FastQCFastQC Report
Thu 2 Feb 2017
SRR4062694_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062694_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619828
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30590.1888472109384453No Hit
GCGCAAGACGGACCAGAGCGAAAGC21440.13235973202093063No Hit
GTCTTGCGCCGGTCCAAGAATTTCA20170.12451939341707886No Hit
TATCAACGCAGAGTACTTTTTTTTT19540.12063009158997129No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17690.10920912590719509No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17670.10908565600792183No Hit
GAACTACGACGGTATCTGATCGTCT17380.1072953424684596No Hit
GAATAACGCCGCCGCATCGCCAGTC17220.10630758327427356No Hit
GAATAGGACCGCGGTTCTATTTTGT17210.10624584832463695No Hit
GGGTAGGCACACGCTGAGCCAGTCA16830.10389992023844506No Hit
GATTAAGAGGGACGGCCGGGGGCAT16640.10272695619534912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG508.724816E-515.1978485
ACGCTTA704.4247827E-714.93199717
GCGTAAC1500.014.56775911
AATCGTA602.5658266E-514.25150713
CAAGACG4150.013.7317224
CGCGTAA1600.013.65769510
TCGCGTA1650.013.2425979
GCGTTAT1800.013.1868691
AAGACGG4250.012.9628715
GACGCTT751.4751853E-512.66957416
CGACGCT751.4765932E-512.66839915
TTTCGGT751.4765932E-512.66839915
CGCGCCA1051.990702E-812.66800810
ATATACG2000.012.3501626
CCGACCA2700.012.3149759
GTAAACG1650.012.09368417
CGTAACT2000.011.87662412
CGACCAT2800.011.87625610
GGTTCTA2900.011.79435113
TACGCTA2100.011.7620589
Produced by FastQC (version 0.11.5)