##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062693_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2312435
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.197457225824724	32.0	32.0	32.0	32.0	32.0
2	31.308607160849927	32.0	32.0	32.0	32.0	32.0
3	31.43622112621544	32.0	32.0	32.0	32.0	32.0
4	31.55859689029097	32.0	32.0	32.0	32.0	32.0
5	31.476249494580387	32.0	32.0	32.0	32.0	32.0
6	35.05333901277225	36.0	36.0	36.0	36.0	36.0
7	35.07426111436646	36.0	36.0	36.0	36.0	36.0
8	35.0048982998441	36.0	36.0	36.0	36.0	36.0
9	35.14618529818136	36.0	36.0	36.0	36.0	36.0
10	35.015123019674064	36.0	36.0	36.0	36.0	36.0
11	35.159085552674995	36.0	36.0	36.0	36.0	36.0
12	35.06966249862158	36.0	36.0	36.0	36.0	36.0
13	35.113808171905376	36.0	36.0	36.0	36.0	36.0
14	35.06789034070147	36.0	36.0	36.0	36.0	36.0
15	35.02787883767544	36.0	36.0	36.0	36.0	36.0
16	35.034114256184495	36.0	36.0	36.0	36.0	36.0
17	35.01230304851812	36.0	36.0	36.0	36.0	36.0
18	35.02824987513163	36.0	36.0	36.0	36.0	36.0
19	35.0070557658918	36.0	36.0	36.0	36.0	36.0
20	35.00354604561858	36.0	36.0	36.0	36.0	36.0
21	34.988570057104305	36.0	36.0	36.0	36.0	36.0
22	34.972928103925085	36.0	36.0	36.0	36.0	36.0
23	34.92812943931397	36.0	36.0	36.0	32.0	36.0
24	34.900769967588275	36.0	36.0	36.0	32.0	36.0
25	34.86977493421437	36.0	36.0	36.0	32.0	36.0
26	34.820226298252706	36.0	36.0	36.0	32.0	36.0
27	34.799022242787366	36.0	36.0	36.0	32.0	36.0
28	34.77795743447924	36.0	36.0	36.0	32.0	36.0
29	34.74706964736306	36.0	36.0	36.0	32.0	36.0
30	34.732904060006014	36.0	36.0	36.0	32.0	36.0
31	34.729023734721196	36.0	36.0	36.0	32.0	36.0
32	34.68573862616679	36.0	36.0	36.0	32.0	36.0
33	34.67068090562545	36.0	36.0	36.0	32.0	36.0
34	34.654542938504214	36.0	36.0	36.0	32.0	36.0
35	34.6183455967411	36.0	36.0	36.0	32.0	36.0
36	34.5882241014342	36.0	36.0	36.0	32.0	36.0
37	34.57657836868928	36.0	36.0	36.0	32.0	36.0
38	34.52677632019927	36.0	36.0	36.0	32.0	36.0
39	34.490459191285375	36.0	36.0	36.0	32.0	36.0
40	34.47914903554046	36.0	36.0	36.0	32.0	36.0
41	34.44950625639207	36.0	36.0	36.0	32.0	36.0
42	34.4012026283982	36.0	36.0	36.0	32.0	36.0
43	34.404610724193326	36.0	36.0	36.0	32.0	36.0
44	34.37146773855265	36.0	36.0	36.0	32.0	36.0
45	34.31128701995948	36.0	36.0	36.0	32.0	36.0
46	34.316949016945344	36.0	36.0	36.0	32.0	36.0
47	34.27848826021056	36.0	36.0	36.0	32.0	36.0
48	34.242694389247696	36.0	36.0	36.0	32.0	36.0
49	34.23087092177726	36.0	36.0	36.0	32.0	36.0
50	33.70358647918752	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	23.0
21	95.0
22	312.0
23	789.0
24	2033.0
25	4620.0
26	9064.0
27	16734.0
28	27160.0
29	40068.0
30	56795.0
31	80605.0
32	119023.0
33	191231.0
34	417391.0
35	1346487.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.215635433025575	17.761191383129137	12.271779086177792	25.7513940976675
2	15.951147618400988	19.584631072923955	37.80606256654474	26.65815874213031
3	19.402149869898146	23.131534553210294	27.83773182974193	29.628583747149627
4	12.47111805714419	15.425627834949454	35.32529186816817	36.77796223973818
5	14.727944667826195	36.43368848581192	32.90186876684913	15.936498079512756
6	35.58430168822627	34.58340522749475	16.085437507865112	13.746855576413871
7	31.29486073980922	29.806558803408013	19.678399469995682	19.220180986787085
8	28.25692397840372	33.50835807276745	18.58923602176926	19.64548192705957
9	26.993666849014136	14.741819770069212	18.32064468839124	39.94386869252541
10	16.308220555388584	26.490993260351104	30.647218192078913	26.553567992181403
11	37.93399598258978	21.07263555516155	21.470657553617723	19.522710908630945
12	25.241487868848207	23.27075139409324	28.01190087505162	23.47585986200693
13	29.800837645166244	19.093163699736426	25.070931723486282	26.035066931611052
14	23.594609145770583	19.623643475384174	24.499975134436212	32.28177224440903
15	25.32161985093635	27.113540488705627	22.07011224099272	25.494727419365297
16	26.08341423650827	25.803709077228117	23.47689772901725	24.635978957246365
17	24.155965464975232	26.12674518418896	24.95862586407834	24.758663486757467
18	25.32979305364259	24.50637531433316	26.244586334318587	23.919245297705665
19	25.706106333799656	25.037071312274723	25.00113516704253	24.25568718688309
20	25.811103879676615	24.073195570902534	25.247023159569892	24.86867738985096
21	26.738005608806304	24.21222650582611	24.229610778248905	24.820157107118686
22	25.68400841537167	24.359690110208504	25.0102165033828	24.946084971037024
23	24.237524514202562	24.141521815748334	25.45615336214856	26.16480030790055
24	24.7096675149788	25.05960167529033	25.179864515110694	25.05086629462017
25	25.060034119877965	24.505899625286766	25.02011948443956	25.413946770395707
26	24.488904552992842	25.341339324132356	25.64184506807759	24.527911054797215
27	25.414206237148285	24.764847444360598	24.925284386371942	24.89566193211917
28	24.588626274900697	24.466157967683415	25.520587605705675	25.42462815171021
29	24.316964584950497	24.870839612789116	25.638385511376537	25.173810290883853
30	24.61202152709157	24.762858199257494	25.89257643998642	24.732543833664515
31	25.225919863693463	24.69669417734985	24.580453072194462	25.496932886762224
32	24.74287925930891	24.822622041268186	24.798188922067	25.636309777355905
33	24.302650669099886	24.530030033276613	25.421946995266893	25.745372302356607
34	25.098348710342126	24.77241522464415	25.520674094623203	24.608561970390518
35	25.71553362581002	24.48341250672992	25.463764386890876	24.337289480569183
36	24.47649339332781	25.18116184887359	25.195778475935537	25.14656628186306
37	25.61710923766506	24.726662587272724	24.91023531472236	24.745992860339857
38	24.673688125287846	24.644757582375288	25.25069893856476	25.43085535377211
39	25.36166421975104	24.385680029925165	24.919316651062626	25.33333909926117
40	25.655294094752932	24.70698635853548	25.109073336115394	24.52864621059619
41	24.472039214075206	25.008659702867327	25.82563401782104	24.693667065236426
42	25.8668892314811	25.189032340368485	25.07309394642444	23.870984481725973
43	24.943490303511233	24.138667681469965	25.312711492431138	25.60513052258766
44	24.44886018417815	24.869412545649933	25.28788917310108	25.393838097070837
45	24.74002512503054	24.999621610985823	25.128014409053662	25.132338854929976
46	24.622053587761105	24.522980361359974	25.365037918969257	25.489928131909668
47	24.928235789648483	24.47875269088761	25.796714630558103	24.796296888905804
48	25.52473907374694	25.417406327096764	24.427886621678017	24.629967977478287
49	24.523024430956976	25.101029866785446	25.059212475161463	25.316733227096112
50	24.45595227541531	25.763880930707238	24.541446570390086	25.238720223487366
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	25.0
2	27.0
3	104.0
4	181.0
5	232.5
6	284.0
7	271.0
8	258.0
9	323.0
10	388.0
11	652.5
12	917.0
13	1768.5
14	2620.0
15	3658.5
16	4697.0
17	5261.5
18	5826.0
19	6180.5
20	6535.0
21	7384.5
22	8234.0
23	9230.5
24	10227.0
25	11738.5
26	13250.0
27	17039.0
28	20828.0
29	24437.5
30	28047.0
31	31727.0
32	35407.0
33	41255.5
34	47104.0
35	54246.5
36	61389.0
37	72071.5
38	82754.0
39	90099.5
40	97445.0
41	106404.5
42	115364.0
43	119403.5
44	123443.0
45	137294.5
46	151146.0
47	163613.5
48	176081.0
49	180005.0
50	183929.0
51	175693.5
52	167458.0
53	165442.0
54	163426.0
55	165987.5
56	168549.0
57	162486.0
58	156423.0
59	144421.0
60	132419.0
61	116458.5
62	100498.0
63	87525.0
64	74552.0
65	63380.0
66	52208.0
67	45072.0
68	37936.0
69	35152.0
70	32368.0
71	24851.5
72	17335.0
73	15468.0
74	13601.0
75	9794.0
76	5987.0
77	5324.5
78	4662.0
79	4071.5
80	3481.0
81	2749.5
82	2018.0
83	1756.5
84	1495.0
85	1221.5
86	948.0
87	697.0
88	446.0
89	290.0
90	134.0
91	84.5
92	35.0
93	25.5
94	16.0
95	18.0
96	20.0
97	16.0
98	12.0
99	8.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015827471907318477
2	0.003502801159816384
3	6.919113402106438E-4
4	1.7297783505266094E-4
5	4.3244458763165234E-5
6	3.459556701053219E-4
7	4.3244458763165234E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.7297783505266094E-4
47	4.3244458763165234E-5
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2312435.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.17560198566288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.30805213247842	39.81418556365408
2	13.960947747377888	14.568417060196511
3	4.384422631209149	6.8627967042870415
4	1.8657104938797353	3.8937827259657434
5	0.9455148393161613	2.466640296384901
6	0.5578945658563322	1.7465090890850508
7	0.38679972779422134	1.4127056051887825
8	0.27082185833201466	1.130423479147902
9	0.1887994213579162	0.8865651117504687
>10	0.9712403067761247	8.95449366099608
>50	0.07549141348798674	2.762084327922198
>100	0.06895189546384385	7.761509273620327
>500	0.010096244386295338	3.5857448434771193
>1k	0.005256722283773518	4.1541422583238115
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	3348	0.14478244793907719	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2681	0.11593839394404601	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2535	0.10962470296462387	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2535	0.10962470296462387	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2529	0.10936523621204487	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.3244458763165234E-5
2	0.0	0.0	0.0	0.0	4.3244458763165234E-5
3	0.0	0.0	0.0	0.0	4.3244458763165234E-5
4	0.0	0.0	0.0	0.0	4.3244458763165234E-5
5	0.0	4.3244458763165234E-5	0.0	0.0	4.3244458763165234E-5
6	0.0	4.3244458763165234E-5	0.0	0.0	8.648891752633047E-5
7	0.0	4.3244458763165234E-5	0.0	0.0	8.648891752633047E-5
8	0.0	4.3244458763165234E-5	0.0	0.0	8.648891752633047E-5
9	0.0	4.3244458763165234E-5	0.0	0.0	8.648891752633047E-5
10	0.0	4.3244458763165234E-5	0.0	0.0	8.648891752633047E-5
11	0.0	4.3244458763165234E-5	0.0	4.3244458763165234E-5	1.297333762894957E-4
12	0.0	4.3244458763165234E-5	0.0	8.648891752633047E-5	4.7568904639481757E-4
13	0.0	4.3244458763165234E-5	0.0	8.648891752633047E-5	4.7568904639481757E-4
14	0.0	4.3244458763165234E-5	0.0	8.648891752633047E-5	5.189335051579828E-4
15	0.0	4.3244458763165234E-5	0.0	1.7297783505266094E-4	6.054224226843133E-4
16	0.0	4.3244458763165234E-5	0.0	3.0271121134215666E-4	6.486668814474785E-4
17	0.0	4.3244458763165234E-5	0.0	3.892001288684871E-4	6.919113402106438E-4
18	0.0	4.3244458763165234E-5	0.0	6.054224226843133E-4	8.216447165001394E-4
19	0.0	4.3244458763165234E-5	0.0	6.054224226843133E-4	8.216447165001394E-4
20	0.0	4.3244458763165234E-5	0.0	7.784002577369742E-4	9.0813363402647E-4
21	0.0	4.3244458763165234E-5	0.0	9.946225515528003E-4	9.0813363402647E-4
22	0.0	4.3244458763165234E-5	0.0	0.0017297783505266094	9.0813363402647E-4
23	0.0	4.3244458763165234E-5	0.0	0.003675778994869045	9.0813363402647E-4
24	0.0	4.3244458763165234E-5	0.0	0.005146090592816663	9.0813363402647E-4
25	0.0	4.3244458763165234E-5	0.0	0.0064001798969484545	9.0813363402647E-4
26	0.0	4.3244458763165234E-5	0.0	0.007913735953659238	9.946225515528003E-4
27	0.0	4.3244458763165234E-5	0.0	0.010032714433054335	0.0010378670103159656
28	0.0	4.3244458763165234E-5	0.0	0.013492271134107553	0.0010378670103159656
29	0.0	4.3244458763165234E-5	0.0	0.020454628994977155	0.0010378670103159656
30	0.0	4.3244458763165234E-5	0.0	0.03169818827340012	0.0010378670103159656
31	0.0	4.3244458763165234E-5	0.0	0.06192606494885262	0.0010378670103159656
32	0.0	4.3244458763165234E-5	0.0	0.09634865412433215	0.0010378670103159656
33	0.0	4.3244458763165234E-5	0.0	0.12925768724310088	0.0010378670103159656
34	0.0	4.3244458763165234E-5	0.0	0.1669236108258178	0.0011243559278422961
35	0.0	4.3244458763165234E-5	0.0	0.2094761582487724	0.0011243559278422961
36	0.0	4.3244458763165234E-5	0.0	0.2715752010326777	0.0011243559278422961
37	0.0	4.3244458763165234E-5	0.0	0.36065878608479807	0.0011243559278422961
38	0.0	4.3244458763165234E-5	0.0	0.47434846817315945	0.0011676003866054614
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATAC	390	0.0	21.43948	1
CGCAATA	735	0.0	20.952255	36
TCTAGCG	785	0.0	19.057213	28
CTAGCGG	790	0.0	18.936598	29
GTCCTAC	860	0.0	18.67754	1
CGGTCCA	900	0.0	18.333225	10
TAGCGGC	805	0.0	18.310452	30
GGCGTTA	705	0.0	18.099184	42
CGAATGC	820	0.0	17.975502	43
CAATACG	835	0.0	17.916061	38
AATACGA	860	0.0	17.651058	39
ATACGAA	865	0.0	17.294695	40
CCGGTCC	970	0.0	17.237013	9
TAGGACG	575	0.0	17.21729	4
CGCCGGT	940	0.0	17.085007	7
TACGAAT	865	0.0	17.040361	41
GCAATAC	905	0.0	17.016474	37
TCTTGCG	950	0.0	16.906626	2
TGTAGGA	1135	0.0	16.864796	2
GCCGGTC	945	0.0	16.761806	8
>>END_MODULE