##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062694_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1619828
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27285427835548	32.0	32.0	32.0	32.0	32.0
2	31.39330101714503	32.0	32.0	32.0	32.0	32.0
3	31.47250263608235	32.0	32.0	32.0	32.0	32.0
4	31.58351010107246	32.0	32.0	32.0	32.0	32.0
5	31.50850151991446	32.0	32.0	32.0	32.0	32.0
6	35.0955836051729	36.0	36.0	36.0	36.0	36.0
7	35.11469365883291	36.0	36.0	36.0	36.0	36.0
8	35.05220492546122	36.0	36.0	36.0	36.0	36.0
9	35.186109883271556	36.0	36.0	36.0	36.0	36.0
10	35.05626091165235	36.0	36.0	36.0	36.0	36.0
11	35.19698140790257	36.0	36.0	36.0	36.0	36.0
12	35.098160421970725	36.0	36.0	36.0	36.0	36.0
13	35.14909175542095	36.0	36.0	36.0	36.0	36.0
14	35.10956163246962	36.0	36.0	36.0	36.0	36.0
15	35.0640845818198	36.0	36.0	36.0	36.0	36.0
16	35.0770810234173	36.0	36.0	36.0	36.0	36.0
17	35.04403553957581	36.0	36.0	36.0	36.0	36.0
18	35.06094968107725	36.0	36.0	36.0	36.0	36.0
19	35.04621046185151	36.0	36.0	36.0	36.0	36.0
20	35.04099632800519	36.0	36.0	36.0	36.0	36.0
21	35.03598159804621	36.0	36.0	36.0	36.0	36.0
22	35.01699563163496	36.0	36.0	36.0	36.0	36.0
23	34.974852885615014	36.0	36.0	36.0	36.0	36.0
24	34.95043177423776	36.0	36.0	36.0	32.0	36.0
25	34.92200838607556	36.0	36.0	36.0	32.0	36.0
26	34.880157029017894	36.0	36.0	36.0	32.0	36.0
27	34.85741942971723	36.0	36.0	36.0	32.0	36.0
28	34.84864689337387	36.0	36.0	36.0	32.0	36.0
29	34.8196746815094	36.0	36.0	36.0	32.0	36.0
30	34.81447721609949	36.0	36.0	36.0	32.0	36.0
31	34.80293648461441	36.0	36.0	36.0	32.0	36.0
32	34.776455895317284	36.0	36.0	36.0	32.0	36.0
33	34.751757594016155	36.0	36.0	36.0	32.0	36.0
34	34.73183387371993	36.0	36.0	36.0	32.0	36.0
35	34.70853201697958	36.0	36.0	36.0	32.0	36.0
36	34.67399995555083	36.0	36.0	36.0	32.0	36.0
37	34.67695952903642	36.0	36.0	36.0	32.0	36.0
38	34.63359319631467	36.0	36.0	36.0	32.0	36.0
39	34.62695421983075	36.0	36.0	36.0	32.0	36.0
40	34.61307743785142	36.0	36.0	36.0	32.0	36.0
41	34.5762827905185	36.0	36.0	36.0	32.0	36.0
42	34.55187711287865	36.0	36.0	36.0	32.0	36.0
43	34.53733421079275	36.0	36.0	36.0	32.0	36.0
44	34.506772941324634	36.0	36.0	36.0	32.0	36.0
45	34.47538627557988	36.0	36.0	36.0	32.0	36.0
46	34.47079196062792	36.0	36.0	36.0	32.0	36.0
47	34.448356862580475	36.0	36.0	36.0	32.0	36.0
48	34.403507656368454	36.0	36.0	36.0	32.0	36.0
49	34.393927626883844	36.0	36.0	36.0	32.0	36.0
50	33.86891447733957	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	9.0
21	43.0
22	128.0
23	429.0
24	1090.0
25	2537.0
26	5321.0
27	10032.0
28	16487.0
29	25723.0
30	37172.0
31	53473.0
32	78877.0
33	128798.0
34	285173.0
35	974534.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1654698558023	17.915672068471988	11.27241473417215	26.64644334155356
2	16.69557003639417	20.491984948433878	35.779572962744595	27.032872052427358
3	18.836560650640997	23.788351530850996	28.361828304167506	29.0132595143405
4	12.555623326978735	15.523970505437628	35.736104663222676	36.18430150436097
5	14.59778445612744	36.34268576663695	33.394286306941225	15.665243470294376
6	34.790390031472676	35.10808004356045	16.43857519795434	13.662954727012542
7	30.499657988379013	30.258768214896893	20.596199102620773	18.645374694103324
8	28.516378600924668	32.37358063546292	19.448249720494843	19.661791043117567
9	27.48044854145008	14.013401422867119	18.587837721041986	39.918312314640815
10	15.985956533656658	26.364404121919115	31.418891388468406	26.23074795595582
11	37.86179767234546	21.18342194356438	21.740765068883857	19.214015315206307
12	24.662248090538007	23.844630417550505	28.311215758710183	23.1819057332013
13	29.272120249804303	19.14968749768494	25.333553932886705	26.24463831962406
14	23.46298495889687	19.638196154159576	24.80849818622718	32.090320700716376
15	25.434799250290773	26.935020261410468	22.11543447822855	25.514746010070205
16	26.095486681301967	25.628276582451964	23.54793224959687	24.7283044866492
17	24.366537681778556	25.747857179898116	25.00160510869055	24.884000029632773
18	25.25447146240218	24.58495593359295	26.0711013761955	24.089471227809373
19	25.60432342199295	25.103714715389536	25.23872905024484	24.05323281237267
20	25.68068955469346	24.246895349382775	24.962959030218023	25.109456065705743
21	26.791239563706764	24.28683786179767	24.410678170768747	24.511244403726813
22	25.831137626957922	24.359623367419257	24.774852638675217	25.034386366947604
23	24.314309914385973	24.128611185878995	25.5290685183859	26.02801038134913
24	24.595574344930448	25.126803586553635	25.18273545092442	25.0948866175915
25	24.82152426060051	24.36838973026766	25.274597055983723	25.53548895314811
26	24.497662714806758	25.271633778401164	25.68383803712493	24.54686546966715
27	25.446405420822458	24.68367011806192	24.756949503280595	25.11297495783503
28	24.389503083043383	24.69996814476599	25.87645107999121	25.034077692199418
29	24.489884111152545	24.800163968026233	25.59561879409419	25.114333126727033
30	24.46339981775843	25.06216709428408	25.853238738927836	24.62119434902965
31	25.10001061841134	24.867702002928706	24.694350264349055	25.337937114310904
32	24.713055954088954	24.736576969900508	24.95388399262144	25.5964830833891
33	24.281652126028195	24.46704217978699	25.50110258620051	25.750203107984305
34	24.974503465800073	24.592549332398256	25.690073267038226	24.742873934763445
35	25.6470440071415	24.508095921295347	25.453196265282486	24.39166380628067
36	24.53729655247347	25.00790207355349	25.165264460177255	25.289536913795786
37	25.542403267507414	24.81399259674484	24.956353390606903	24.687250745140844
38	24.58625236753532	24.529888358517077	25.554811992384373	25.329047281563227
39	25.078588590887428	24.478771820217947	24.965490163153127	25.477149425741498
40	25.2497796062298	24.589586054815697	25.670935432650875	24.489698906303634
41	24.16713379445225	25.059882901147528	25.93763041508111	24.83535288931911
42	25.554379847736918	25.004136241625652	25.411710379126674	24.029773531510752
43	24.66144553619273	23.932849660581248	25.731188743496226	25.674516059729797
44	24.19682830522747	24.650703654955958	25.542218062658506	25.610249977158066
45	24.400985783675797	24.858688700281757	25.619139809905743	25.1211857061367
46	24.03974254024815	24.56214432908226	25.830613905444167	25.567499225225426
47	24.29044848026887	24.333354323242126	26.2843046104952	25.091892585993804
48	24.977405008432992	25.19279824771519	25.239593339539752	24.590203404312064
49	23.885437219260318	25.26632457273241	25.535797627896294	25.312440580110973
50	23.833115408692045	25.354451650979797	25.53440925136404	25.278023688964126
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	37.0
1	42.5
2	48.0
3	145.5
4	243.0
5	300.5
6	358.0
7	316.5
8	275.0
9	362.0
10	449.0
11	654.5
12	860.0
13	1468.0
14	2076.0
15	2789.0
16	3502.0
17	4012.5
18	4523.0
19	4785.0
20	5047.0
21	6140.5
22	7234.0
23	7821.5
24	8409.0
25	10167.5
26	11926.0
27	14640.5
28	17355.0
29	19537.0
30	21719.0
31	23667.5
32	25616.0
33	29229.0
34	32842.0
35	37612.5
36	42383.0
37	50887.0
38	59391.0
39	64704.5
40	70018.0
41	76791.5
42	83565.0
43	85733.0
44	87901.0
45	96130.0
46	104359.0
47	111873.0
48	119387.0
49	123323.0
50	127259.0
51	120968.0
52	114677.0
53	111718.5
54	108760.0
55	110505.5
56	112251.0
57	109647.5
58	107044.0
59	98225.0
60	89406.0
61	78596.0
62	67786.0
63	59484.0
64	51182.0
65	44014.0
66	36846.0
67	33306.5
68	29767.0
69	27371.5
70	24976.0
71	19389.5
72	13803.0
73	12255.0
74	10707.0
75	7872.0
76	5037.0
77	4445.0
78	3853.0
79	3339.5
80	2826.0
81	2268.0
82	1710.0
83	1424.0
84	1138.0
85	951.0
86	764.0
87	563.0
88	362.0
89	229.5
90	97.0
91	63.0
92	29.0
93	20.5
94	12.0
95	9.5
96	7.0
97	4.5
98	2.0
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01518679761061051
2	0.0038893018271075695
3	6.173494963662809E-4
4	2.4693979854651234E-4
5	0.0
6	6.173494963662809E-4
7	0.0
8	6.173494963662808E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.2346989927325617E-4
47	1.2346989927325617E-4
48	0.0
49	0.0
50	1.2346989927325617E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1619828.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.42216478334395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.62458254865807	45.49013482148703
2	12.292500999764348	13.871390340162487
3	3.317968158324687	5.616208385246512
4	1.280547084882505	2.8900495454428574
5	0.6457836874113564	1.821825681275952
6	0.3752286126760747	1.2702726369501018
7	0.2589744258719696	1.0228328411854115
8	0.17648686663746235	0.796621685721196
9	0.13189164294454114	0.6697450810586578
>10	0.7210875943078212	7.617438605027902
>50	0.08134084480109535	3.247037606424077
>100	0.0808779988990772	9.777036881313276
>500	0.009876363223194682	3.6963351789544325
>1k	0.0028531715978117973	2.213070709750051
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2034	0.1255688875609015	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2010	0.12408724876962246	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1770	0.10927086085683173	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1734	0.1070484026699131	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1666	0.1028504260946224	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1639	0.10118358245443343	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1636	0.10099837760552356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.173494963662808E-5	0.0
5	0.0	0.0	0.0	6.173494963662808E-5	0.0
6	0.0	0.0	0.0	6.173494963662808E-5	0.0
7	0.0	0.0	0.0	1.2346989927325617E-4	0.0
8	0.0	0.0	0.0	1.2346989927325617E-4	0.0
9	0.0	0.0	0.0	1.8520484890988427E-4	0.0
10	0.0	0.0	0.0	1.8520484890988427E-4	0.0
11	0.0	0.0	0.0	1.8520484890988427E-4	0.0
12	0.0	0.0	0.0	2.4693979854651234E-4	6.173494963662808E-5
13	0.0	0.0	0.0	3.0867474818314043E-4	6.173494963662808E-5
14	0.0	0.0	0.0	3.7040969781976853E-4	6.173494963662808E-5
15	0.0	0.0	0.0	4.3214464745639663E-4	6.173494963662808E-5
16	0.0	0.0	0.0	5.556145467296528E-4	6.173494963662808E-5
17	0.0	0.0	0.0	5.556145467296528E-4	6.173494963662808E-5
18	0.0	0.0	0.0	7.408193956395371E-4	6.173494963662808E-5
19	0.0	0.0	0.0	9.260242445494213E-4	6.173494963662808E-5
20	0.0	0.0	0.0	0.0010494941438226775	1.2346989927325617E-4
21	0.0	0.0	0.0	0.0012346989927325617	1.8520484890988427E-4
22	0.0	0.0	1.2346989927325617E-4	0.001543373740915702	1.8520484890988427E-4
23	0.0	0.0	1.2346989927325617E-4	0.0027163377840116357	1.8520484890988427E-4
24	0.0	0.0	1.2346989927325617E-4	0.004012771726380825	1.8520484890988427E-4
25	0.0	0.0	1.2346989927325617E-4	0.0045683862731104785	1.8520484890988427E-4
26	0.0	0.0	1.2346989927325617E-4	0.006420434762209321	2.4693979854651234E-4
27	0.0	0.0	1.2346989927325617E-4	0.008581157999491305	2.4693979854651234E-4
28	0.0	0.0	1.2346989927325617E-4	0.016544966502616328	2.4693979854651234E-4
29	0.0	0.0	1.2346989927325617E-4	0.03358381260232568	2.4693979854651234E-4
30	0.0	0.0	1.2346989927325617E-4	0.06679721550683158	2.4693979854651234E-4
31	0.0	0.0	1.2346989927325617E-4	0.13353269606402654	2.4693979854651234E-4
32	0.0	0.0	1.2346989927325617E-4	0.21107179280763141	2.4693979854651234E-4
33	0.0	0.0	1.2346989927325617E-4	0.29151243218415784	2.4693979854651234E-4
34	0.0	0.0	1.2346989927325617E-4	0.38436179643764645	2.4693979854651234E-4
35	0.0	0.0	1.2346989927325617E-4	0.4883851865753648	2.4693979854651234E-4
36	0.0	0.0	1.2346989927325617E-4	0.6392654034872839	2.4693979854651234E-4
37	0.0	0.0	1.2346989927325617E-4	0.8516953651869211	2.4693979854651234E-4
38	0.0	0.0	1.2346989927325617E-4	1.147344038996733	2.4693979854651234E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	185	0.0	21.405285	4
TACGACG	480	0.0	16.958239	5
ATACGTA	80	0.0019888184	16.499908	25
TCTATAC	200	7.2759576E-11	16.499908	3
ACGACGG	510	0.0	16.392065	6
CGTTTAT	540	0.0	16.296204	31
CCTATTC	635	0.0	16.283373	3
TAGATCG	95	3.3473363E-4	16.210434	5
ATACCGT	450	0.0	16.133242	6
CGACGGT	520	0.0	16.076834	7
CTACGAC	510	0.0	15.960694	4
ACCGTCG	400	0.0	15.94991	8
TACACTA	125	9.444653E-6	15.8399105	5
TGGTCGG	515	0.0	15.805736	37
ATTACAC	210	1.5097612E-10	15.714198	3
CTATTCC	630	0.0	15.714197	4
GTATAAT	225	2.5465852E-11	15.64822	1
GTATAGA	170	4.4796252E-8	15.53316	1
CATCGTT	540	0.0	15.481394	28
ATCGTTT	530	0.0	15.358405	29
>>END_MODULE