797585421623602E-5	0.0	0.4658939644816727	3.4785512529741616E-4
38	0.0	5.797585421623602E-5	0.0	0.591237761297175	3.4785512529741616E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATA	490	0.0	21.99993	36
GATATAC	1315	0.0	21.082224	1
ATACACT	1245	0.0	20.674631	4
AATACGA	525	0.0	20.533266	39
ATACGAA	545	0.0	20.18342	40
CTAGCGG	535	0.0	20.149467	29
TCTAGCG	540	0.0	19.962896	28
TACGAAT	550	0.0	19.599936	41
CGAATGC	555	0.0	19.423359	43
TAGCGGC	560	0.0	19.249935	30
ACTGTTC	1315	0.0	19.07218	8
CGTCGTA	300	0.0	19.066603	10
TGTAGGA	1190	0.0	19.042507	2
CAATACG	570	0.0	18.912218	38
GTAGGAC	1210	0.0	18.545393	3
CGGTCCA	630	0.0	18.15867	10
ATACCGT	340	0.0	18.117588	6
ATCGAGC	85	1.4313177E-4	18.117588	8
CCGTCGT	330	0.0	17.99994	9
GCAATAC	600	0.0	17.966606	37
>>END_MODULE