FastQCFastQC Report
Thu 2 Feb 2017
SRR4062696_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062696_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645146
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC10090.15639870664934757No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8220.12741301968856664No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA8070.12508796458476065No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7990.12384793519606414No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC7200.11160264498268609No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7100.11005260824681545No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA6630.10276743558822343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG1600.024.74991229
TCTAGCG1650.023.99991428
CGCAATA1650.023.99991436
TAGCGGC1700.023.29403530
TACGAGC852.3492066E-723.29403514
ATACACT1900.023.1578144
CGGTCCA1750.022.6284910
GTACCGT500.002579198221.9999246
GCTATAC500.002579198221.9999243
TAGCACC500.002579198221.9999244
AATACGA1850.021.40532939
ATACCGT1601.8189894E-1220.6249286
GATATAC1950.020.310771
CAATACG1950.020.30762138
CGTCGTG550.00448147519.99993129
ATACGAA2000.019.7999340
CGAATGC1900.019.68414343
CTAGACA808.9805384E-519.2499314
CTAGGAC1152.0178231E-719.1303673
ACACGCT2650.019.0942739
Produced by FastQC (version 0.11.5)