##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062696_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	645146
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.167982131176508	32.0	32.0	32.0	32.0	32.0
2	31.218807835745707	32.0	32.0	32.0	32.0	32.0
3	31.387867242453645	32.0	32.0	32.0	32.0	32.0
4	31.529900208634945	32.0	32.0	32.0	32.0	32.0
5	31.42833095144355	32.0	32.0	32.0	32.0	32.0
6	35.02160286198783	36.0	36.0	36.0	36.0	36.0
7	35.038299857706626	36.0	36.0	36.0	36.0	36.0
8	34.96001215228801	36.0	36.0	36.0	36.0	36.0
9	35.132084830410484	36.0	36.0	36.0	36.0	36.0
10	34.96860865602515	36.0	36.0	36.0	36.0	36.0
11	35.13410452827732	36.0	36.0	36.0	36.0	36.0
12	35.02402556940599	36.0	36.0	36.0	36.0	36.0
13	35.08893335772058	36.0	36.0	36.0	36.0	36.0
14	35.04971277819284	36.0	36.0	36.0	36.0	36.0
15	34.99921568141165	36.0	36.0	36.0	36.0	36.0
16	35.01122381600444	36.0	36.0	36.0	36.0	36.0
17	34.964950569328494	36.0	36.0	36.0	32.0	36.0
18	34.99371615107278	36.0	36.0	36.0	36.0	36.0
19	34.97685485145998	36.0	36.0	36.0	36.0	36.0
20	34.971600226925375	36.0	36.0	36.0	32.0	36.0
21	34.959322385940546	36.0	36.0	36.0	32.0	36.0
22	34.932393597728264	36.0	36.0	36.0	32.0	36.0
23	34.87611331388554	36.0	36.0	36.0	32.0	36.0
24	34.855364522139176	36.0	36.0	36.0	32.0	36.0
25	34.83047868234477	36.0	36.0	36.0	32.0	36.0
26	34.78179357850781	36.0	36.0	36.0	32.0	36.0
27	34.76373410049818	36.0	36.0	36.0	32.0	36.0
28	34.73919391889588	36.0	36.0	36.0	32.0	36.0
29	34.70193258580228	36.0	36.0	36.0	32.0	36.0
30	34.69744987956214	36.0	36.0	36.0	32.0	36.0
31	34.68946099022547	36.0	36.0	36.0	32.0	36.0
32	34.65097202803707	36.0	36.0	36.0	32.0	36.0
33	34.62514221587052	36.0	36.0	36.0	32.0	36.0
34	34.60644567276245	36.0	36.0	36.0	32.0	36.0
35	34.5738142993989	36.0	36.0	36.0	32.0	36.0
36	34.53446506682209	36.0	36.0	36.0	32.0	36.0
37	34.53522458482266	36.0	36.0	36.0	32.0	36.0
38	34.48612717121396	36.0	36.0	36.0	32.0	36.0
39	34.43787762769978	36.0	36.0	36.0	32.0	36.0
40	34.422710518239285	36.0	36.0	36.0	32.0	36.0
41	34.39867874868634	36.0	36.0	36.0	32.0	36.0
42	34.34721132890849	36.0	36.0	36.0	32.0	36.0
43	34.345582240299095	36.0	36.0	36.0	32.0	36.0
44	34.31548672703543	36.0	36.0	36.0	32.0	36.0
45	34.24664184541174	36.0	36.0	36.0	32.0	36.0
46	34.279750630089936	36.0	36.0	36.0	32.0	36.0
47	34.24481590213688	36.0	36.0	36.0	32.0	36.0
48	34.21093519916422	36.0	36.0	36.0	32.0	36.0
49	34.21898764000707	36.0	36.0	36.0	32.0	36.0
50	33.64554844949825	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	9.0
22	53.0
23	174.0
24	428.0
25	1025.0
26	2162.0
27	4123.0
28	7136.0
29	10934.0
30	16087.0
31	23973.0
32	36228.0
33	60107.0
34	128285.0
35	354420.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.40623415842582	17.285240574893148	10.966593753439607	26.341931513241423
2	16.539403521825395	19.941251240079367	35.89657738095238	27.622767857142854
3	19.44065114245989	23.141846071336122	28.041844986305364	29.375657799898626
4	12.479539451657304	14.929845119849212	35.37613308036656	37.21448234812693
5	14.646607124588854	36.45531399094158	33.25200807259132	15.646070811878241
6	36.13576545938724	34.51503699960629	15.929981306441062	13.419216234565415
7	31.194179302049456	29.92531923006575	19.88557628815803	18.99492517972676
8	28.9805718395526	32.81195264327765	18.864257082892866	19.343218434276892
9	27.253985919466288	13.8204375443698	17.878433718879137	41.04714281728477
10	15.763408592783648	26.76913442848596	31.57905342356614	25.888403555164256
11	38.932892709557215	20.57193255480155	20.85791433256968	19.63726040307155
12	24.135932021588914	23.549088113388287	28.591202611501892	23.723777253520907
13	30.120468855111866	18.470547751981723	25.14438592194635	26.264597470960062
14	23.34215820914956	19.121563181047392	24.54421169781724	32.99206691198581
15	25.696819014610643	26.478037529489452	22.112979077604138	25.712164378295764
16	26.580029946709736	25.508799558549537	23.23520567437448	24.675964820366243
17	24.378047759731906	25.706274238699457	24.85189398988756	25.06378401168108
18	25.720069565648707	24.329996620919918	25.855387772690214	24.094546040741164
19	25.74688520117927	25.558710741444575	24.360222337269395	24.334181720106766
20	25.42463256379176	24.232499310233653	25.038518412886386	25.304349713088197
21	26.60638057121954	24.555836973336266	23.687971404922298	25.149811050521897
22	25.499499338134314	24.466089846329357	24.714870742436595	25.319540073099734
23	24.656279353820683	24.099351154622365	24.834378574772224	26.409990916784725
24	24.752381631444663	25.444783041358082	24.430904012425096	25.371931314772162
25	25.557780719403052	24.336971786231334	24.358362293186346	25.74688520117927
26	24.60326809745391	25.209952475873678	25.123925437032856	25.062853989639557
27	25.753085348122752	24.698750360383542	24.448419427540433	25.099744863953276
28	24.844453813555383	24.44748940549891	25.25676358529697	25.451293195648738
29	24.513830977794175	24.826938398440042	25.36991626701553	25.289314356750253
30	24.82910844987026	24.905215253601508	25.59110650922427	24.67456978730396
31	25.016662894910606	24.863674269080178	24.437724174062925	25.68193866194629
32	24.82461334333624	24.86258924336507	24.533671448013315	25.779125965285377
33	24.465314827961425	24.32457149234437	25.014337839806807	26.195775839887403
34	25.0586688904527	24.911260396871405	25.419672446236973	24.610398266438914
35	26.00326127729227	24.45182950835935	24.99496238060842	24.54994683373996
36	24.505305775746887	25.281719176744488	24.79376761229241	25.41920743521621
37	25.941414811531033	24.81562313026819	24.667439618318955	24.575522439881826
38	24.849568934783754	24.592417840302815	24.9676817340571	25.590331490856332
39	25.259708655095125	24.56529219742508	24.63054874400523	25.54445040347456
40	25.626912357822878	24.845848846617667	24.70789557712518	24.819343218434277
41	24.23001925145626	25.01929795736159	25.71200937462218	25.03867341655997
42	26.13067429698084	25.183291844016704	24.761836855533474	23.924197003468983
43	25.055413813307375	23.880795974864604	25.026738133693772	26.037052078134256
44	24.694720264870277	24.725410992240516	25.223747802822928	25.35612094006628
45	24.9796170169233	24.95373140343426	24.92257566504326	25.144075914599174
46	24.649459192182853	24.621558530937182	25.241263217938265	25.4877190589417
47	24.956366465885242	24.487170345937198	25.59048649452992	24.96597669364764
48	25.551580572459564	25.56041578185403	24.204908656335157	24.683094989351247
49	24.350612109506994	25.233513034258912	25.337210491888655	25.07866436434544
50	24.537236532505823	25.466173548313094	24.319921382136755	25.67666853704433
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	6.5
2	9.0
3	29.0
4	49.0
5	60.0
6	71.0
7	74.0
8	77.0
9	88.0
10	99.0
11	157.0
12	215.0
13	368.0
14	521.0
15	702.0
16	883.0
17	1047.0
18	1211.0
19	1436.5
20	1662.0
21	2150.0
22	2638.0
23	2884.5
24	3131.0
25	3722.5
26	4314.0
27	5489.5
28	6665.0
29	7463.5
30	8262.0
31	8693.5
32	9125.0
33	10277.0
34	11429.0
35	13263.5
36	15098.0
37	18198.0
38	21298.0
39	23337.5
40	25377.0
41	28017.5
42	30658.0
43	32096.5
44	33535.0
45	37928.5
46	42322.0
47	46227.0
48	50132.0
49	51507.5
50	52883.0
51	50031.0
52	47179.0
53	46206.0
54	45233.0
55	46342.5
56	47452.0
57	46100.0
58	44748.0
59	41055.5
60	37363.0
61	33077.5
62	28792.0
63	25110.0
64	21428.0
65	18511.5
66	15595.0
67	13383.5
68	11172.0
69	10243.0
70	9314.0
71	7222.0
72	5130.0
73	4651.0
74	4172.0
75	2993.5
76	1815.0
77	1632.0
78	1449.0
79	1263.5
80	1078.0
81	878.0
82	678.0
83	540.5
84	403.0
85	342.5
86	282.0
87	210.0
88	138.0
89	87.5
90	37.0
91	24.0
92	11.0
93	6.0
94	1.0
95	3.0
96	5.0
97	3.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013485319602074568
2	0.004030095513263664
3	4.65011020761192E-4
4	3.1000734717412804E-4
5	0.0
6	6.200146943482561E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	645146.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.16166619385853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.27962709468846	50.32359086920495
2	11.274076100639396	13.7908255822293
3	3.050855114462362	5.597861463497193
4	1.1228037859810265	2.746902014374885
5	0.5936707956396646	1.8154947515977788
6	0.34202945398973045	1.2551454776032553
7	0.22443044449616126	0.9608577951009457
8	0.1522533770365698	0.7449656178558695
9	0.11623192258267796	0.6398044245064941
>10	0.6794714995413499	7.995665619361117
>50	0.08545435688601216	3.71643570680464
>100	0.07528113183520632	8.844337690167329
>500	0.0035605940732766094	1.4111616927270065
>1k	2.543281480911864E-4	0.1569512949693066
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1009	0.15639870664934757	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	822	0.12741301968856664	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	807	0.12508796458476065	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	799	0.12384793519606414	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	720	0.11160264498268609	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	710	0.11005260824681545	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	663	0.10276743558822343	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.5500367358706402E-4	0.0
12	0.0	0.0	0.0	1.5500367358706402E-4	4.6501102076119207E-4
13	0.0	0.0	0.0	1.5500367358706402E-4	6.200146943482561E-4
14	0.0	0.0	0.0	1.5500367358706402E-4	6.200146943482561E-4
15	0.0	0.0	0.0	1.5500367358706402E-4	6.200146943482561E-4
16	0.0	0.0	0.0	1.5500367358706402E-4	6.200146943482561E-4
17	0.0	0.0	0.0	3.1000734717412804E-4	6.200146943482561E-4
18	0.0	0.0	0.0	4.6501102076119207E-4	6.200146943482561E-4
19	0.0	0.0	0.0	6.200146943482561E-4	6.200146943482561E-4
20	0.0	0.0	0.0	7.750183679353201E-4	6.200146943482561E-4
21	0.0	0.0	0.0	0.0012400293886965122	6.200146943482561E-4
22	0.0	0.0	0.0	0.0017050404094577042	6.200146943482561E-4
23	0.0	0.0	0.0	0.0035650844925024724	6.200146943482561E-4
24	0.0	0.0	0.0	0.005735135922721369	6.200146943482561E-4
25	0.0	0.0	0.0	0.008990213068049712	6.200146943482561E-4
26	0.0	0.0	0.0	0.011470271845442737	6.200146943482561E-4
27	0.0	0.0	0.0	0.01426033797000989	6.200146943482561E-4
28	0.0	0.0	0.0	0.021855517975776027	6.200146943482561E-4
29	0.0	0.0	0.0	0.0310007347174128	6.200146943482561E-4
30	0.0	0.0	0.0	0.04727612044405453	6.200146943482561E-4
31	0.0	0.0	0.0	0.07595180005766136	6.200146943482561E-4
32	0.0	0.0	0.0	0.1171827772318204	6.200146943482561E-4
33	0.0	0.0	0.0	0.15965378379467593	6.200146943482561E-4
34	0.0	0.0	0.0	0.21064999240482	6.200146943482561E-4
35	0.0	0.0	0.0	0.26753634061127246	6.200146943482561E-4
36	0.0	0.0	0.0	0.34271312230099854	6.200146943482561E-4
37	0.0	0.0	0.0	0.4605159142271672	6.200146943482561E-4
38	0.0	0.0	0.0	0.5983141800460671	6.200146943482561E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	160	0.0	24.749912	29
TCTAGCG	165	0.0	23.999914	28
CGCAATA	165	0.0	23.999914	36
TAGCGGC	170	0.0	23.294035	30
TACGAGC	85	2.3492066E-7	23.294035	14
ATACACT	190	0.0	23.157814	4
CGGTCCA	175	0.0	22.62849	10
GTACCGT	50	0.0025791982	21.999924	6
GCTATAC	50	0.0025791982	21.999924	3
TAGCACC	50	0.0025791982	21.999924	4
AATACGA	185	0.0	21.405329	39
ATACCGT	160	1.8189894E-12	20.624928	6
GATATAC	195	0.0	20.31077	1
CAATACG	195	0.0	20.307621	38
CGTCGTG	55	0.004481475	19.999931	29
ATACGAA	200	0.0	19.79993	40
CGAATGC	190	0.0	19.684143	43
CTAGACA	80	8.9805384E-5	19.249931	4
CTAGGAC	115	2.0178231E-7	19.130367	3
ACACGCT	265	0.0	19.094273	9
>>END_MODULE