##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062694_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1619828
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.161961023022197	32.0	32.0	32.0	32.0	32.0
2	30.813775907071616	32.0	32.0	32.0	32.0	32.0
3	30.800046671621924	32.0	32.0	32.0	32.0	32.0
4	30.883583936072224	32.0	32.0	32.0	32.0	32.0
5	30.674746948441438	32.0	32.0	32.0	32.0	32.0
6	34.313889499378945	36.0	36.0	36.0	32.0	36.0
7	34.18956580575222	36.0	36.0	36.0	32.0	36.0
8	34.19462621957393	36.0	36.0	36.0	32.0	36.0
9	34.35815345826841	36.0	36.0	36.0	32.0	36.0
10	34.018398249690705	36.0	36.0	36.0	32.0	36.0
11	34.33492691816662	36.0	36.0	36.0	32.0	36.0
12	34.114626985087305	36.0	36.0	36.0	32.0	36.0
13	34.24136760199231	36.0	36.0	36.0	32.0	36.0
14	34.145289499872824	36.0	36.0	36.0	32.0	36.0
15	34.07548579231869	36.0	36.0	36.0	32.0	36.0
16	34.089737305442306	36.0	36.0	36.0	32.0	36.0
17	34.00041485886156	36.0	36.0	36.0	32.0	36.0
18	34.01528804292801	36.0	36.0	36.0	32.0	36.0
19	34.00555799751579	36.0	36.0	36.0	32.0	36.0
20	33.999246833614436	36.0	36.0	36.0	32.0	36.0
21	33.97766614726996	36.0	36.0	36.0	32.0	36.0
22	33.92874799052739	36.0	36.0	36.0	32.0	36.0
23	33.89193297066108	36.0	36.0	36.0	32.0	36.0
24	33.870377595645955	36.0	36.0	36.0	32.0	36.0
25	33.40130680541391	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	70.0
5	157.0
6	284.0
7	85.0
8	221.0
9	287.0
10	175.0
11	51.0
12	116.0
13	99.0
14	262.0
15	374.0
16	516.0
17	755.0
18	1011.0
19	1384.0
20	2200.0
21	3153.0
22	4937.0
23	7098.0
24	10060.0
25	14220.0
26	19323.0
27	25364.0
28	34325.0
29	45487.0
30	58984.0
31	79994.0
32	114535.0
33	164191.0
34	364324.0
35	665778.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.80948641296631	17.29850972954351	10.807208221739007	26.084795635751178
2	17.388982489456815	19.990097341160798	36.02950300876608	26.59141716061631
3	19.55600226703331	23.521211455185963	27.946680265573814	28.976106012206916
4	12.886859556181232	15.0045073414095	35.42017066152953	36.68846244087973
5	14.878348233585225	36.2501636598543	33.28162093660373	15.589867169956745
6	34.59078087294563	34.85309567912482	16.751768169793714	13.80435527813584
7	30.52819318134783	30.233328205783998	20.601257670474027	18.637220942394144
8	28.45187508920831	32.20835601515815	19.64745492933466	19.692313966298876
9	27.36818038571102	14.194252028350562	18.671700724830224	39.765866861108194
10	16.072683490335425	26.332344202645498	31.34613043050981	26.24884187650927
11	37.67631510470588	21.32759919723743	21.833312421619706	19.16277327643699
12	24.635525711470503	23.956925119657598	28.2689256191195	23.138623549752403
13	29.273000572333423	19.217547273134077	25.323652698681904	26.185799455850596
14	23.4126299796829	19.74356074021741	24.82549360292586	32.018315677173824
15	25.38428496821703	26.973217017252725	22.149746137990924	25.492751876539316
16	25.99331281442751	25.71031260429414	23.69433010716802	24.602044474110333
17	24.241723737236384	25.79233317674982	25.055751971716074	24.91019111429772
18	25.066775460752222	24.59685918892773	26.11979094227423	24.21657440804582
19	25.409406394726364	25.188847431056775	25.30703934208151	24.094706832135344
20	25.487348019548932	24.377135885094713	25.027613142805222	25.107902952551132
21	26.587013121879938	24.30230516839375	24.48181307935189	24.62886863037442
22	25.682861110458482	24.53619605956772	24.819554515743306	24.961388314230494
23	24.193529441947906	24.32038615876996	25.56243211280109	25.92365228648104
24	24.410314739890644	25.32143155187815	25.14741745148159	25.120836256749612
25	24.680865531128397	24.74274216728945	25.194608511282752	25.3817837902994
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	215.0
1	215.0
2	528.0
3	841.0
4	841.0
5	841.0
6	2184.0
7	3527.0
8	3527.0
9	3527.0
10	3745.0
11	3963.0
12	3963.0
13	3963.0
14	4537.5
15	5112.0
16	5112.0
17	5112.0
18	8244.0
19	11376.0
20	11376.0
21	11376.0
22	17711.5
23	24047.0
24	24047.0
25	24047.0
26	35082.5
27	46118.0
28	46118.0
29	46118.0
30	56497.0
31	66876.0
32	66876.0
33	66876.0
34	83027.0
35	99178.0
36	99178.0
37	99178.0
38	113114.0
39	127050.0
40	127050.0
41	127050.0
42	145590.5
43	164131.0
44	164131.0
45	164131.0
46	188177.5
47	212224.0
48	212224.0
49	212224.0
50	213540.0
51	214856.0
52	214856.0
53	214856.0
54	199649.5
55	184443.0
56	184443.0
57	184443.0
58	170507.5
59	156572.0
60	156572.0
61	156572.0
62	139074.0
63	121576.0
64	121576.0
65	121576.0
66	102133.0
67	82690.0
68	82690.0
69	82690.0
70	62870.5
71	43051.0
72	43051.0
73	43051.0
74	34026.5
75	25002.0
76	25002.0
77	25002.0
78	20306.0
79	15610.0
80	15610.0
81	15610.0
82	11008.0
83	6406.0
84	6406.0
85	6406.0
86	4769.0
87	3132.0
88	3132.0
89	3132.0
90	2020.0
91	908.0
92	908.0
93	908.0
94	553.0
95	198.0
96	198.0
97	198.0
98	462.0
99	726.0
100	726.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007531663855668627
2	0.0037658319278343136
3	0.005556145467296528
4	0.015310267509883766
5	0.0380287289761629
6	0.04821499566620653
7	0.06920487854266008
8	0.08797230323219503
9	0.09371365354840143
10	0.11785201885632302
11	0.11822242855414279
12	0.1288408398916428
13	0.11667905481322709
14	0.12217346533088698
15	0.112542813187573
16	0.11161678894302358
17	0.1202596818921515
18	0.1292112495894626
19	0.13087809322965155
20	0.11970406734542186
21	0.1279148156470934
22	0.1507567470126458
23	0.13921231143059634
24	0.132297997071294
25	0.1292112495894626
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1619828.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.181583751729086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.83552704090556	42.45779767689351
2	12.678586992446823	13.485346719847874
3	3.450050805647887	5.504374976048505
4	1.3516156108661836	2.875242352376977
5	0.7024501828692735	1.867870661583984
6	0.3940605465517891	1.2574058375817665
7	0.2733814491410284	1.0177200903563868
8	0.19014227561243635	0.8089653884181711
9	0.13996373413452193	0.6699143744171839
>10	0.7779722281058977	7.826797779844279
>50	0.09201053096445751	3.4228649667873325
>100	0.09680666058976994	10.825952220150228
>500	0.012899637201655812	4.5310283049304125
>1k	0.004532304962743934	3.4487186507634653
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3059	0.1888472109384453	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2144	0.13235973202093063	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2017	0.12451939341707886	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1954	0.12063009158997129	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1769	0.10920912590719509	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1767	0.10908565600792183	No Hit
GAACTACGACGGTATCTGATCGTCT	1738	0.1072953424684596	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1722	0.10630758327427356	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1721	0.10624584832463695	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1683	0.10389992023844506	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1664	0.10272695619534912	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	6.173494963662808E-5	0.0
6	0.0	0.0	0.0	6.173494963662808E-5	0.0
7	0.0	0.0	0.0	6.173494963662808E-5	0.0
8	0.0	0.0	0.0	1.2346989927325617E-4	0.0
9	0.0	0.0	0.0	1.2346989927325617E-4	0.0
10	0.0	0.0	0.0	1.2346989927325617E-4	0.0
11	0.0	0.0	0.0	1.2346989927325617E-4	0.0
12	0.0	0.0	0.0	2.4693979854651234E-4	0.0
13	0.0	0.0	0.0	2.4693979854651234E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	50	8.724816E-5	15.197848	5
ACGCTTA	70	4.4247827E-7	14.931997	17
GCGTAAC	150	0.0	14.567759	11
AATCGTA	60	2.5658266E-5	14.251507	13
CAAGACG	415	0.0	13.731722	4
CGCGTAA	160	0.0	13.657695	10
TCGCGTA	165	0.0	13.242597	9
GCGTTAT	180	0.0	13.186869	1
AAGACGG	425	0.0	12.962871	5
GACGCTT	75	1.4751853E-5	12.669574	16
CGACGCT	75	1.4765932E-5	12.668399	15
TTTCGGT	75	1.4765932E-5	12.668399	15
CGCGCCA	105	1.990702E-8	12.668008	10
ATATACG	200	0.0	12.350162	6
CCGACCA	270	0.0	12.314975	9
GTAAACG	165	0.0	12.093684	17
CGTAACT	200	0.0	11.876624	12
CGACCAT	280	0.0	11.876256	10
GGTTCTA	290	0.0	11.794351	13
TACGCTA	210	0.0	11.762058	9
>>END_MODULE