##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062693_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2312435 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.05001610856089 32.0 32.0 32.0 32.0 32.0 2 30.579424286520485 32.0 32.0 32.0 32.0 32.0 3 30.573981538940554 32.0 32.0 32.0 32.0 32.0 4 30.67307578375176 32.0 32.0 32.0 32.0 32.0 5 30.388482270853018 32.0 32.0 32.0 32.0 32.0 6 34.03842616116777 36.0 36.0 36.0 32.0 36.0 7 33.90701965676873 36.0 36.0 36.0 32.0 36.0 8 33.87763115503787 36.0 36.0 36.0 32.0 36.0 9 34.10934491131643 36.0 36.0 36.0 32.0 36.0 10 33.666034720975944 36.0 36.0 36.0 27.0 36.0 11 34.09053962597868 36.0 36.0 36.0 32.0 36.0 12 33.80908522834155 36.0 36.0 36.0 32.0 36.0 13 33.96177622289924 36.0 36.0 36.0 32.0 36.0 14 33.84616216239591 36.0 36.0 36.0 32.0 36.0 15 33.765470164566786 36.0 36.0 36.0 32.0 36.0 16 33.77000002162223 36.0 36.0 36.0 32.0 36.0 17 33.66492160860738 36.0 36.0 36.0 27.0 36.0 18 33.689307591348516 36.0 36.0 36.0 27.0 36.0 19 33.68186694977372 36.0 36.0 36.0 27.0 36.0 20 33.66368135753005 36.0 36.0 36.0 27.0 36.0 21 33.63914920851829 36.0 36.0 36.0 27.0 36.0 22 33.58871925048704 36.0 36.0 36.0 27.0 36.0 23 33.536547405656805 36.0 36.0 36.0 27.0 36.0 24 33.50099959566431 36.0 36.0 36.0 27.0 36.0 25 33.002535422617285 36.0 36.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 14.0 4 96.0 5 228.0 6 401.0 7 124.0 8 354.0 9 451.0 10 277.0 11 86.0 12 152.0 13 139.0 14 480.0 15 600.0 16 941.0 17 1263.0 18 1859.0 19 2759.0 20 4294.0 21 6387.0 22 9687.0 23 14306.0 24 19750.0 25 26826.0 26 35925.0 27 44830.0 28 57888.0 29 75202.0 30 95040.0 31 127265.0 32 177086.0 33 245032.0 34 512623.0 35 850069.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.77593831156896 17.203348232144673 11.741906745117882 25.27880671116849 2 16.48162555370704 19.198060517157945 38.209559114483895 26.11075481465112 3 19.873936951177058 23.13734817835651 27.40731771131778 29.581397159148654 4 12.652399927165497 15.012255140476737 35.14788129013953 37.18746364221823 5 14.902592037265672 36.33044041013697 32.99139390677872 15.775573645818644 6 35.21708468357771 34.4050776399531 16.484764051555178 13.89307362491401 7 31.157574233796264 29.938364430958252 19.745149060450288 19.158912274795192 8 28.116978848616064 33.49243257796181 18.781343359168385 19.609245214253747 9 26.865696192833084 15.130578496655836 18.422106337140168 39.581618973370915 10 16.311239018400553 26.470963895536094 30.719599733120777 26.498197352942576 11 37.604172800421196 21.285282799278153 21.547966249051793 19.56257815124886 12 25.123704978727552 23.435289532655645 28.03853940004114 23.402466088575665 13 29.58309537407196 19.36798597487237 24.99726139379252 26.05165725726315 14 23.49928273866097 19.85482439978887 24.538304959690045 32.10758790186012 15 25.232213288999144 27.214609499811665 22.13440532010235 25.418771891086838 16 25.946367186485293 25.892509243304556 23.6377057555265 24.523417814683647 17 24.031231963532402 26.246894967303252 24.925472720526056 24.79640034863829 18 25.11217422707822 24.625371424216468 26.32056410580621 23.941890242899095 19 25.546215721867338 25.204603558413126 24.98752892596339 24.261651793756148 20 25.688353839332724 24.273067517388533 25.1806398426026 24.857938800676145 21 26.586192069483495 24.323986103647595 24.179012316276687 24.910809510592223 22 25.59896331855866 24.566223137711106 24.825999845814426 25.008813697915812 23 24.046676733823872 24.49796657571003 25.339181927273092 26.116174763193005 24 24.532333196061288 25.343883794233967 25.138841796349777 24.984941213354965 25 24.865414927337916 24.788768988273606 24.976746401538115 25.369069682850366 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 338.0 1 338.0 2 716.5 3 1095.0 4 1095.0 5 1095.0 6 2461.5 7 3828.0 8 3828.0 9 3828.0 10 4215.5 11 4603.0 12 4603.0 13 4603.0 14 5347.5 15 6092.0 16 6092.0 17 6092.0 18 10005.0 19 13918.0 20 13918.0 21 13918.0 22 22449.0 23 30980.0 24 30980.0 25 30980.0 26 46333.5 27 61687.0 28 61687.0 29 61687.0 30 78275.5 31 94864.0 32 94864.0 33 94864.0 34 117187.0 35 139510.0 36 139510.0 37 139510.0 38 159571.5 39 179633.0 40 179633.0 41 179633.0 42 208564.5 43 237496.0 44 237496.0 45 237496.0 46 275133.5 47 312771.0 48 312771.0 49 312771.0 50 313098.0 51 313425.0 52 313425.0 53 313425.0 54 291525.5 55 269626.0 56 269626.0 57 269626.0 58 249462.5 59 229299.0 60 229299.0 61 229299.0 62 202460.5 63 175622.0 64 175622.0 65 175622.0 66 144660.0 67 113698.0 68 113698.0 69 113698.0 70 85488.0 71 57278.0 72 57278.0 73 57278.0 74 44704.5 75 32131.0 76 32131.0 77 32131.0 78 26110.5 79 20090.0 80 20090.0 81 20090.0 82 14011.0 83 7932.0 84 7932.0 85 7932.0 86 5862.0 87 3792.0 88 3792.0 89 3792.0 90 2587.5 91 1383.0 92 1383.0 93 1383.0 94 834.5 95 286.0 96 286.0 97 286.0 98 672.0 99 1058.0 100 1058.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007351557989738091 2 0.0028541342783689055 3 0.004324445876316523 4 0.01487609381452884 5 0.035330722809506 6 0.04865001610856089 7 0.06893166726848539 8 0.09241340837688411 9 0.09911629948517472 10 0.12004661752654669 11 0.12156017358325748 12 0.1344902667534439 13 0.12393861881523156 14 0.12783062010391644 15 0.11697626095436195 16 0.1148140380162037 17 0.12445755232038955 18 0.1332361774493121 19 0.13366862203694374 20 0.12398186327399471 21 0.1319820881451803 22 0.15260969497521013 23 0.1416688469081293 24 0.13630653402149684 25 0.1347064890472597 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2312435.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.205259650274 #Duplication Level Percentage of deduplicated Percentage of total 1 76.97614136451627 38.64607164081736 2 13.353254071166726 13.40807175638008 3 4.252352730741309 6.404714189142574 4 1.8087512872235871 3.6323531207131006 5 0.97769188345229 2.4542637433343826 6 0.5865651006712126 1.7669191908592408 7 0.3695463855060491 1.2987220566006015 8 0.27492526044476295 1.1042155268038825 9 0.19779611850862375 0.8937364938787641 >10 1.0230635724793542 9.173523672135659 >50 0.08248484813720385 2.8667838422449203 >100 0.07793008529261289 8.427169699331905 >500 0.013285765249819972 4.62886957709025 >1k 0.006211526610305407 5.294585490667131 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4735 0.20476251224358738 No Hit GTACATGGAAGCAGTGGTATCAACG 4683 0.2025138003879028 No Hit TCCATGTACTCTGCGTTGATACCAC 3883 0.1679182333773706 No Hit TATCAACGCAGAGTACTTTTTTTTT 2944 0.12731168659875844 No Hit GTCTTGCGCCGGTCCAAGAATTTCA 2877 0.12441430786162637 No Hit GCGCAAGACGGACCAGAGCGAAAGC 2752 0.11900875051623072 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 2628 0.11364643762959825 No Hit GAATAGGACCGCGGTTCTATTTTGT 2531 0.1094517251295712 No Hit GGGTAGGCACACGCTGAGCCAGTCA 2519 0.10893279162441322 No Hit GTCCTATTCCATTATTCCTAGCTGC 2341 0.1012352779645698 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.3244458763165234E-5 0.0 4 0.0 0.0 0.0 4.3244458763165234E-5 0.0 5 0.0 0.0 0.0 4.3244458763165234E-5 0.0 6 0.0 0.0 0.0 4.3244458763165234E-5 4.3244458763165234E-5 7 0.0 0.0 0.0 4.3244458763165234E-5 4.3244458763165234E-5 8 0.0 0.0 0.0 4.3244458763165234E-5 4.3244458763165234E-5 9 0.0 0.0 0.0 8.648891752633047E-5 4.3244458763165234E-5 10 0.0 0.0 0.0 8.648891752633047E-5 4.3244458763165234E-5 11 0.0 0.0 0.0 1.297333762894957E-4 4.3244458763165234E-5 12 0.0 0.0 0.0 1.7297783505266094E-4 2.1622229381582618E-4 13 0.0 0.0 0.0 1.7297783505266094E-4 3.0271121134215666E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAAGA 460 0.0 13.003578 2 AAGACGG 475 0.0 12.799197 5 CGACCAT 285 0.0 12.668616 10 ACGGTAT 350 0.0 12.487636 9 CCGACCA 295 0.0 12.239171 9 AACTATC 70 1.0899975E-4 12.2159 7 GTATAGA 165 0.0 12.083872 1 AAGACCG 135 3.7289283E-10 11.9622135 5 CGGTCCA 470 0.0 11.927367 10 CAAGACG 515 0.0 11.803807 4 CGGACCA 510 0.0 11.737101 9 GCGCAAG 535 0.0 11.535337 1 GACCATA 290 0.0 11.467033 11 CGGCGTC 325 0.0 11.400273 14 TCGGCGT 325 0.0 11.400273 13 GTCCTAC 785 0.0 11.369174 1 TCCTACA 820 0.0 11.347296 2 CGCCGGT 470 0.0 11.320645 7 GGGTAGG 610 0.0 11.206587 1 AATCGTA 85 5.3239237E-5 11.176739 13 >>END_MODULE