FastQCFastQC Report
Thu 2 Feb 2017
SRR4062695_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062695_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390570
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15260.10973917170656637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATA2150.020.4671572
ATACCGT4000.020.3498346
ACCGTCG4150.020.1444158
CCGTCGT4100.019.3169169
GTCGTAG4250.019.15278611
CGCAATA4250.019.15278636
TACCGTC4250.018.6351437
CGATTTA2150.018.41845518
TAGGACG3250.018.2767734
TGTAGGA5600.018.073232
CAATACG4750.018.0630138
GTATTAA1102.7905407E-618.0043851
GTATCAA31250.017.8155381
CTAGCGG4700.017.78708829
AATACGA5000.017.1598639
CGTCGTA4650.017.03211810
CGATGTA1954.9112714E-1116.92293736
CTTATAG1053.7752667E-516.7617683
TCTATAC1053.7752667E-516.7617683
TCTAGCG5000.016.71986428
Produced by FastQC (version 0.11.5)