FastQCFastQC Report
Thu 2 Feb 2017
SRR4062692_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062692_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248612
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51852.0855791353595157No Hit
GTACATGGAAGCAGTGGTATCAACG46311.8627419432690295No Hit
GTACTTTTTTTTTTTTTTTTTTTTT44041.7714350071597509No Hit
TCCATGTACTCTGCGTTGATACCAC39671.5956590993194215No Hit
TATCAACGCAGAGTACTTTTTTTTT34071.3704085080366193No Hit
GAGTACTTTTTTTTTTTTTTTTTTT33351.3414477177288304No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23950.9633485109326984No Hit
GAGTACATGGAAGCAGTGGTATCAA22140.890544301964507No Hit
CATGTACTCTGCGTTGATACCACTG21940.8824996379901211No Hit
GGTATCAACGCAGAGTACTTTTTTT19010.7646453107653693No Hit
CCCATGTACTCTGCGTTGATACCAC15380.6186346596302673No Hit
GTACATGGGAAGCAGTGGTATCAAC15330.6166234936366708No Hit
GCTTCCATGTACTCTGCGTTGATAC12290.49434460122600676No Hit
GCGTTGATACCACTGCTTCCATGTA11730.4718195420977266No Hit
CATGGAAGCAGTGGTATCAACGCAG11440.46015477933486715No Hit
GTACATGGGGTGGTATCAACGCAAA10860.4368252538091484No Hit
GCAGAGTACTTTTTTTTTTTTTTTT9950.40022203272569307No Hit
GTATCAACGCAGAGTACATGGGAAG9310.3744791080076585No Hit
GTATCAACGCAGAGTACATGGAAGC9140.36764114362943057No Hit
ACTCTGCGTTGATACCACTGCTTCC8770.3527585152768169No Hit
CAGTGGTATCAACGCAGAGTACATG7880.31695976059080017No Hit
ACGCAGAGTACATGGAAGCAGTGGT7480.30087043264202856No Hit
GTACATGGGTGGTATCAACGCAAAA6610.26587614435345036No Hit
TATCAACGCAGAGTACATGGAAGCA6140.24697118401364376No Hit
GCGTTGATACCACTGCTTCCCATGT6060.24375331842388945No Hit
GTATCAACGCAGAGTACATGGGGTG5730.2304796228661529No Hit
ATCAACGCAGAGTACTTTTTTTTTT5690.22887069007127572No Hit
GAGTACATGGGAAGCAGTGGTATCA5550.22323942528920565No Hit
TATCAACGCAGAGTACATGGGAAGC5490.22082602609688995No Hit
GGTATCAACGCAGAGTACATGGGAA5420.2180103937058549No Hit
GTACATGGGAGTGGTATCAACGCAA4790.19266970218653967No Hit
ACGCAGAGTACATGGGAAGCAGTGG4710.18945183659678536No Hit
ATACCACTGCTTCCATGTACTCTGC4540.18261387221855743No Hit
AAGCAGTGGTATCAACGCAGAGTAC4040.16250221228259296No Hit
TATCAACGCAGAGTACATGGGGTGG3960.15928434669283864No Hit
GGTATCAACGCAGAGTACATGGAAG3930.15807764709668076No Hit
AAAAAGTACTCTGCGTTGATACCAC3740.15043521632101428No Hit
GCTTCCCATGTACTCTGCGTTGATA3720.1496307499235757No Hit
GTATCAACGCAGAGTACATGGGCAG3700.1488262835261371No Hit
GTACTCTGCGTTGATACCACTGCTT3560.14319501874406707No Hit
GTGGTATCAACGCAGAGTACATGGA3520.1415860859491899No Hit
GGTATCAACGCAGAGTACATGGGGT3380.13595482116711985No Hit
CCACTGCTTCCATGTACTCTGCGTT3270.13153025598120766No Hit
GTATCAACGCAGAGTACATGGGAGT3170.12750792399401478No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT3080.12388782520554116No Hit
GCGCAAGACGGACCAGAGCGAAAGC2900.11664762762859394No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT2860.11503869483371679No Hit
CTGCTTCCATGTACTCTGCGTTGAT2840.11423422843627822No Hit
GTCTTGCGCCGGTCCAAGAATTTCA2730.10980966325036604No Hit
ACGCAGAGTACATGGGGTGGTATCA2710.10900519685292745No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG2680.10779849725676958No Hit
GAAGCAGTGGTATCAACGCAGAGTA2640.10618956446189241No Hit
CAACGCAGAGTACTTTTTTTTTTTT2610.10498286486573455No Hit
GTTGATACCACTGCTTCCATGTACT2580.10377616526957668No Hit
ACATGGAAGCAGTGGTATCAACGCA2550.10256946567341882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGGTA250.00601224119.0032229
ATTACCG250.00601923918.99939715
CCATAAA453.5205714E-516.88835113
CATAAAC402.7500844E-416.62447214
GCTATTG350.00216085516.28847512
CGCTATT350.00216085516.28847511
GTAACTA350.002163764316.28519613
TAAAGTG350.00216667716.281925
AATATAC350.00216667716.281925
AAGACGG508.684324E-515.1964585
CAAGACG508.684324E-515.1964584
CAGCCCC456.735701E-414.777307515
GATTTAA456.735701E-414.77730751
CCGAGCC456.735701E-414.777307514
AGGCCCG400.00525667914.25241610
ACGCTAT400.00525667914.25241610
ATACGCT400.00525667914.2524168
TCGCGTA400.00525667914.2524169
CCGACCA602.5470901E-514.2524159
TAGTTAG400.00526370814.24954718
Produced by FastQC (version 0.11.5)