##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062696_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	645146
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.162747657119475	32.0	32.0	32.0	32.0	32.0
2	30.82721275494229	32.0	32.0	32.0	32.0	32.0
3	30.811196225350542	32.0	32.0	32.0	32.0	32.0
4	30.897164362795397	32.0	32.0	32.0	32.0	32.0
5	30.66179593456365	32.0	32.0	32.0	32.0	32.0
6	34.29119765138434	36.0	36.0	36.0	32.0	36.0
7	34.183676253127196	36.0	36.0	36.0	32.0	36.0
8	34.18515963828343	36.0	36.0	36.0	32.0	36.0
9	34.35806313609633	36.0	36.0	36.0	32.0	36.0
10	34.02731474735951	36.0	36.0	36.0	32.0	36.0
11	34.3272174050525	36.0	36.0	36.0	32.0	36.0
12	34.11022156225103	36.0	36.0	36.0	32.0	36.0
13	34.241852231897894	36.0	36.0	36.0	32.0	36.0
14	34.15146803979254	36.0	36.0	36.0	32.0	36.0
15	34.07395535274186	36.0	36.0	36.0	32.0	36.0
16	34.070925030923235	36.0	36.0	36.0	32.0	36.0
17	33.986758036165455	36.0	36.0	36.0	32.0	36.0
18	33.99925443233005	36.0	36.0	36.0	32.0	36.0
19	33.99820040734966	36.0	36.0	36.0	32.0	36.0
20	33.98819802029308	36.0	36.0	36.0	32.0	36.0
21	33.95222631776373	36.0	36.0	36.0	32.0	36.0
22	33.90202992810929	36.0	36.0	36.0	32.0	36.0
23	33.85984102823237	36.0	36.0	36.0	32.0	36.0
24	33.83363610717574	36.0	36.0	36.0	32.0	36.0
25	33.34642701032014	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	25.0
5	56.0
6	111.0
7	27.0
8	76.0
9	117.0
10	85.0
11	28.0
12	44.0
13	30.0
14	96.0
15	112.0
16	222.0
17	245.0
18	392.0
19	495.0
20	772.0
21	1157.0
22	1682.0
23	2597.0
24	3772.0
25	5149.0
26	7269.0
27	9811.0
28	13459.0
29	18222.0
30	23959.0
31	33870.0
32	49415.0
33	70654.0
34	151610.0
35	249586.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.057720180748575	16.656306435486247	10.504809369162682	25.7811640146025
2	17.112369094933687	19.519813245205572	36.286977387292985	27.08084027256775
3	20.05673363095238	22.80552455357143	27.81001984126984	29.32772197420635
4	12.537361097331084	14.460759996031353	35.167179283659095	37.83469962297847
5	14.584777313119943	36.437754198589275	33.59119517334735	15.386273314943427
6	35.6427521353737	34.31640128277496	16.27551748308139	13.765329098769952
7	30.9536586954701	29.99032165003459	20.054037453973553	19.001982200521763
8	28.950046540490227	32.544368600682596	19.228513807012103	19.277071051815078
9	27.056505414714373	14.043969342476807	18.020758990908245	40.87876625190058
10	15.69258846004032	26.715467568402218	31.72581073174971	25.866133239807752
11	38.54290329939431	20.783499459179286	21.000448485469224	19.673148755957175
12	24.098332158943112	23.57637520060344	28.735724950566965	23.589567689886483
13	29.942968877301613	18.673774218828807	25.2394143252869	26.14384257858268
14	23.319387067839063	19.263848238145933	24.629953226211946	32.78681146780306
15	25.572052374641935	26.641673158151185	22.291964731842207	25.494309735364677
16	26.202439327167777	25.565141828564336	23.43988579231581	24.79253305195208
17	24.149629247973976	25.806917186816186	25.156044856494603	24.887408708715235
18	25.4579485086313	24.333078292394866	26.041335255710873	24.167637943262964
19	25.457993705108546	25.520073502480088	24.780392716798172	24.241540075613194
20	24.97780798788285	24.37117459371547	25.45253082051473	25.198486597886948
21	26.46180110968843	24.348737050401585	24.096845536026073	25.092616303883908
22	25.39980192302319	24.451795577108758	24.99883575444664	25.14956674542141
23	24.39256456184247	24.057920175827995	25.19968460232264	26.34983066000689
24	24.507207956690173	25.52429969206318	24.86636411045992	25.10212824078673
25	25.242192568312117	24.325259730383255	24.737941588212106	25.694606113092522
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	50.0
2	141.0
3	232.0
4	232.0
5	232.0
6	832.0
7	1432.0
8	1432.0
9	1432.0
10	1460.0
11	1488.0
12	1488.0
13	1488.0
14	1590.5
15	1693.0
16	1693.0
17	1693.0
18	2710.5
19	3728.0
20	3728.0
21	3728.0
22	5977.0
23	8226.0
24	8226.0
25	8226.0
26	12516.0
27	16806.0
28	16806.0
29	16806.0
30	20939.5
31	25073.0
32	25073.0
33	25073.0
34	30877.0
35	36681.0
36	36681.0
37	36681.0
38	42017.0
39	47353.0
40	47353.0
41	47353.0
42	56008.5
43	64664.0
44	64664.0
45	64664.0
46	76783.5
47	88903.0
48	88903.0
49	88903.0
50	88420.0
51	87937.0
52	87937.0
53	87937.0
54	82059.0
55	76181.0
56	76181.0
57	76181.0
58	70271.0
59	64361.0
60	64361.0
61	64361.0
62	57646.5
63	50932.0
64	50932.0
65	50932.0
66	42270.0
67	33608.0
68	33608.0
69	33608.0
70	25119.5
71	16631.0
72	16631.0
73	16631.0
74	13041.0
75	9451.0
76	9451.0
77	9451.0
78	7635.0
79	5819.0
80	5819.0
81	5819.0
82	3989.5
83	2160.0
84	2160.0
85	2160.0
86	1600.0
87	1040.0
88	1040.0
89	1040.0
90	699.0
91	358.0
92	358.0
93	358.0
94	207.0
95	56.0
96	56.0
97	56.0
98	169.5
99	283.0
100	283.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007905187352940265
2	0.0034100808189154084
3	0.004030095513263664
4	0.01395033062283576
5	0.03518583390426353
6	0.045571080034596825
7	0.06324149882352212
8	0.08463200577853695
9	0.09393222619376079
10	0.11764778825258158
11	0.11702777355823332
12	0.13020308581313378
13	0.11795779559975572
14	0.11904282131486515
15	0.11036261559398956
16	0.1094325935524672
17	0.12090286539790994
18	0.12477795723758653
19	0.1261729902998701
20	0.11935282866203929
21	0.12725801601497955
22	0.14756349725488493
23	0.13640323275661634
24	0.13299315193770092
25	0.12772302703574076
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	645146.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.755895931252276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.6714275633851	47.17006470907678
2	11.565610832524136	13.359644312492561
3	3.0652836478000802	5.311146100863325
4	1.249334595901927	2.8862575561689927
5	0.6064539434502285	1.751314542250448
6	0.35831862108047013	1.2417007793612072
7	0.24256641261223352	0.9806748338274551
8	0.17140627383560553	0.7919778330890371
9	0.1260823223789553	0.6553797741080589
>10	0.7497147411803676	8.419594371356043
>50	0.09474719929194489	3.9430995388719996
>100	0.0917863089038304	10.221678428204031
>500	0.0064600334712336185	2.528252897717604
>1k	8.075041839042023E-4	0.7392143226125073
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	2030	0.31465745738173995	No Hit
TCCATGTACTCTGCGTTGATACCAC	1567	0.2428907565109293	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1158	0.17949425401382013	No Hit
GAGTACATGGAAGCAGTGGTATCAA	948	0.1469434825605367	No Hit
CATGTACTCTGCGTTGATACCACTG	894	0.13857328418683523	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	894	0.13857328418683523	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	860	0.13330315928487504	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	816	0.12648299764704424	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	800	0.12400293886965122	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	792	0.1227629094809547	No Hit
TATCAACGCAGAGTACTTTTTTTTT	783	0.12136787641867113	No Hit
GAATAGGACCGCGGTTCTATTTTGT	749	0.11609775151671094	No Hit
GAATAACGCCGCCGCATCGCCAGTC	695	0.1077275531430095	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	660	0.10230242456746226	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5500367358706402E-4
2	0.0	0.0	0.0	0.0	1.5500367358706402E-4
3	0.0	0.0	0.0	0.0	1.5500367358706402E-4
4	0.0	0.0	0.0	0.0	1.5500367358706402E-4
5	0.0	0.0	0.0	0.0	1.5500367358706402E-4
6	0.0	0.0	0.0	0.0	1.5500367358706402E-4
7	0.0	0.0	0.0	0.0	1.5500367358706402E-4
8	0.0	0.0	0.0	0.0	1.5500367358706402E-4
9	0.0	0.0	0.0	1.5500367358706402E-4	1.5500367358706402E-4
10	0.0	0.0	0.0	1.5500367358706402E-4	1.5500367358706402E-4
11	0.0	0.0	0.0	3.1000734717412804E-4	1.5500367358706402E-4
12	0.0	0.0	0.0	3.1000734717412804E-4	6.200146943482561E-4
13	0.0	0.0	0.0	3.1000734717412804E-4	9.300220415223841E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCGC	35	0.002166747	16.287512	14
CGCGTAT	35	0.002166747	16.287512	7
ACGCGCG	35	0.002166747	16.287512	13
AAGACGG	110	0.0	15.541136	5
CAAGACG	120	0.0	15.037489	4
ACAATAC	65	3.3798788E-6	14.609056	3
GCCGTTA	40	0.0052653733	14.253786	16
TACGCGT	40	0.005284393	14.246042	5
GCGTTAT	60	2.5816946E-5	14.239411	1
CGCGTAA	55	1.9542301E-4	13.820779	10
CGCAAGA	110	1.8735591E-10	13.810057	2
AGGCCCG	120	5.0931703E-11	13.460863	10
CGCGCTA	50	0.0014948894	13.303534	16
CGCGGTC	50	0.0014957645	13.302501	10
GCACGCG	50	0.0014957645	13.302501	11
ACTGTTC	150	0.0	13.301468	8
CTAGAAC	50	0.0015027816	13.294242	3
ACGATCA	65	5.4371943E-5	13.155296	9
ATTATAC	65	5.4649892E-5	13.148151	3
CGTTATT	65	5.472954E-5	13.1461115	2
>>END_MODULE