FastQCFastQC Report
Thu 2 Feb 2017
SRR4062693_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062693_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2312435
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47350.20476251224358738No Hit
GTACATGGAAGCAGTGGTATCAACG46830.2025138003879028No Hit
TCCATGTACTCTGCGTTGATACCAC38830.1679182333773706No Hit
TATCAACGCAGAGTACTTTTTTTTT29440.12731168659875844No Hit
GTCTTGCGCCGGTCCAAGAATTTCA28770.12441430786162637No Hit
GCGCAAGACGGACCAGAGCGAAAGC27520.11900875051623072No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG26280.11364643762959825No Hit
GAATAGGACCGCGGTTCTATTTTGT25310.1094517251295712No Hit
GGGTAGGCACACGCTGAGCCAGTCA25190.10893279162441322No Hit
GTCCTATTCCATTATTCCTAGCTGC23410.1012352779645698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAGA4600.013.0035782
AAGACGG4750.012.7991975
CGACCAT2850.012.66861610
ACGGTAT3500.012.4876369
CCGACCA2950.012.2391719
AACTATC701.0899975E-412.21597
GTATAGA1650.012.0838721
AAGACCG1353.7289283E-1011.96221355
CGGTCCA4700.011.92736710
CAAGACG5150.011.8038074
CGGACCA5100.011.7371019
GCGCAAG5350.011.5353371
GACCATA2900.011.46703311
CGGCGTC3250.011.40027314
TCGGCGT3250.011.40027313
GTCCTAC7850.011.3691741
TCCTACA8200.011.3472962
CGCCGGT4700.011.3206457
GGGTAGG6100.011.2065871
AATCGTA855.3239237E-511.17673913
Produced by FastQC (version 0.11.5)