FastQCFastQC Report
Thu 2 Feb 2017
SRR4062694_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062694_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619828
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20340.1255688875609015No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC20100.12408724876962246No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17700.10927086085683173No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG17340.1070484026699131No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16660.1028504260946224No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16390.10118358245443343No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA16360.10099837760552356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG1850.021.4052854
TACGACG4800.016.9582395
ATACGTA800.001988818416.49990825
TCTATAC2007.2759576E-1116.4999083
ACGACGG5100.016.3920656
CGTTTAT5400.016.29620431
CCTATTC6350.016.2833733
TAGATCG953.3473363E-416.2104345
ATACCGT4500.016.1332426
CGACGGT5200.016.0768347
CTACGAC5100.015.9606944
ACCGTCG4000.015.949918
TACACTA1259.444653E-615.83991055
TGGTCGG5150.015.80573637
ATTACAC2101.5097612E-1015.7141983
CTATTCC6300.015.7141974
GTATAAT2252.5465852E-1115.648221
GTATAGA1704.4796252E-815.533161
CATCGTT5400.015.48139428
ATCGTTT5300.015.35840529
Produced by FastQC (version 0.11.5)