FastQCFastQC Report
Thu 2 Feb 2017
SRR4062695_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062695_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390570
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG26900.19344585313936014No Hit
GTATCAACGCAGAGTACTTTTTTTT25350.18229934487296576No Hit
TCCATGTACTCTGCGTTGATACCAC22170.15943102468771797No Hit
GCGCAAGACGGACCAGAGCGAAAGC15210.10937960692377945No Hit
TATCAACGCAGAGTACTTTTTTTTT15110.10866047735820562No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15040.10815708666230395No Hit
GAATAACGCCGCCGCATCGCCAGTC14090.10132535578935256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1750.014.6498431
TATTAGG400.005293677614.2439282
GGGCGTA551.9568315E-413.82074716
CCGTCCG500.001498530613.3010339
CGTTATT2100.013.1134572
TCGAACG802.0045009E-613.0592833
TAGAGCG1207.403287E-1012.6653725
ACTATAC604.1000487E-412.66354753
GTATTAC905.4363227E-712.6603581
GTCTTAA905.4363227E-712.6603581
GTGTAGG1251.4206307E-912.1539441
CGCGTAA951.0385229E-612.00093210
CGGGCGT802.8625154E-511.8763515
GTTATTC2250.011.8193123
ACTAGAC658.0390746E-411.6894283
ATGCGTT907.452405E-611.61201312
CGAACGT907.4673517E-611.6099244
TAACACT907.4673517E-611.6099244
CGTATGA1501.7644197E-1011.40088710
CGGTTCT3000.011.40088712
Produced by FastQC (version 0.11.5)