##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062692_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	248612
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.527436326484644	32.0	32.0	32.0	32.0	32.0
2	30.532548710440366	32.0	32.0	32.0	32.0	32.0
3	30.54946261644651	32.0	32.0	32.0	32.0	32.0
4	30.400077228774155	32.0	32.0	32.0	32.0	32.0
5	30.423342396988076	32.0	32.0	32.0	32.0	32.0
6	33.8110227985777	36.0	36.0	36.0	32.0	36.0
7	33.55407623123582	36.0	36.0	36.0	21.0	36.0
8	33.51968529274532	36.0	36.0	36.0	21.0	36.0
9	33.583008865219696	36.0	36.0	36.0	27.0	36.0
10	33.452395700931575	36.0	36.0	36.0	27.0	36.0
11	33.82335124611845	36.0	36.0	36.0	32.0	36.0
12	33.65576480620405	36.0	36.0	36.0	27.0	36.0
13	33.760397728186895	36.0	36.0	36.0	32.0	36.0
14	33.72438981223754	36.0	36.0	36.0	32.0	36.0
15	33.709800814119994	36.0	36.0	36.0	27.0	36.0
16	33.60604878284234	36.0	36.0	36.0	27.0	36.0
17	33.48769166411919	36.0	36.0	36.0	27.0	36.0
18	33.61330909207923	36.0	36.0	36.0	27.0	36.0
19	33.579778932633985	36.0	36.0	36.0	27.0	36.0
20	33.49629945457178	36.0	36.0	36.0	27.0	36.0
21	33.40905909610156	36.0	36.0	36.0	21.0	36.0
22	33.421600727237625	36.0	36.0	36.0	27.0	36.0
23	33.37999774749409	36.0	36.0	36.0	27.0	36.0
24	33.4195654272521	36.0	36.0	36.0	27.0	36.0
25	32.973874953743184	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	12.0
5	23.0
6	43.0
7	17.0
8	43.0
9	40.0
10	24.0
11	11.0
12	15.0
13	11.0
14	110.0
15	135.0
16	151.0
17	229.0
18	355.0
19	525.0
20	826.0
21	1259.0
22	1724.0
23	2314.0
24	2907.0
25	3382.0
26	4102.0
27	4870.0
28	6110.0
29	7863.0
30	9812.0
31	13248.0
32	18339.0
33	25271.0
34	52272.0
35	92569.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.07341435059476	16.52245272316957	12.086616865589386	21.317516060646287
2	14.250949285622344	22.42687282790578	37.10902304028833	26.21315484618355
3	20.88197198735328	29.082227817958024	26.860604500366048	23.175195694322653
4	11.942069797847733	15.545006537262395	33.59670119682188	38.91622246806799
5	12.626246368530753	39.0122403650381	31.536845832562104	16.824667433869035
6	31.739196922259694	32.79757257720508	18.87651210894428	16.586718391590942
7	26.991333888801677	32.66194116060683	22.060546049533286	18.286178901058207
8	31.052904919299966	27.64816477380238	21.887442680634006	19.411487626263643
9	28.464332015428344	14.127082544871847	20.32620161530595	37.08238382439386
10	17.228034568020554	26.429394093153245	30.20473417578789	26.137837163038313
11	34.447084407216494	23.091978092783506	20.607683634020617	21.85325386597938
12	24.351167174133682	25.341533355887425	29.166465290867205	21.140834179111692
13	31.09105626230776	19.92477418159706	27.08854336559023	21.895626190504956
14	25.780664803434362	18.475398084683103	27.63919875641325	28.104738355469284
15	26.027292457730518	23.152282894498423	30.011636943470226	20.808787704300837
16	24.03601456044841	25.527896788325872	27.846454917372675	22.58963373385304
17	21.664243688511057	24.295557694398692	29.849345795897985	24.190852821192266
18	23.952170568548404	21.09029839024724	33.62357480295933	21.33395623824502
19	22.292880760695773	26.828944135900667	31.082283859379217	19.79589124402434
20	23.538484461644405	19.786888744810145	34.674473765811186	22.000153027734264
21	24.980266125394675	23.155486822604548	28.8179006379277	23.04634641407307
22	22.980963659359873	23.67780812206459	30.033593542306114	23.307634676269426
23	20.729138558074535	23.50650239837611	30.56147374271526	25.202885300834094
24	20.72744407839094	26.802903010141204	30.246401443450104	22.223251468017754
25	23.80973476275241	23.137580243731527	29.003326540639375	24.049358452876692
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	160.0
1	160.0
2	475.0
3	790.0
4	790.0
5	790.0
6	2807.5
7	4825.0
8	4825.0
9	4825.0
10	4427.0
11	4029.0
12	4029.0
13	4029.0
14	2394.5
15	760.0
16	760.0
17	760.0
18	1441.5
19	2123.0
20	2123.0
21	2123.0
22	3502.0
23	4881.0
24	4881.0
25	4881.0
26	6554.0
27	8227.0
28	8227.0
29	8227.0
30	9556.0
31	10885.0
32	10885.0
33	10885.0
34	11110.0
35	11335.0
36	11335.0
37	11335.0
38	12496.0
39	13657.0
40	13657.0
41	13657.0
42	18860.0
43	24063.0
44	24063.0
45	24063.0
46	38158.0
47	52253.0
48	52253.0
49	52253.0
50	44689.5
51	37126.0
52	37126.0
53	37126.0
54	29337.5
55	21549.0
56	21549.0
57	21549.0
58	19405.5
59	17262.0
60	17262.0
61	17262.0
62	15440.5
63	13619.0
64	13619.0
65	13619.0
66	11577.5
67	9536.0
68	9536.0
69	9536.0
70	7302.0
71	5068.0
72	5068.0
73	5068.0
74	4122.0
75	3176.0
76	3176.0
77	3176.0
78	2593.0
79	2010.0
80	2010.0
81	2010.0
82	1349.0
83	688.0
84	688.0
85	688.0
86	511.0
87	334.0
88	334.0
89	334.0
90	218.0
91	102.0
92	102.0
93	102.0
94	62.0
95	22.0
96	22.0
97	22.0
98	77.0
99	132.0
100	132.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00925136357054366
2	0.0016089327948771582
3	0.004022331987192895
4	0.014882628352613713
5	0.03620098788473605
6	0.04746351744887616
7	0.06958634337843708
8	0.08969800331440156
9	0.0957315012951909
10	0.11664762762859394
11	0.11745209402603254
12	0.12871462359017263
13	0.11785432722475181
14	0.11986549321834827
15	0.10659179766061172
16	0.10779849725676958
17	0.11865879362219041
18	0.1258989911991376
19	0.1258989911991376
20	0.11704986082731325
21	0.12388782520554116
22	0.14078161955175134
23	0.12831239039145334
24	0.12791015719273405
25	0.12308335880810259
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	248612.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.57641626309269
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.27403414195868	23.931266391002847
2	14.69781371668164	9.869998230173925
3	5.298592392932015	5.337232313806252
4	2.4917640011979634	3.346580213344488
5	1.4818808026355197	2.4878123340788054
6	0.8948787061994609	1.8028091966598554
7	0.6421084156933213	1.5091789615947742
8	0.4132973944294699	1.110163628465239
9	0.32225217130877504	0.9738065740994
>10	2.0401317759808326	13.51262207777581
>50	0.26235399820305483	6.2161118530079
>100	0.14495357891584307	9.28474892603736
>500	0.016771488469601678	3.99095779769279
>1k	0.017969451931716084	14.541132366901035
>5k	0.0011979634621144056	2.0855791353595157
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5185	2.0855791353595157	No Hit
GTACATGGAAGCAGTGGTATCAACG	4631	1.8627419432690295	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4404	1.7714350071597509	No Hit
TCCATGTACTCTGCGTTGATACCAC	3967	1.5956590993194215	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3407	1.3704085080366193	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3335	1.3414477177288304	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2395	0.9633485109326984	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2214	0.890544301964507	No Hit
CATGTACTCTGCGTTGATACCACTG	2194	0.8824996379901211	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1901	0.7646453107653693	No Hit
CCCATGTACTCTGCGTTGATACCAC	1538	0.6186346596302673	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1533	0.6166234936366708	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1229	0.49434460122600676	No Hit
GCGTTGATACCACTGCTTCCATGTA	1173	0.4718195420977266	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1144	0.46015477933486715	No Hit
GTACATGGGGTGGTATCAACGCAAA	1086	0.4368252538091484	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	995	0.40022203272569307	No Hit
GTATCAACGCAGAGTACATGGGAAG	931	0.3744791080076585	No Hit
GTATCAACGCAGAGTACATGGAAGC	914	0.36764114362943057	No Hit
ACTCTGCGTTGATACCACTGCTTCC	877	0.3527585152768169	No Hit
CAGTGGTATCAACGCAGAGTACATG	788	0.31695976059080017	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	748	0.30087043264202856	No Hit
GTACATGGGTGGTATCAACGCAAAA	661	0.26587614435345036	No Hit
TATCAACGCAGAGTACATGGAAGCA	614	0.24697118401364376	No Hit
GCGTTGATACCACTGCTTCCCATGT	606	0.24375331842388945	No Hit
GTATCAACGCAGAGTACATGGGGTG	573	0.2304796228661529	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	569	0.22887069007127572	No Hit
GAGTACATGGGAAGCAGTGGTATCA	555	0.22323942528920565	No Hit
TATCAACGCAGAGTACATGGGAAGC	549	0.22082602609688995	No Hit
GGTATCAACGCAGAGTACATGGGAA	542	0.2180103937058549	No Hit
GTACATGGGAGTGGTATCAACGCAA	479	0.19266970218653967	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	471	0.18945183659678536	No Hit
ATACCACTGCTTCCATGTACTCTGC	454	0.18261387221855743	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	404	0.16250221228259296	No Hit
TATCAACGCAGAGTACATGGGGTGG	396	0.15928434669283864	No Hit
GGTATCAACGCAGAGTACATGGAAG	393	0.15807764709668076	No Hit
AAAAAGTACTCTGCGTTGATACCAC	374	0.15043521632101428	No Hit
GCTTCCCATGTACTCTGCGTTGATA	372	0.1496307499235757	No Hit
GTATCAACGCAGAGTACATGGGCAG	370	0.1488262835261371	No Hit
GTACTCTGCGTTGATACCACTGCTT	356	0.14319501874406707	No Hit
GTGGTATCAACGCAGAGTACATGGA	352	0.1415860859491899	No Hit
GGTATCAACGCAGAGTACATGGGGT	338	0.13595482116711985	No Hit
CCACTGCTTCCATGTACTCTGCGTT	327	0.13153025598120766	No Hit
GTATCAACGCAGAGTACATGGGAGT	317	0.12750792399401478	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	308	0.12388782520554116	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	290	0.11664762762859394	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT	286	0.11503869483371679	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	284	0.11423422843627822	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	273	0.10980966325036604	No Hit
ACGCAGAGTACATGGGGTGGTATCA	271	0.10900519685292745	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	268	0.10779849725676958	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	264	0.10618956446189241	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	261	0.10498286486573455	No Hit
GTTGATACCACTGCTTCCATGTACT	258	0.10377616526957668	No Hit
ACATGGAAGCAGTGGTATCAACGCA	255	0.10256946567341882	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.022331987192895E-4	0.0
5	0.0	0.0	0.0	4.022331987192895E-4	0.0
6	0.0	0.0	0.0	4.022331987192895E-4	0.0
7	0.0	0.0	0.0	4.022331987192895E-4	0.0
8	0.0	0.0	0.0	4.022331987192895E-4	0.0
9	0.0	0.0	0.0	4.022331987192895E-4	0.0
10	4.022331987192895E-4	0.0	0.0	4.022331987192895E-4	0.0
11	4.022331987192895E-4	0.0	0.0	8.04466397438579E-4	0.0
12	4.022331987192895E-4	0.0	0.0	8.04466397438579E-4	0.0
13	4.022331987192895E-4	0.0	0.0	8.04466397438579E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGGGTA	25	0.006012241	19.003222	9
ATTACCG	25	0.006019239	18.999397	15
CCATAAA	45	3.5205714E-5	16.888351	13
CATAAAC	40	2.7500844E-4	16.624472	14
GCTATTG	35	0.002160855	16.288475	12
CGCTATT	35	0.002160855	16.288475	11
GTAACTA	35	0.0021637643	16.285196	13
TAAAGTG	35	0.002166677	16.28192	5
AATATAC	35	0.002166677	16.28192	5
AAGACGG	50	8.684324E-5	15.196458	5
CAAGACG	50	8.684324E-5	15.196458	4
CAGCCCC	45	6.735701E-4	14.7773075	15
GATTTAA	45	6.735701E-4	14.7773075	1
CCGAGCC	45	6.735701E-4	14.7773075	14
AGGCCCG	40	0.005256679	14.252416	10
ACGCTAT	40	0.005256679	14.252416	10
ATACGCT	40	0.005256679	14.252416	8
TCGCGTA	40	0.005256679	14.252416	9
CCGACCA	60	2.5470901E-5	14.252415	9
TAGTTAG	40	0.005263708	14.249547	18
>>END_MODULE