##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062695_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1390570
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22889103029693	32.0	32.0	32.0	32.0	32.0
2	30.949586860064578	32.0	32.0	32.0	32.0	32.0
3	30.9341075961656	32.0	32.0	32.0	32.0	32.0
4	30.997972773754647	32.0	32.0	32.0	32.0	32.0
5	30.83121885270069	32.0	32.0	32.0	32.0	32.0
6	34.47196401475654	36.0	36.0	36.0	32.0	36.0
7	34.37727406746873	36.0	36.0	36.0	32.0	36.0
8	34.389850924441056	36.0	36.0	36.0	32.0	36.0
9	34.50838433160502	36.0	36.0	36.0	32.0	36.0
10	34.22896438151262	36.0	36.0	36.0	32.0	36.0
11	34.477276224857434	36.0	36.0	36.0	32.0	36.0
12	34.29324881163839	36.0	36.0	36.0	32.0	36.0
13	34.408049217227465	36.0	36.0	36.0	32.0	36.0
14	34.319018100491164	36.0	36.0	36.0	32.0	36.0
15	34.26651157439036	36.0	36.0	36.0	32.0	36.0
16	34.26970450966151	36.0	36.0	36.0	32.0	36.0
17	34.196881854203674	36.0	36.0	36.0	32.0	36.0
18	34.20636213926663	36.0	36.0	36.0	32.0	36.0
19	34.1913790747679	36.0	36.0	36.0	32.0	36.0
20	34.18974449326535	36.0	36.0	36.0	32.0	36.0
21	34.15469124172102	36.0	36.0	36.0	32.0	36.0
22	34.11902744917552	36.0	36.0	36.0	32.0	36.0
23	34.083012721402014	36.0	36.0	36.0	32.0	36.0
24	34.051556555944686	36.0	36.0	36.0	32.0	36.0
25	33.59867536334021	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	10.0
4	50.0
5	117.0
6	225.0
7	60.0
8	217.0
9	264.0
10	173.0
11	38.0
12	110.0
13	69.0
14	212.0
15	273.0
16	436.0
17	640.0
18	784.0
19	1104.0
20	1723.0
21	2435.0
22	3542.0
23	5004.0
24	7190.0
25	10110.0
26	14218.0
27	18731.0
28	25482.0
29	34476.0
30	45432.0
31	63124.0
32	92483.0
33	135033.0
34	313292.0
35	613511.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.156944196723956	17.291521630314083	10.625507027910269	25.926027145051695
2	17.62919004206313	19.874378743629222	35.92251581951905	26.5739153947886
3	19.571922844853315	23.6892995813727	27.866726789054564	28.872050784719423
4	12.91856393112957	14.954987093447183	35.82059280239417	36.305856173029085
5	14.891223183468169	36.101155749179554	33.3126392891416	15.694981778210682
6	34.087849772040684	35.38315655124929	16.661282745094937	13.867710931615083
7	30.382873077269046	29.98370063866151	20.66787802464694	18.96554825942251
8	28.66379620592461	31.94200920735552	19.500202976411547	19.893991610308323
9	27.673100843046022	13.683374026811629	18.43371377896531	40.20981135117704
10	16.40044178019563	26.198083987195865	31.10820878866117	26.293265443947337
11	38.17205655876803	21.002471692176766	21.627486541769294	19.19798520728591
12	24.536400641438757	23.6002370471927	28.597165946716224	23.266196364652316
13	29.495897847584967	19.371087295387373	25.211227549760423	25.921787307267234
14	23.62601520649732	19.711710154944992	24.408516214503774	32.25375842405391
15	25.95069715271413	26.47135687581128	21.967635872245424	25.61031009922916
16	26.262530272793505	25.685007444011532	23.38305126720978	24.669411015985187
17	24.17363568617992	25.308214863001616	24.904941733836854	25.613207716981606
18	25.169575248206318	24.596979760420055	25.979360064056962	24.25408492731666
19	26.061137597397405	24.71721358987708	24.647367560767847	24.574281251957668
20	25.77893647766275	24.093013745281382	24.85749647386121	25.270553303194653
21	26.933694695777273	23.72225878408834	24.1260553938673	25.217991126267087
22	25.879102755599853	24.335082440414887	24.628560810878938	25.15725399310632
23	24.749115712096597	23.9739955987234	25.19723652829145	26.07965216088855
24	24.88737992712819	25.319354234773968	24.568745769546496	25.22452006855135
25	25.179005518496755	24.390329957718407	24.525340153688408	25.905324370096427
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	108.0
1	108.0
2	372.5
3	637.0
4	637.0
5	637.0
6	1569.0
7	2501.0
8	2501.0
9	2501.0
10	2673.5
11	2846.0
12	2846.0
13	2846.0
14	3114.5
15	3383.0
16	3383.0
17	3383.0
18	5357.0
19	7331.0
20	7331.0
21	7331.0
22	12029.5
23	16728.0
24	16728.0
25	16728.0
26	25727.5
27	34727.0
28	34727.0
29	34727.0
30	44134.0
31	53541.0
32	53541.0
33	53541.0
34	66919.5
35	80298.0
36	80298.0
37	80298.0
38	93452.0
39	106606.0
40	106606.0
41	106606.0
42	125711.5
43	144817.0
44	144817.0
45	144817.0
46	166853.0
47	188889.0
48	188889.0
49	188889.0
50	190211.5
51	191534.0
52	191534.0
53	191534.0
54	177649.0
55	163764.0
56	163764.0
57	163764.0
58	150279.0
59	136794.0
60	136794.0
61	136794.0
62	121497.5
63	106201.0
64	106201.0
65	106201.0
66	87930.0
67	69659.0
68	69659.0
69	69659.0
70	53268.0
71	36877.0
72	36877.0
73	36877.0
74	28919.0
75	20961.0
76	20961.0
77	20961.0
78	17210.5
79	13460.0
80	13460.0
81	13460.0
82	9268.5
83	5077.0
84	5077.0
85	5077.0
86	3711.0
87	2345.0
88	2345.0
89	2345.0
90	1531.5
91	718.0
92	718.0
93	718.0
94	435.0
95	152.0
96	152.0
97	152.0
98	384.0
99	616.0
100	616.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008198077047541654
2	0.0029484312188527008
3	0.005249645828688955
4	0.01373537470246014
5	0.034230567321314284
6	0.04523324967459387
7	0.06795774394672688
8	0.08960354387049915
9	0.09506892856886026
10	0.11822490058033756
11	0.11815298762378017
12	0.1306658420647648
13	0.11973507266804259
14	0.12153289658197718
15	0.11239995109918954
16	0.11031447535902543
17	0.12117333179919025
18	0.1298747995426336
19	0.1295152347598467
20	0.11915976901558355
21	0.12599149988853492
22	0.1475653868557498
23	0.1353401842409947
24	0.1316007105000108
25	0.12843654041148594
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1390570.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.02573935239097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.33040685128852	38.89231849010402
2	12.339036414287683	12.09860766213058
3	3.5695866688887237	5.250048768741244
4	1.420471206547934	2.7855860451918133
5	0.7648069703513927	1.8747613591669592
6	0.4816051428020547	1.4166628921070739
7	0.34109118135460575	1.1705573146743016
8	0.2259216099464303	0.8860779170644986
9	0.17447330640175865	0.769831455924222
>10	1.058564101451414	10.108508114252885
>50	0.14119739323913572	4.90011669646199
>100	0.13843701632031316	13.894635440316083
>500	0.010581161965732033	3.3934128809315984
>1k	0.0038209751542921232	2.5588749629326877
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	2690	0.19344585313936014	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2535	0.18229934487296576	No Hit
TCCATGTACTCTGCGTTGATACCAC	2217	0.15943102468771797	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1521	0.10937960692377945	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1511	0.10866047735820562	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1504	0.10815708666230395	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1409	0.10132535578935256	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.191295655738294E-5	0.0
3	0.0	0.0	0.0	7.191295655738294E-5	0.0
4	0.0	0.0	0.0	7.191295655738294E-5	0.0
5	0.0	0.0	0.0	7.191295655738294E-5	0.0
6	0.0	0.0	0.0	7.191295655738294E-5	1.438259131147659E-4
7	0.0	0.0	0.0	1.438259131147659E-4	1.438259131147659E-4
8	0.0	0.0	0.0	1.438259131147659E-4	1.438259131147659E-4
9	0.0	0.0	0.0	1.438259131147659E-4	1.438259131147659E-4
10	0.0	0.0	0.0	1.438259131147659E-4	1.438259131147659E-4
11	0.0	0.0	0.0	1.438259131147659E-4	1.438259131147659E-4
12	0.0	0.0	0.0	1.438259131147659E-4	5.033906959016806E-4
13	0.0	0.0	0.0	1.438259131147659E-4	5.753036524590635E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	175	0.0	14.649843	1
TATTAGG	40	0.0052936776	14.243928	2
GGGCGTA	55	1.9568315E-4	13.820747	16
CCGTCCG	50	0.0014985306	13.301033	9
CGTTATT	210	0.0	13.113457	2
TCGAACG	80	2.0045009E-6	13.059283	3
TAGAGCG	120	7.403287E-10	12.665372	5
ACTATAC	60	4.1000487E-4	12.6635475	3
GTATTAC	90	5.4363227E-7	12.660358	1
GTCTTAA	90	5.4363227E-7	12.660358	1
GTGTAGG	125	1.4206307E-9	12.153944	1
CGCGTAA	95	1.0385229E-6	12.000932	10
CGGGCGT	80	2.8625154E-5	11.87635	15
GTTATTC	225	0.0	11.819312	3
ACTAGAC	65	8.0390746E-4	11.689428	3
ATGCGTT	90	7.452405E-6	11.612013	12
CGAACGT	90	7.4673517E-6	11.609924	4
TAACACT	90	7.4673517E-6	11.609924	4
CGTATGA	150	1.7644197E-10	11.400887	10
CGGTTCT	300	0.0	11.400887	12
>>END_MODULE