FastQCFastQC Report
Thu 2 Feb 2017
SRR4062693_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062693_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2312435
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC33480.14478244793907719No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26810.11593839394404601No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA25350.10962470296462387No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT25350.10962470296462387No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC25290.10936523621204487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC3900.021.439481
CGCAATA7350.020.95225536
TCTAGCG7850.019.05721328
CTAGCGG7900.018.93659829
GTCCTAC8600.018.677541
CGGTCCA9000.018.33322510
TAGCGGC8050.018.31045230
GGCGTTA7050.018.09918442
CGAATGC8200.017.97550243
CAATACG8350.017.91606138
AATACGA8600.017.65105839
ATACGAA8650.017.29469540
CCGGTCC9700.017.2370139
TAGGACG5750.017.217294
CGCCGGT9400.017.0850077
TACGAAT8650.017.04036141
GCAATAC9050.017.01647437
TCTTGCG9500.016.9066262
TGTAGGA11350.016.8647962
GCCGGTC9450.016.7618068
Produced by FastQC (version 0.11.5)