##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062695_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1390570
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2958556563136	32.0	32.0	32.0	32.0	32.0
2	31.403026097211935	32.0	32.0	32.0	32.0	32.0
3	31.492939585925196	32.0	32.0	32.0	32.0	32.0
4	31.59700554448895	32.0	32.0	32.0	32.0	32.0
5	31.52235773819369	32.0	32.0	32.0	32.0	32.0
6	35.12630288299043	36.0	36.0	36.0	36.0	36.0
7	35.15396779737805	36.0	36.0	36.0	36.0	36.0
8	35.081265955687236	36.0	36.0	36.0	36.0	36.0
9	35.2197674334985	36.0	36.0	36.0	36.0	36.0
10	35.08991996087935	36.0	36.0	36.0	36.0	36.0
11	35.21867219917012	36.0	36.0	36.0	36.0	36.0
12	35.13191712750886	36.0	36.0	36.0	36.0	36.0
13	35.17253212711334	36.0	36.0	36.0	36.0	36.0
14	35.13633330217105	36.0	36.0	36.0	36.0	36.0
15	35.09611741947547	36.0	36.0	36.0	36.0	36.0
16	35.110660376680066	36.0	36.0	36.0	36.0	36.0
17	35.0802347238902	36.0	36.0	36.0	36.0	36.0
18	35.09214207123698	36.0	36.0	36.0	36.0	36.0
19	35.08625815313145	36.0	36.0	36.0	36.0	36.0
20	35.07141316150931	36.0	36.0	36.0	36.0	36.0
21	35.059972529250594	36.0	36.0	36.0	36.0	36.0
22	35.0445500765873	36.0	36.0	36.0	36.0	36.0
23	35.01050648295303	36.0	36.0	36.0	36.0	36.0
24	34.98799772755057	36.0	36.0	36.0	36.0	36.0
25	34.954307226533004	36.0	36.0	36.0	32.0	36.0
26	34.91492265761522	36.0	36.0	36.0	32.0	36.0
27	34.893691795450785	36.0	36.0	36.0	32.0	36.0
28	34.88766405143215	36.0	36.0	36.0	32.0	36.0
29	34.864880588535634	36.0	36.0	36.0	32.0	36.0
30	34.8386719115183	36.0	36.0	36.0	32.0	36.0
31	34.838495005645164	36.0	36.0	36.0	32.0	36.0
32	34.79828128033828	36.0	36.0	36.0	32.0	36.0
33	34.78612295677312	36.0	36.0	36.0	32.0	36.0
34	34.7752547516486	36.0	36.0	36.0	32.0	36.0
35	34.749226576152225	36.0	36.0	36.0	32.0	36.0
36	34.723359485678536	36.0	36.0	36.0	32.0	36.0
37	34.70334179509122	36.0	36.0	36.0	32.0	36.0
38	34.668575476243554	36.0	36.0	36.0	32.0	36.0
39	34.6465931236831	36.0	36.0	36.0	32.0	36.0
40	34.632002703927164	36.0	36.0	36.0	32.0	36.0
41	34.605545927209704	36.0	36.0	36.0	32.0	36.0
42	34.56454835067634	36.0	36.0	36.0	32.0	36.0
43	34.56303386381124	36.0	36.0	36.0	32.0	36.0
44	34.540087877632914	36.0	36.0	36.0	32.0	36.0
45	34.49380469879258	36.0	36.0	36.0	32.0	36.0
46	34.50595655019165	36.0	36.0	36.0	32.0	36.0
47	34.47494624506497	36.0	36.0	36.0	32.0	36.0
48	34.44278461350382	36.0	36.0	36.0	32.0	36.0
49	34.431176424056325	36.0	36.0	36.0	32.0	36.0
50	33.914883824618684	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	8.0
21	26.0
22	87.0
23	280.0
24	809.0
25	1882.0
26	3962.0
27	7574.0
28	13091.0
29	20609.0
30	30588.0
31	44886.0
32	67290.0
33	111081.0
34	248031.0
35	840364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.72793678170427	17.83254228949831	11.026088982901513	26.41343194589591
2	17.033487042145747	20.347599582310924	35.6469721857048	26.97194118983853
3	18.984828447182903	23.963747057846355	28.21439949142901	28.837025003541733
4	12.70387352506314	15.384720488318443	36.044767318103105	35.86663866851531
5	14.666863228747923	36.322659053481665	33.25219154735108	15.758286170419325
6	34.247328955723624	35.57708004179608	16.42250861343012	13.753082389050174
7	30.507130169642664	30.02941239923197	20.59241893611972	18.871038495005646
8	28.717884549418258	32.080680642240694	19.364375302484092	19.837059505856956
9	27.840238175712116	13.503167765736352	18.223462321206412	40.43313173734512
10	16.38054898351036	26.224569780737394	31.023752849550906	26.37112838620134
11	38.49378312490561	20.892583616790237	21.493416368827173	19.120216889476975
12	24.563955787914306	23.497630468081436	28.552823662239224	23.38559008176503
13	29.673155612446696	19.310498572527813	25.109487476358616	25.906858338666876
14	23.657564883465053	19.631374184686855	24.490173094486433	32.22088783736166
15	26.104259404416897	26.530487497932505	21.85233393500507	25.512919162645538
16	26.458718367288235	25.55829623823324	23.30792408868306	24.675061305795467
17	24.391220866263474	25.364418907354537	24.74805295670121	25.49630726968078
18	25.46106992096766	24.578266466269227	25.769288852772604	24.19137475999051
19	26.282028233026743	24.71396621529301	24.48039293239463	24.52361261928562
20	25.901249128055404	24.004616811810983	24.760206246359406	25.33392781377421
21	27.156561697721077	23.68194337573801	23.963842165442948	25.197652761097967
22	25.939938298683273	24.361808467031505	24.50369273031922	25.194560503966002
23	24.880300883810236	23.913934573592126	25.027938183622545	26.177826358975096
24	25.08676298208648	25.165579582473374	24.480249106481512	25.26740832895863
25	25.437698210086513	24.179149557375755	24.460760695254464	25.922391537283275
26	24.8774962785045	24.86778802936925	25.22620220485125	25.028513487275
27	26.012714210719345	24.56036014008644	24.342607707630684	25.084317941563533
28	24.83485189526597	24.288960642038877	25.333496336034862	25.542691126660287
29	25.043111817456147	24.45342557368561	24.83844754309384	25.665015065764397
30	24.767109890188916	24.932509690270894	25.36902133657421	24.93135908296598
31	25.449420022005363	24.238909224274938	24.346347181371666	25.96532357234803
32	25.074106301732385	24.59027593001431	24.319307909706094	26.016309858547217
33	24.884184183464335	23.981748491625737	24.793933422984818	26.34013390192511
34	25.281790920270105	24.307586097787237	25.51299107560209	24.897631906340564
35	26.028319322292297	24.004688724767544	25.01427472187664	24.95271723106352
36	25.101720877050422	24.66391479752907	24.504052295102007	25.7303120303185
37	25.983445637400493	24.53037243720201	24.175769648417557	25.310412276979942
38	25.059579884507794	24.14074803857411	25.016719762399592	25.78295231451851
39	25.718374479529977	23.752633812033913	24.630259533860215	25.898732174575894
40	25.89305105100786	24.265373192288052	24.880300883810236	24.96127487289385
41	24.7625793739258	24.504411859884794	25.64674917479882	25.08625959139058
42	26.08218212675378	25.13983474402583	24.68534485858317	24.09263827063722
43	25.186434339875014	23.567889426637997	25.05418641276599	26.191489820721
44	24.514479673802832	24.21165421373969	25.365713340572572	25.90815277188491
45	25.062887880509432	24.360801685639704	25.06734648381599	25.50896395003488
46	24.941678508350545	23.849103387968082	25.387539480269933	25.821678623411444
47	24.7625793739258	24.02561539512574	25.467901651840613	25.743903579107847
48	25.264963288435677	25.083167334258615	24.621486153160216	25.030383224145492
49	24.42933473323889	24.758767987228257	25.265610505044695	25.54628677448816
50	24.20016554374504	25.251965202733555	24.854358179996822	25.693511073524576
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	10.0
2	13.0
3	51.0
4	89.0
5	107.5
6	126.0
7	137.5
8	149.0
9	189.5
10	230.0
11	335.0
12	440.0
13	781.0
14	1122.0
15	1633.5
16	2145.0
17	2540.5
18	2936.0
19	3238.0
20	3540.0
21	4195.5
22	4851.0
23	5247.0
24	5643.0
25	6480.5
26	7318.0
27	9389.5
28	11461.0
29	13373.5
30	15286.0
31	16678.5
32	18071.0
33	21423.5
34	24776.0
35	28857.5
36	32939.0
37	40302.0
38	47665.0
39	51759.5
40	55854.0
41	61937.5
42	68021.0
43	70520.5
44	73020.0
45	81737.5
46	90455.0
47	97588.0
48	104721.0
49	108607.5
50	112494.0
51	110087.5
52	107681.0
53	104207.0
54	100733.0
55	102335.5
56	103938.0
57	99759.5
58	95581.0
59	88352.0
60	81123.0
61	71685.5
62	62248.0
63	54454.5
64	46661.0
65	39622.5
66	32584.0
67	27573.5
68	22563.0
69	20562.0
70	18561.0
71	14865.5
72	11170.0
73	10570.5
74	9971.0
75	7432.0
76	4893.0
77	4276.0
78	3659.0
79	3037.0
80	2415.0
81	1913.0
82	1411.0
83	1200.0
84	989.0
85	803.5
86	618.0
87	449.5
88	281.0
89	185.5
90	90.0
91	54.0
92	18.0
93	11.5
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015317459746722566
2	0.004458603306557743
3	5.033906959016806E-4
4	1.438259131147659E-4
5	0.0
6	6.472166090164465E-4
7	0.0
8	7.191295655738294E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.438259131147659E-4
47	0.0
48	0.0
49	0.0
50	7.191295655738294E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1390570.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.07175550149211
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.14645759406156	42.5351320174274
2	12.180050123151846	12.928332842636669
3	3.330368618989204	5.302455272305108
4	1.3317983218154315	2.827234996507443
5	0.711496901717385	1.888019480400711
6	0.44805561879605593	1.426745895108842
7	0.29496388125693224	1.095797569148734
8	0.21776434363856684	0.9245708802023143
9	0.15503102623610907	0.740499184759337
>10	0.9397549542762679	9.607688750558108
>50	0.1207146628989228	4.442553660404417
>100	0.1131918898979281	12.077825977153983
>500	0.008308892882778215	2.8877590136627704
>1k	0.0020431703810110365	1.3153844597243292
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1526	0.10973917170656637	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1573886967214884E-4
2	0.0	0.0	0.0	0.0	3.595647827869147E-4
3	0.0	0.0	0.0	0.0	3.595647827869147E-4
4	0.0	0.0	0.0	0.0	3.595647827869147E-4
5	0.0	0.0	0.0	0.0	3.595647827869147E-4
6	0.0	0.0	0.0	0.0	5.753036524590635E-4
7	0.0	0.0	0.0	0.0	5.753036524590635E-4
8	0.0	0.0	0.0	0.0	5.753036524590635E-4
9	0.0	0.0	0.0	0.0	5.753036524590635E-4
10	0.0	0.0	0.0	0.0	7.191295655738294E-4
11	0.0	0.0	0.0	0.0	7.191295655738294E-4
12	0.0	0.0	0.0	0.0	0.0010067813918033612
13	0.0	0.0	0.0	0.0	0.001294433218032893
14	0.0	0.0	0.0	0.0	0.0013663461745902759
15	0.0	0.0	0.0	0.0	0.0015101720877050417
16	0.0	0.0	0.0	0.0	0.0015101720877050417
17	0.0	0.0	0.0	7.191295655738294E-5	0.0015820850442624247
18	0.0	0.0	0.0	7.191295655738294E-5	0.0016539980008198076
19	0.0	0.0	0.0	1.438259131147659E-4	0.0016539980008198076
20	0.0	0.0	0.0	2.1573886967214884E-4	0.0016539980008198076
21	0.0	0.0	0.0	2.876518262295318E-4	0.0016539980008198076
22	0.0	0.0	0.0	4.314777393442977E-4	0.0017259109573771907
23	0.0	0.0	0.0	0.00122252026147551	0.0017259109573771907
24	0.0	0.0	0.0	0.0021573886967214883	0.0017259109573771907
25	0.0	0.0	0.0	0.002804605305737935	0.0017259109573771907
26	0.0	0.0	0.0	0.003811386697541296	0.0018697368704919566
27	0.0	0.0	0.0	0.00489008104590204	0.0018697368704919566
28	0.0	0.0	0.0	0.011290334179509122	0.0018697368704919566
29	0.0	0.0	0.0	0.025529099577870945	0.0019416498270493395
30	0.0	0.0	0.0	0.0560921061147587	0.0019416498270493395
31	0.0	0.0	0.0	0.11700238031886205	0.0019416498270493395
32	0.0	0.0	0.0	0.18841194618034332	0.0019416498270493395
33	0.0	0.0	0.0	0.26197890073854607	0.0019416498270493395
34	7.191295655738294E-5	0.0	0.0	0.34503836556232337	0.0021573886967214883
35	7.191295655738294E-5	0.0	0.0	0.4334913021279044	0.0021573886967214883
36	7.191295655738294E-5	0.0	0.0	0.5660987940197185	0.0021573886967214883
37	7.191295655738294E-5	0.0	0.0	0.7587536046369474	0.0021573886967214883
38	7.191295655738294E-5	0.0	0.0	1.0158424243295914	0.0023731275663936373
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTATA	215	0.0	20.467157	2
ATACCGT	400	0.0	20.349834	6
ACCGTCG	415	0.0	20.144415	8
CCGTCGT	410	0.0	19.316916	9
GTCGTAG	425	0.0	19.152786	11
CGCAATA	425	0.0	19.152786	36
TACCGTC	425	0.0	18.635143	7
CGATTTA	215	0.0	18.418455	18
TAGGACG	325	0.0	18.276773	4
TGTAGGA	560	0.0	18.07323	2
CAATACG	475	0.0	18.06301	38
GTATTAA	110	2.7905407E-6	18.004385	1
GTATCAA	3125	0.0	17.815538	1
CTAGCGG	470	0.0	17.787088	29
AATACGA	500	0.0	17.15986	39
CGTCGTA	465	0.0	17.032118	10
CGATGTA	195	4.9112714E-11	16.922937	36
CTTATAG	105	3.7752667E-5	16.761768	3
TCTATAC	105	3.7752667E-5	16.761768	3
TCTAGCG	500	0.0	16.719864	28
>>END_MODULE